Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,440,3460AC56.7% 10.7 / 30.0 31A24A (GCA→GCC) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (4/9);  new base C (17/0);  total (22/9)
Fisher's exact test for biased strand distribution p-value = 5.00e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.02e-04
Rejected as consensus: Frequency below/above cutoff threshold.

GCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCA  >  ACB143/3440220‑3440480
                                                                                                                              |                                                                                                                                      
gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGgtgt                                                                                                                              >  4:251459/1‑137 (MQ=255)
     ggTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCCCGCCGCCGGCGCCCCGGCCCCCGGGGGGTgggcg                                                                                                                         >  3:43110/1‑134 (MQ=255)
          ggaggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCaa                                                                                                                    >  5:85540/1‑137 (MQ=255)
          ggaggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCCCCTGGGGGTGGCCGGCCac                                                                                                                    >  1:150292/1‑136 (MQ=255)
                   cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGGGGGGGGGGCCCACGCCTATaa                                                                                                           >  5:247707/1‑137 (MQ=255)
                    aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCTCGCGGCGGCCGGGCCGCCGGGGTGTGGCCGGCCACCGCCtagact                                                                                                          >  6:238544/1‑133 (MQ=255)
                    aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCCCCGCCGATaac                                                                                                          >  3:278882/1‑136 (MQ=255)
                    aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGCGCGGCGCCCCGGCCCCCTGGGGGGGGCAGGCCACCGccgaaaaa                                                                                                          >  3:61611/1‑131 (MQ=255)
                      ggCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCACGCGGGCGGCCCCCGGCGCGCCCGGGGGTGGCTCGCCAAAGCCGATAAggg                                                                                                        >  6:82402/1‑136 (MQ=255)
                                 cgcgagcgCTCGAGGTGCCGCAGTGAGCGCTGGGGCCACCCGCCTGGGCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTg                                                                                             <  8:246052/136‑1 (MQ=255)
                                     agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCAGGCGGCCGCCGGGCGGCCTGGGGGTGGCCGGCCCCCCCCTTGAAGGCGCAGGGCGCGGATCa                                                                                         >  6:83222/1‑137 (MQ=255)
                                     agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCTCGGTGTGGCCGGCCCACGCCGAAAAGGAGGCGCTCGCTGCTCa                                                                                         >  3:19266/1‑137 (MQ=255)
                                     agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCGGGGGGTGGCGGGCCAACGCCGATAAGGCGCAGCTCGCTGATCa                                                                                         >  5:322261/1‑137 (MQ=255)
                                     agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGGAGGCCAACGCCGATAAGGCGCAGCTCGCTGCTCa                                                                                         >  5:373609/1‑137 (MQ=255)
                                        gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGGGGGAGGCCCACGCCGATAAGGCGCCGCGCGCGGATGAGGc                                                                                      >  3:214659/1‑137 (MQ=255)
                                        gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCAGCCTGGGTGTGGCAGGCCAACGCCGATAAGGCGCAGCTAGCTGATCAGGc                                                                                      >  4:26083/1‑137 (MQ=255)
                                        gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGGGGGCCCACCCCGCAAAGGCGCCCCTCGCGGAGCGGGc                                                                                      >  1:359132/1‑137 (MQ=255)
                                        gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTTGCAGGCCACCCCCGATAAGGCGCCGCTCGCGGATCAGGc                                                                                      >  7:98574/1‑137 (MQ=255)
                                        gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCCCGCAGGCGGCGCCCCGGCCGCCGGGGTGTGGCAGCCCCCCGCCTATAAGGCGCCGCGCCCGGAGCAggg                                                                                      >  2:350772/1‑136 (MQ=255)
                                          tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCGCCTGGGGGTGGGCGGCCACCGCCGATAAGGCGCAGCTCGCGGATCAGGGCg                                                                                    >  3:215226/1‑137 (MQ=255)
                                               gTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCGGGCGGCGCCCGGGCCGCCCGGGGGGGGCCGGCCCACGCCCATTATGCGCCCGGCGCGGCTCAGGCCGCTggc                                                                               >  2:283440/1‑135 (MQ=255)
                                                            gggtggggCCACCCGCCGGGGCGCCGGGGCGCGGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGt                                                                  <  7:217955/132‑1 (MQ=255)
                                                             gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGGGGCGCCCCGGCCGCCGGGGGGTGGGAGGGCCACGCCTATAAGGCGCCGCTCGCTGAGCAGGCCGATGGCTTCGGGGAGCAGtt                                                                 >  5:364969/1‑137 (MQ=255)
                                                             gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGTGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGtt                                                                 >  8:108011/1‑137 (MQ=255)
                                                                                    ggTGGCGGGGGTGCTGGCCGCCCGCGGGGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACg                                          <  2:38686/136‑1 (MQ=255)
                                                                                    cGTGGCGCTGCTGCTGGCCGCCAGCGCTGGTGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACg                                          <  7:108011/137‑1 (MQ=255)
                                                                                               ttcgtgccgccGGCGGGGGCGCCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAATCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCgg                               <  4:88982/132‑1 (MQ=255)
                                                                                                            ggggggcggcGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcag                  <  8:218037/133‑1 (MQ=255)
                                                                                                             gggggcggcGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcagc                 <  4:278882/134‑1 (MQ=255)
                                                                                                                            gCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGTGGGAGAAGGCTGCAGCAGCAGCGCTCGATCa  <  2:89462/137‑1 (MQ=255)
                                                                                                                            gCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGATCGCTGATCAGGACGATGACTTCGGCAAGCAGTTGGCGGAGATGTATCGCGCGCACGGTCTGGTGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCa  <  5:120653/137‑1 (MQ=255)
                                                                                                                              |                                                                                                                                      
GCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCA  >  ACB143/3440220‑3440480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑