Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,386,517	G→C	intergenic (‑526/+1125)	CJ019_05070 ← / ← nptII	hypothetical protein/neomycin phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,386,517	0	G	C	100.0%	72.4 / NA	21	intergenic (‑526/+1125)	CJ019_05070/nptII	hypothetical protein/neomycin phosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (5/16);  total (5/16)

TATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑C‑A‑GGATC‑‑ATCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTCC  >  ACB143/5386417‑5386609                                                                                                            |                                                                                             tATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGaa                                                              <  5:248219/137‑1 (MQ=255)      aTAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCa                                                         >  6:53047/1‑137 (MQ=255)        aaCAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCAcc                                                       >  3:272699/1‑137 (MQ=255)              aaTTGATCTAATTCTTTCATTAAAGACCCAAATATGACATCTCGGCCGGCTTGGCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                 <  5:253052/137‑1 (MQ=255)                 tGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                              <  1:118112/137‑1 (MQ=255)                     cGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttt                                          >  5:99973/1‑137 (MQ=255)                      taaTTCTTTCATTTATGACCCTAATATGCCATCTCGGCCGGCTGGTCTACGACGGCGGTCTCAGT‑‑GGG‑CTACGGTACAAACCCCGACGCTAGCAACGCATGAGAAACCCCCCGGAAGATCACCTTCCTGGGGCtttt                                         <  4:247285/136‑1 (MQ=255)                       aaTTCTTTCATTTACCACCATACAATTACACCTCGGCCGGCTTGGCGACGACGGGGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttttt                                        <  4:215125/137‑1 (MQ=255)                             ttCATTTACGACCCAAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGGGT‑A‑C‑GGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgc                                  <  8:250365/137‑1 (MQ=255)                              tCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                 <  6:99973/137‑1 (MQ=255)                               aaTTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCATT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                                <  1:231046/136‑1 (MQ=255)                               aaTTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                                <  6:112873/136‑1 (MQ=255)                                            aaTATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg                   <  4:141415/137‑1 (MQ=255)                                             atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCtt                                                     >  4:319199/1‑102 (MQ=255)                                             atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCtt                                                     <  3:319199/102‑1 (MQ=255)                                              tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc                 <  6:157243/137‑1 (MQ=255)                                               atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCg                >  4:48497/1‑137 (MQ=255)                                                 gACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTg              <  4:184590/137‑1 (MQ=255)                                                  aCATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGttc             <  5:53047/137‑3 (MQ=255)                                                           ccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGACt    <  5:189182/137‑1 (MQ=255)                                                             ggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTGT‑ACGGTACAAACCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTcc  <  4:272699/137‑1 (MQ=255)                                                                                                            |                                                                                             TATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑C‑A‑GGATC‑‑ATCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTCC  >  ACB143/5386417‑5386609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑