| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 2,043,578 | 0 | A | C | 55.0% | 4.6 / 15.8 | 23 | intergenic (‑14/+161) | smrA/CJ019_01939 | putative DNA endonuclease SmrA/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (4/5); new base C (0/11); total (4/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.60e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCAGGCCGGCCAGCTTCTGGCGGTCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTAC‑GCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGGATGTCACAGAGCCCGAAGACGGCGG > ACB143/2043446‑2043681 | cgcAGGCCGGCCAGCTTCTGGCGGTCGGCCTTGGGCTTGCCGACTTCGGCACGGGCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAggcg < 3:143689/137‑1 (MQ=255) ggccAGCTTCTGGCGGTCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAggcgg > 7:253589/1‑130 (MQ=255) ggccAGCTTCTGGCGGTCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAggcgg < 8:253589/130‑1 (MQ=255) tgCTTCGGGGGGGCGGCGTTGGGCTGGCCGACTTCGGCAGGGGGGTGGGGGATCGGTTTTACGCCGCGAACTTCGGCCTTCAACAGGTAAAAATCGTCGTCTTGCATGATGCCTCCACGTCGGCGGCGTAGTTAAc‑g < 4:315822/136‑1 (MQ=255) aggttCTGGCGGTCGGCCTTGGGCTTGCCGACTTCGCCACGGTGGGGGCGTTTCGGATTAACCCCGCGCACTTCGGCCTTGACCTGGTAAACAGCGCGTTCTTGCATGATGCCTCCACTTCGGCGGCGTAGTTAAc‑g < 6:160076/134‑1 (MQ=255) ggCGGTCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTTACCAGGTAAAAATCGTCGTCTTCCATGATGCCTCCACGTAGGCGGCGTAGTTAAC‑GCGACtgt < 8:180811/137‑1 (MQ=255) ggggcGGCCTTGGGCTTGCCGACTTCGGCACGGGCGGGGCGGATCGGCTTTACGCCGCGCACTGCGGCCTTGAACCGGGAAAAATCGTCGTCTTGCATGAGGCCCCCACGTCGGCGGCGTAGTTTAC‑GCGACTGTgg < 8:125557/133‑1 (MQ=255) gCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTAC‑GCGACTGTGGGGCGgt < 7:308261/137‑1 (MQ=255) gCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTAC‑GCGACTGTGGGGCGgt > 2:62425/1‑137 (MQ=255) ggggCTTGCGGACTTCGGCTGGGTCGTGGGGTTTCTGTTTTACGCCGCGATCTTGGGCTTTTAACTGGAAAATTTGGTGGTCTTGATTGAGGCCTCCAGGTTGGGGGGTTAGTAACC‑GCGACTGTGGGGCGGTGTGc < 4:4798/136‑1 (MQ=255) tGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTCAACCGGCAAAAAGCGTCGTCTTGCATGATGCCGCCACGTCGGCGGCGTAGTTTAC‑GCGACTGTGGGGCGGTGTGCAGCCtg < 6:80468/137‑1 (MQ=255) gACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTAC‑GCGACTGTGGGGCGGTGTGCAGCCTGTGCg > 3:145244/1‑137 (MQ=255) gACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTAC‑GCGACTGTGGGGCGGTGTGCAGCCTGTGCg > 1:274351/1‑137 (MQ=255) ggCACGGGCGTGGCGGAGCGGCTTAACGCCGCGCACTTCGGCCTTCAACAGGGAAAAATCGTCGTCTTCCATGAGGCCTCCACGTCGGCGGCGTAGTTTCC‑GCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCctc < 7:1052/137‑1 (MQ=255) gCAGGGTGGGGGCGGATCGGCTTAACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTCCATGATGCCTCCAGGTCGGCGGCGTAGTTTCC‑GCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCctct < 2:72648/137‑1 (MQ=255) cGGATCGGCTTAACGCCGCGCAGTTCGGCCTTGAACAGGCAAAAATCTTGGTCTTGCCTGAGGCCTCCACGTAGGGGGCGTATTTACC‑GCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAAc < 7:65528/137‑1 (MQ=255) gtatgggCTTTACGCCGCGCACTGCGGCCTTTAACAGGCAAAAATCGGCGTCTTGCATGATCCCTCCCCGTCGGCGGCGTAGTTAAC‑GCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAAcc < 4:255093/132‑1 (MQ=255) ggggCTATACGCGGCGAAGTTGGTCGTTGAACTGGTAAAAATCGTCGTATTTCATGATGCCACAACGTCGGCGGGTTATTTTAC‑GCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGc < 4:190020/135‑1 (MQ=255) gCTTTCCGCCGCGCACTTGGGCGTTGAACAGGTAAATCTCGTCGTCTTGCATGAGGCCTCCCCGTCGGGGGCGTTGTTTAC‑GCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTcc < 1:289924/137‑1 (MQ=37) ttcaCGCCGCGCACTTCGGCCTGCAACCGGGAAAACTCGTCGTCTTCAATGATGCCTCCACGGCGGGGGCGTAGTTTAC‑GCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCCTa < 6:72840/134‑1 (MQ=37) ccgccgcGCACTTCGGCCTTGAACCGGGAAAAAGCGTCGGCTTGCAGGAGGCCTCCACGTCGGCGGCGTAGTTTAC‑GCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCCTACgg < 6:332458/136‑1 (MQ=38) cgcACTGCGGCATTAAACCTTCAAAAATCGGCGTCTTCCTTGAGGCCGCCCCTTGGGCGGCTTTTTTCAC‑GCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGGATGt < 5:243655/137‑1 (MQ=16) aaaaaaGCGGGGTCTTCGATGATGCCCCCTCTTTGG‑GGGGTTTTTTACGGCTACTGTGGGGCGGTGTGCAGCCGGTGCGGGCCTCTTCGCGGTTGAACCGGCTCCTACGGGGATGTCACAGACCCCGATGAcggcgg < 4:124901/136‑1 (MQ=255) | CGCAGGCCGGCCAGCTTCTGGCGGTCGGCCTTGGGCTTGCCGACTTCGGCACGGTCGTGGCGGATCGGCTTTACGCCGCGCACTTCGGCCTTGAACAGGGAAAAATCGTCGTCTTGCATGATGCCTCCACGTAGGCGGCGTAGTTTAC‑GCGACTGTGGGGCGGTGTGCAGCCTGTGCGGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGGATGTCACAGAGCCCGAAGACGGCGG > ACB143/2043446‑2043681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |