Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1436,129,7950TC27.3% 36.8 / 13.6 42T418A (ACC→GCC) copA_4Copper resistance protein A
Reads supporting (aligned to +/- strand):  ref base T (11/13);  new base C (3/6);  total (17/25)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGCCGGCCATGCTGC  >  ACB143/6129663‑6129923
                                                                                                                                    |                                                                                                                                
ggTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATACATGCCGGCCATGCTGCCATGGTCCATACCAGCCATTTTGCTGTGGTCCATGCCGGTCATg                                                                                                                              >  6:141448/1‑137 (MQ=255)
  tCCATCCCAGCCAACTTGCTGTGGTCCATGCCTGACATGCCGCCGTGGTCCATCTCATCCATATTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGtt                                                                                                                            <  1:99636/137‑1 (MQ=17)
  cccATCCCAGCCATCTTTCTGTGGTCCATTCCGGCCATGCCTCCCTGGTCCATACCAGCCATTTTGCTGTGGTCCATGTCGGCCATGCAGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGtt                                                                                                                            <  6:92979/136‑1 (MQ=11)
  accATCACATCCCTCTTGCTGTTGTCCATGCCGGCCATGCCGCCGTGGTGCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGtt                                                                                                                            <  1:94641/136‑1 (MQ=17)
   ccATACCATCCATCTTGCTGTGGTCCATTGCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGTTCCATGGCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTg                                                                                                                           <  2:125696/137‑1 (MQ=17)
       cccATCCATCTTGCTGGGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGt                                                                                                                       <  6:178889/137‑1 (MQ=255)
         cAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTgg                                                                                                                     >  8:53839/1‑137 (MQ=255)
         cAGCCATCTTGCTGTGGTCCATGCCGGCCATGCAGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGACATTTTGCTGTGGTCCATACCGGTCATGTTGCCGTgg                                                                                                                     >  7:248217/1‑137 (MQ=255)
         cAGCCATCTTGCTGTGGTCCATGCCGGCCATGCAGCCGTGGTCCATCACCGCCATTATGCGGTGATCCATCCCGGCCAGTCTGTCATGGTCCATACCTGCAAATTTGCATTGCTCAATGGCGGCAATGTTTCAGTgg                                                                                                                     >  4:86420/1‑137 (MQ=9)
         cAGCCATCTTGCTGTGGTCCATGCCGGCCATGAGGCCGTGGTCCATCACCGCCATTATGCTGTGATCCATGCCGGCCATGCTGCCTTGTTACATCCACTCCAGATTGCTGTGTTACAAGCCGGGAAGATTGCCGTgg                                                                                                                     >  6:88225/1‑137 (MQ=9)
          aGCCATCTTGCTGTGGTCCATGCCGGCCATGCAGCCGTGGTCCATCACAGCCATTTTTCTCTGTTACATGCAGGCCATGATGACTTGGGCCATCCCCGCCGTTTTGCGAGGGTCCATTCAGGACATggtggggtggg                                                                                                                    >  6:36524/1‑136 (MQ=9)
                cTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGCTCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATcc                                                                                                              <  3:305312/137‑1 (MQ=255)
                cTTGCTCTGGTCCATCGCGGCCATGCCGCCGTGGTCCATCTCAGCCATCTTGCTGTGCTCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATATTGCTGTGGTCCATGCCGGGCATGTTGCCGTGGTCCATcc                                                                                                              <  8:352209/137‑1 (MQ=17)
                          gccATGCGGGCCATGCCGCCTTGGTCCATCACAGCCATTTTGCTGTGTTCCATGGCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCAtttt                                                                                                    <  2:323745/136‑1 (MQ=14)
                               gCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCtgt                                                                                               >  7:52721/1‑137 (MQ=255)
                                ccGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCtgtg                                                                                              <  2:200683/137‑1 (MQ=255)
                                 cGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATTCCGGTCATGTTGCAGTGGTCCATCCCTGCCATGTTGGTGTgg                                                                                             >  3:277514/1‑137 (MQ=17)
                                           gccgTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCgg                                                                                   >  8:51546/1‑137 (MQ=255)
                                                  tacATCTCAGCCATCTTGCTGTGTTCCATGCCGGCCATGCTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGtt                                                                            <  5:252497/135‑1 (MQ=11)
                                                  tCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGtt                                                                            <  7:51546/137‑1 (MQ=255)
                                                   ccATCTCACCCATTTTGCTGTGATCCATGACTGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGGCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTg                                                                           <  4:277514/137‑1 (MQ=255)
                                                              aTTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTATGCTGTGGTCCATGCCGGCCATGTTGCAGTGGTCCAt                                                                >  3:299654/1‑137 (MQ=255)
                                                                tttGCTGTGGTCCATGCAGCCCATGATGCCATGCTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGATGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATcc                                                              <  7:360471/137‑1 (MQ=14)
                                                                          tCCTTGCCGGACATGCTGCCTTCGTCCATCGCATCCATTTTGCGGTTGTCCATGGCGGTCATCTTGCCGTGGTCCATCCCTGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAttta                                                    <  5:20413/137‑2 (MQ=14)
                                                                          tCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttt                                                    <  3:355953/137‑1 (MQ=255)
                                                                          tCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGGCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttt                                                    <  7:20440/137‑1 (MQ=255)
                                                                          cccATCCCGGCCATGCTGCCTAGGTCCATACCAGCCATGTTGCTGTGGTCCATGCCGGACATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttt                                                    <  1:327423/136‑1 (MQ=14)
                                                                                 cGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTgg                                             >  7:246261/1‑137 (MQ=255)
                                                                                                ggTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATACCAGCCAGTTTGGTGTGGTCCATACCGGGCATg                              >  4:171559/1‑137 (MQ=255)
                                                                                                   ccAACCGAGCCTTTTTGCTTGGCGCCATGCGCGACATGTTGCCGTGGGCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGATGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTg                           <  2:248387/137‑1 (MQ=14)
                                                                                                       cccAGCCATGTTGCTGTGGTCCATGCCCGCCATGACGCCGTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGt                       <  8:239412/137‑1 (MQ=14)
                                                                                                         ccgCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGACATGTTGCCGTGGTCGATCCCCGCCAGTTTGCTGTGGTCCATACCGGCCATGTTGCCgggg                     >  1:316334/3‑137 (MQ=14)
                                                                                                         cAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCCGCCATTTTGCTGTGGTCCATGCCGGCCAGGTTGCCGTGGTCCAGCCCCGCCATTTTGCTGTCGTCCATACCGGCCATGTTGCCGTgg                     >  4:139314/1‑137 (MQ=14)
                                                                                                         cAGCCATTTTGCTGTGGTCCATGCCGGGCATGTTGCCGTGGTCCATCCCCGCCATTTTGCGGTGGTCCATGCCGGCAATGTTGCCGTGGTGCATCCCCCCCATTTTGCAGTCGTCCATACCGGCCAGGTTGCCGtcg                     >  5:4837/1‑135 (MQ=17)
                                                                                                         cAGCCAGTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATGATGCTGGGGTCCAGGACGCCCCAGGTGCCGTGGACCATGCCTGCCATTTTGATGAGGTCCATACGGGCCATGGGGCCGCgg                     >  6:82889/1‑137 (MQ=9)
                                                                                                               ttttGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATg               >  3:174386/1‑137 (MQ=255)
                                                                                                                tttGCTGTGGTCGATGACGGTCATGTTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGACATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGc              <  4:299654/137‑1 (MQ=255)
                                                                                                                tttGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGGCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGc              <  5:283712/137‑1 (MQ=255)
                                                                                                                 ttGCTGTGGTCCCTGCCGTGCCTGTTGCCGTGGTCCATCGCAGCCATTTTGCGGTGGTCCATGCCGGCCTTGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGcc             <  5:82889/137‑1 (MQ=14)
                                                                                                                           ccATGCCGGCCATGCTGCCCTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGCCGGCCAtgctg   <  5:75771/137‑1 (MQ=255)
                                                                                                                           ccATGCCCGCCATGGTGCCGTGGTCCATCCCAGCCATCTTGCTGTGGTCCATCCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGCCGGCCAtgctg   <  8:195915/137‑1 (MQ=14)
                                                                                                                            cATGCCGGCCATGCTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGGCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGCCGGCCAtgctgc  <  6:138364/137‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                
GGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGCCGGCCATGCTGC  >  ACB143/6129663‑6129923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑