Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,440,3310TG52.0% 3.6 / 17.9 28A19A (GCT→GCG) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (11/1);  new base G (9/4);  total (23/5)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.50e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGG  >  ACB143/3440200‑3440451
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cAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCgcgggcgg                                                                                                                     >  7:218037/1‑137 (MQ=255)
     gTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGccc                                                                                                                >  7:69666/1‑137 (MQ=255)
                    gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGgtgt                                                                                                 >  4:251459/1‑137 (MQ=255)
                         ggTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCCCGCCGCCGGCGCCCCGGCCCCCGGGGGGTgggcg                                                                                            >  3:43110/1‑134 (MQ=255)
                              ggaggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCaa                                                                                       >  5:85540/1‑137 (MQ=255)
                              ggaggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCCCCTGGGGGTGGCCGGCCac                                                                                       >  1:150292/1‑136 (MQ=255)
                                       cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGGGGGGGGGGCCCACGCCTATaa                                                                              >  5:247707/1‑137 (MQ=255)
                                        aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCTCGCGGCGGCCGGGCCGCCGGGGTGTGGCCGGCCACCGCCtagact                                                                             >  6:238544/1‑133 (MQ=255)
                                        aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCCCCGCCGATaac                                                                             >  3:278882/1‑136 (MQ=255)
                                        aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGCGCGGCGCCCCGGCCCCCTGGGGGGGGCAGGCCACCGccgaaaaa                                                                             >  3:61611/1‑131 (MQ=255)
                                          ggCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCACGCGGGCGGCCCCCGGCGCGCCCGGGGGTGGCTCGCCAAAGCCGATAAggg                                                                           >  6:82402/1‑136 (MQ=255)
                                                     cgcgagcgCTCGAGGTGCCGCAGTGAGCGCTGGGGCCACCCGCCTGGGCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTg                                                                <  8:246052/136‑1 (MQ=255)
                                                         agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCAGGCGGCCGCCGGGCGGCCTGGGGGTGGCCGGCCCCCCCCTTGAAGGCGCAGGGCGCGGATCa                                                            >  6:83222/1‑137 (MQ=255)
                                                         agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCTCGGTGTGGCCGGCCCACGCCGAAAAGGAGGCGCTCGCTGCTCa                                                            >  3:19266/1‑137 (MQ=255)
                                                         agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCGGGGGGTGGCGGGCCAACGCCGATAAGGCGCAGCTCGCTGATCa                                                            >  5:322261/1‑137 (MQ=255)
                                                         agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGGAGGCCAACGCCGATAAGGCGCAGCTCGCTGCTCa                                                            >  5:373609/1‑137 (MQ=255)
                                                            gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGGGGGAGGCCCACGCCGATAAGGCGCCGCGCGCGGATGAGGc                                                         >  3:214659/1‑137 (MQ=255)
                                                            gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCAGCCTGGGTGTGGCAGGCCAACGCCGATAAGGCGCAGCTAGCTGATCAGGc                                                         >  4:26083/1‑137 (MQ=255)
                                                            gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGGGGGCCCACCCCGCAAAGGCGCCCCTCGCGGAGCGGGc                                                         >  1:359132/1‑137 (MQ=255)
                                                            gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTTGCAGGCCACCCCCGATAAGGCGCCGCTCGCGGATCAGGc                                                         >  7:98574/1‑137 (MQ=255)
                                                            gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCCCGCAGGCGGCGCCCCGGCCGCCGGGGTGTGGCAGCCCCCCGCCTATAAGGCGCCGCGCCCGGAGCAggg                                                         >  2:350772/1‑136 (MQ=255)
                                                              tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCGCCTGGGGGTGGGCGGCCACCGCCGATAAGGCGCAGCTCGCGGATCAGGGCg                                                       >  3:215226/1‑137 (MQ=255)
                                                                   gTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCGGGCGGCGCCCGGGCCGCCCGGGGGGGGCCGGCCCACGCCCATTATGCGCCCGGCGCGGCTCAGGCCGCTggc                                                  >  2:283440/1‑135 (MQ=255)
                                                                                gggtggggCCACCCGCCGGGGCGCCGGGGCGCGGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGt                                     <  7:217955/132‑1 (MQ=255)
                                                                                 gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGGGGCGCCCCGGCCGCCGGGGGGTGGGAGGGCCACGCCTATAAGGCGCCGCTCGCTGAGCAGGCCGATGGCTTCGGGGAGCAGtt                                    >  5:364969/1‑137 (MQ=255)
                                                                                 gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGTGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGtt                                    >  8:108011/1‑137 (MQ=255)
                                                                                                        ggTGGCGGGGGTGCTGGCCGCCCGCGGGGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACg             <  2:38686/136‑1 (MQ=255)
                                                                                                        cGTGGCGCTGCTGCTGGCCGCCAGCGCTGGTGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACg             <  7:108011/137‑1 (MQ=255)
                                                                                                                   ttcgtgccgccGGCGGGGGCGCCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAATCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCgg  <  4:88982/132‑1 (MQ=255)
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CAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGG  >  ACB143/3440200‑3440451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑