Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,800,374 C→T 8.9% I247I (ATC→ATT)  rihA → Pyrimidine‑specific ribonucleoside hydrolaseRihA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,800,3740CT 8.9% 139.8 / 3.7 45I247I (ATC→ATT) rihAPyrimidine‑specific ribonucleoside hydrolaseRihA
Reads supporting (aligned to +/- strand):  ref base C (15/26);  new base T (2/2);  total (17/28)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCAGCGGCCGGCGTATCCACATGAGCATCGACAGCCGCGAAGGACCCACT  >  ACB143/2800239‑2800509
                                                                                                                                       |                                                                                                                                       
cAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATc                                                                                                                                         <  1:26376/136‑1 (MQ=255)
cAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATc                                                                                                                                         <  2:254925/136‑1 (MQ=255)
 aGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCCAGCGCGTGGTGGATATCCTCAATGCCGACATCa                                                                                                                                        >  2:158909/1‑136 (MQ=255)
  gcggcgTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCAc                                                                                                                                       <  1:988/136‑1 (MQ=255)
       gTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACg                                                                                                                                  <  7:20331/136‑1 (MQ=255)
       gTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACg                                                                                                                                  <  3:50594/136‑1 (MQ=255)
       gTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACg                                                                                                                                  <  5:33549/136‑1 (MQ=255)
       gTGAAGCTGACCTACATGCAGGTGGATGTCACGCTCAAGCTTCTGACCAGTAACTCGCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGTATATCCTCAATTCCTACATCACCCACg                                                                                                                                  <  5:97774/136‑1 (MQ=255)
          cAGCTGACCTACCTGCCGCTGGATGTCACGCACATGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCATCCTTGAACAACCAGGCGAGCAAGCGCGTGGTGTATATCCTCAATGCCTACATTACCCACGACa                                                                                                                               <  3:59187/136‑1 (MQ=255)
          cAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACa                                                                                                                               <  2:268535/136‑1 (MQ=255)
           aGCTGACCTACCTGCCGCTGGATGTCACGCACCAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACAt                                                                                                                              >  3:135499/1‑136 (MQ=255)
           aGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATTACCCACGACa                                                                                                                               >  4:63299/1‑135 (MQ=255)
           aGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATTACCCACGACa                                                                                                                               <  3:63299/135‑1 (MQ=255)
           aGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACAt                                                                                                                              >  7:266024/1‑136 (MQ=255)
           aGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCAGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGAAATCCTCAATGCCTACATCACCCACGACAt                                                                                                                              >  8:139293/1‑136 (MQ=255)
           aGCTGACCTACCTGCCGCGGGATGTCACGCACAAGCGGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGCACAACCAGGCGAGCAAGCGCGGGGGGGATATCCTCACTGCCTACATTACCCACGACAt                                                                                                                              >  4:59187/1‑136 (MQ=255)
            gCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATg                                                                                                                             <  8:51803/136‑1 (MQ=255)
                acctacctGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGAc                                                                                                                          <  3:125255/135‑1 (MQ=255)
                acctacctGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACg                                                                                                                         <  2:118216/136‑1 (MQ=255)
                               gATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGTCTTCATCACACACGACATGGACGTGTATGGCATACCTg                                                                                                          <  4:260275/136‑1 (MQ=255)
                               gATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTg                                                                                                          <  7:123547/136‑1 (MQ=255)
                                  gTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGTCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTggcg                                                                                                       <  2:120589/136‑1 (MQ=255)
                                       gCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCt                                                                                                  >  7:254514/1‑136 (MQ=255)
                                                    aCCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGcc                                                                                      <  1:48318/135‑1 (MQ=255)
                                                       aGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCg                                                                                  <  8:221401/136‑1 (MQ=255)
                                                            cgcccgccTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATc                                                                             >  6:187668/1‑136 (MQ=255)
                                                               ccgccTGAAGCAGTTGGCAGCCGTGAACAACCAGGAGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGcc                                                                          <  7:70569/136‑1 (MQ=255)
                                                                 gccTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGCCGCCAGCGTCATCGCCTa                                                                        >  4:170544/1‑136 (MQ=255)
                                                                 gccTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGAGATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTa                                                                        >  7:107509/1‑136 (MQ=255)
                                                                          aGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTaac                                                               >  2:69187/1‑135 (MQ=255)
                                                                            ttGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAg                                                              <  7:153847/135‑1 (MQ=255)
                                                                               gCAGCCGTGAACAACCAGGCGATCAAGCGCGTGGTGGATATCCTCAATGCCTTCATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGccc                                                           <  7:139293/135‑1 (MQ=255)
                                                                                        aacaacCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCa                                                 <  8:266024/136‑1 (MQ=255)
                                                                                         acaacCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCAg                                                <  2:262912/136‑1 (MQ=255)
                                                                                         acaacCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCAg                                                <  3:177130/136‑1 (MQ=255)
                                                                                         acaacCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTGCAg                                                >  2:28658/1‑136 (MQ=255)
                                                                                                ggCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCAGcggccgg                                         >  5:51378/1‑136 (MQ=255)
                                                                                                 gggAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACGTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCAGcggccggc                                        <  6:27016/134‑1 (MQ=255)
                                                                                                       aaGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCAGCGGCCGGCGTATcc                                  <  3:147865/136‑1 (MQ=255)
                                                                                                         gcgcGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTg                                                                                                          <  1:217158/62‑1 (MQ=255)
                                                                                                         gcgcGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTg                                                                                                          >  2:217158/1‑62 (MQ=255)
                                                                                                                        ccTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCAGcggccggc                                        <  2:167289/113‑1 (MQ=255)
                                                                                                                        ccTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCAGcggccggc                                        >  1:167289/1‑113 (MQ=255)
                                                                                                                            aaTGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCAGCGGCCGGCGTATCCACATGAGCATCGACAGCcgcg             <  5:168145/136‑1 (MQ=255)
                                                                                                                              tGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCAGCGGCCGGCGTATCCACATGAGCATCGACAGCcgcg             >  4:177511/1‑134 (MQ=255)
                                                                                                                                ccTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCAGcg                                              >  7:242609/1‑99 (MQ=255)
                                                                                                                                ccTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCAGcg                                              <  8:242609/99‑1 (MQ=255)
                                                                                                                                       cACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCAGCGGCCGGCGTATCCACATGAGCATCGACAGCCGCGAAGGACCCACt  >  1:59477/1‑136 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CAGCGGCGTGCAGCTGACCTACCTGCCGCTGGATGTCACGCACAAGCTGCTGACCAGTAACGCCCGCCTGAAGCAGTTGGCAGCCGTGAACAACCAGGCGAGCAAGCGCGTGGTGGATATCCTCAATGCCTACATCACCCACGACATGGACGTGTATGGCATACCTGGCGGCCCTGTGCACGACGCCAGCGTCATCGCCTACCTGTTAAAGCCCGAGCTGTTCAGCGGCCGGCGTATCCACATGAGCATCGACAGCCGCGAAGGACCCACT  >  ACB143/2800239‑2800509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑