Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,396,806 T→A 9.1% E643D (GAA→GAT)  mcpU_1 ← Methyl‑accepting chemotaxis protein McpU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,396,8060TA 9.1% 133.6 / 5.2 44E643D (GAA→GAT) mcpU_1Methyl‑accepting chemotaxis protein McpU
Reads supporting (aligned to +/- strand):  ref base T (17/23);  new base A (2/2);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

CGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCGCCAATACGCTGCAGTGCCGGTAGTGCCTGCTCCATGCGTGCGGCGCTGTCCTGG  >  ACB143/1396673‑1396938
                                                                                                                                     |                                                                                                                                    
cgcgAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTc                                                                                                                                    <  2:186567/136‑1 (MQ=255)
cgcgAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTc                                                                                                                                    <  5:94806/136‑1 (MQ=255)
cgcgAAACTGCTCCAGCAGCGCCGGCTGCTGGATGGCCAGCCGGTTCAGGGCATGGCGCATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATGCTGGCACGTCTCGACACCCGGCCACGTTACGGTTCACTTc                                                                                                                                    <  3:231421/136‑1 (MQ=255)
  cgAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACATCCt                                                                                                                                  >  3:157719/1‑136 (MQ=255)
  cgAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACATCCt                                                                                                                                  >  3:153817/1‑136 (MQ=255)
     aaCTGCTCCATCAGCGCCGGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCgg                                                                                                                               <  5:66723/136‑1 (MQ=255)
       cTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGcc                                                                                                                             >  4:155206/1‑136 (MQ=255)
          cTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCAcc                                                                                                                          <  7:242454/136‑1 (MQ=255)
          cTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACATCCTCGGCCAcc                                                                                                                          <  4:157719/136‑1 (MQ=255)
          cTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACATCCTCGGCCAcc                                                                                                                          <  4:153817/136‑1 (MQ=255)
              aTCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCAc                                                                                                                      >  3:101185/1‑136 (MQ=255)
                  gcgcCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTg                                                                                                                  >  3:93539/1‑136 (MQ=255)
                       tgctgctgGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCtt                                                                                                             >  1:118340/1‑136 (MQ=255)
                        gctgctgGTTGGCCAGCCGGTTCAGGGCTTGGCTGAAGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTc                                                                                                            >  3:29554/1‑136 (MQ=255)
                            ctgGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTc                                                                                                                                    >  6:120052/1‑108 (MQ=255)
                            ctgGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTc                                                                                                                                    <  5:120052/108‑1 (MQ=255)
                             tgGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTccgccg                                                                                                       <  7:219178/136‑1 (MQ=255)
                                       gCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTag                                                                                             >  4:13556/1‑135 (MQ=255)
                                                      ggCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATg                                                                                <  2:248414/134‑1 (MQ=255)
                                                         tGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCt                                                                           >  4:130168/1‑136 (MQ=255)
                                                         tGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTAACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCt                                                                           >  2:231658/1‑136 (MQ=255)
                                                           aTGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCt                                                                           <  8:217809/134‑1 (MQ=255)
                                                              cGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCa                                                                      >  3:192512/1‑136 (MQ=255)
                                                              cGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCa                                                                      >  1:3599/1‑136 (MQ=255)
                                                                tGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCAcc                                                                    <  2:291488/136‑1 (MQ=255)
                                                                tGCCGATTCATCGGCCGGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCAcc                                                                    <  2:266679/136‑1 (MQ=255)
                                                                   cGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGcc                                                                 >  8:221475/1‑136 (MQ=255)
                                                                     aTTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCAc                                                               <  3:240650/136‑1 (MQ=255)
                                                                     aTTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCAc                                                               <  7:187064/136‑1 (MQ=255)
                                                                     aTTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCAGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCAc                                                               >  4:297129/1‑136 (MQ=255)
                                                                            ggCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCg                                                        <  3:111712/136‑1 (MQ=255)
                                                                            ggCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCg                                                        <  6:256262/136‑1 (MQ=255)
                                                                                     agccagTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCGCCAATACGc                                               <  2:73887/136‑1 (MQ=255)
                                                                                         agTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCGCCAATACGCTGCa                                           <  4:93539/136‑1 (MQ=255)
                                                                                               tCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCGCCAATACGCTGCAGTGCCg                                     >  3:219802/1‑136 (MQ=255)
                                                                                                  gTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCGCCAATACGCTGCAGTGCCgg                                    >  1:226044/1‑134 (MQ=255)
                                                                                                     aCGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCGCCAATACGCTGCAGTGCCGGTAGt                                <  3:28313/135‑1 (MQ=255)
                                                                                                                gCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCGCCAATACGCTGCAGTGCCGGTAGTGCCTGCTCCAtg                    >  3:16793/1‑136 (MQ=255)
                                                                                                                    gCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCGCCAATACGCTGCAGTGcc                                      <  6:156550/114‑1 (MQ=255)
                                                                                                                    gCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCGCCAATACGCTGCAGTGcc                                      >  5:156550/1‑114 (MQ=255)
                                                                                                                      cACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCGCCAATACGCTGCAGTGCCGGTAGTGCCTGCTCCAtgcgtgcg              <  6:5273/136‑1 (MQ=255)
                                                                                                                      cACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCGCCAATACGCTGCAGTGCCGGTAGTGCCTGCTCCAtgcgtgcg              <  8:114723/136‑1 (MQ=255)
                                                                                                                                   aCTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCGCCAATACGCTGCAGTGCCGGTAGTGCCTGCTCCATGCGTGCGGCGCTGTCCTgg  <  2:3599/135‑1 (MQ=255)
                                                                                                                                   aCTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCGCCAATACGCTGCAGTGCCGGTAGTGCCTGCTCCATGCGTGCGGCGCTGTCCTgg  <  4:29554/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
CGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCCACGTTACGGTTCACTTCCTCGGCCACCGCACTCTGCTCTTCCGCCGCCGAGGCTATCTGCAAGTTCATGTCGCTGATCACCGCCACGGCCTCGCCAATACGCTGCAGTGCCGGTAGTGCCTGCTCCATGCGTGCGGCGCTGTCCTGG  >  ACB143/1396673‑1396938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑