Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,245,128 A→C 22.8% L178R (CTG→CGG)  CJ019_03982 ← Crotonyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,245,1280AC22.8% 40.5 / 2.5 52L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (9/23);  new base C (9/3);  total (18/34)
Fisher's exact test for biased strand distribution p-value = 7.17e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

CGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGAA  >  ACB143/4244993‑4245261
                                                                                                                                       |                                                                                                                                     
cGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCa                                                                                                                                       >  5:45895/1‑136 (MQ=255)
  ggCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCGTCGCTGCCCAACCCGGCGGCCCgg                                                                                                                                     >  2:172176/1‑136 (MQ=255)
  ggCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAgg                                                                                                                                     <  3:216088/136‑1 (MQ=255)
      ccGTCGCGTATAAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTACGCTGACCAACCCGGCGGCCAcgccgc                                                                                                                                 >  2:49513/1‑136 (MQ=255)
                     cGCTTGGTGTGCGCTTCGCCGAAGGTCGGGCCTTTGCCCAGGCGCCGGGCCTGTGGGGCGGCAACGGCTGGCAAGTGCGCGGCGGGGCAGACTTCGCTGACCAACCCGGCGGCCTGGGCGCGAGGGGCGCCCcaca                                                                                                                  <  7:51907/136‑1 (MQ=255)
                                ctaaTAGGCGAAGGTCGGCCCTTTGGCCTGGCGCCCGGCCTGTTCGGCGGCAAGGGCAGGAAACTGCTCGTCGGCGCAGAGTTCGCTGACCAACCGGGCGGCCTGGGCGCTATCGGCGCCCCACAACtcgtcg                                                                                                          <  5:98436/130‑1 (MQ=255)
                                cgcATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACtcgtcg                                                                                                          <  7:41505/133‑1 (MQ=255)
                                   aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTACAGGAAc                                                                                                    >  2:97496/1‑136 (MQ=255)
                                   aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCACGGCGCGAAAGGCGCCCCACCACACGGCGAGGAAc                                                                                                    >  3:247545/1‑136 (MQ=255)
                                    tAGGCGATGGTCGGGCCATTGGCCTGGCGCCCGGCCAGTTCGGGGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCTGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                                   <  3:280689/136‑1 (MQ=255)
                                     aGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAcag                                                                                                  >  7:272310/1‑136 (MQ=255)
                                         gatgggcGGGCCATTGGCCAGGCGCCCGGCCTGTTCGGCGGCAACGGAAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGGGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                              <  4:258907/130‑1 (MQ=255)
                                             gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCt                                                                                          >  8:270061/1‑136 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCAACTCGTCGCGGAACAGCAGGCGCTTGGCCTg                                                                                   >  7:69111/1‑136 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCCGCGCCCCCCAACGCGTCGCGGAACACCCAGCGCGTGGCCTg                                                                                   >  2:121000/1‑136 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCGCTCGGCGCCCCCCAACTCGTCGAGGCACAGCCGGCGCGTGGCCTg                                                                                   >  7:123781/1‑136 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACACCGCGTCGCGGAACAGCAGGCGCTTGGCCTg                                                                                   >  5:238869/1‑136 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCCGCGGCCCGGGCGCGAACGGCGCCCCCACACGCGTCGAGGTACCCCCGGCGCGTGGccgg                                                                                   >  2:130105/1‑134 (MQ=255)
                                                         gCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTCCTCGTCGGCGCAGACTTCGCGGACAACCCCGGCGGCCAGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCg                                                                               <  2:184430/135‑1 (MQ=255)
                                                             ggCGCGCGGCCAGTTCGGCGCCACCGCCCGGCAACGGCGGGTGGGCGCAGACTTCGCTCCCCAACCGGGGGGCGCGGGGGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGTCGCTTGGCCTGCTCGCTGcc                                                                          <  4:151225/136‑1 (MQ=255)
                                                             ggCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                          <  8:96030/136‑1 (MQ=255)
                                                             ggCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTGCGCTCACCAACCCGGCGGCCTGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                          <  8:89768/136‑1 (MQ=255)
                                                               cgcCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCACGGCGCGATCGGCGCCCCACAAAACGTCGACGAACAACAGGCGCTTGGCCTGCACGCAGccc                                                                         >  1:264014/1‑134 (MQ=255)
                                                                 cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCAACTCGTCGAGGCACAGCAGGCGCTTGGCCTGCGCGCTGCCGATc                                                                      >  1:259812/1‑136 (MQ=255)
                                                                                  cAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCa                                                     <  8:6855/136‑1 (MQ=255)
                                                                                        caggcaAGTGCTCGTCGGGGCTGACTTCGGTGAGCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTGGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                               <  1:207962/136‑1 (MQ=255)
                                                                                        cagccaACGGCTCGGCGGCTCAGAGTTCGCTCACCACCCCGGCGGCCAGGGCCCGATCGCCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                               <  2:216477/136‑1 (MQ=255)
                                                                                              aCTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGc                                         <  6:44656/136‑1 (MQ=255)
                                                                                               cTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                        <  7:256638/136‑1 (MQ=255)
                                                                                               cTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                        <  2:263995/136‑1 (MQ=255)
                                                                                               cTGCTCGTCGGCGCAGACTTCGCTCACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                        <  7:155023/136‑1 (MQ=255)
                                                                                               cTGCTCGTCGGCGCAGACTGCGCGCACCACCCCGGCGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                        <  2:177289/136‑1 (MQ=255)
                                                                                                    cgtcgGCGCAGACTTCGCTGACCACCCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                                   <  2:227443/136‑1 (MQ=255)
                                                                                                     gtcgGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGTGCGGCGCCCAGCAACTCGTAGCGGTACAGCAGGCGGTTGGGCTGCTCGCTTCCGCTCCGCCGTGGAAGGGGCCAGCGGGCGCCGGCATCgggg                                  >  8:80892/1‑136 (MQ=255)
                                                                                                     gacgGCGCAGACTGCGCTGCCCACCTCGGCGGCCTGGGGGCGTCCGCCGCCCCACAACGCGTCGAGGAACAGCCGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                                  <  2:264407/134‑1 (MQ=255)
                                                                                                        ggCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                              >  5:189273/1‑73 (MQ=255)
                                                                                                        ggCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                              <  6:189273/73‑1 (MQ=255)
                                                                                                         gcgcATACTTCGGTGACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                              <  3:287955/136‑1 (MQ=255)
                                                                                                         gcgcAGACTGCGCGGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                              <  4:92901/136‑1 (MQ=255)
                                                                                                          cgcAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                             <  7:265103/136‑1 (MQ=255)
                                                                                                          cgcAGACTGCGCGGACCAACCCGGCGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                             <  5:290214/136‑1 (MQ=255)
                                                                                                          cccAGACTGCGCTCACCACCCCGGCGCCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                             <  7:98096/134‑1 (MQ=255)
                                                                                                             aGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGcc                          >  5:272614/1‑136 (MQ=255)
                                                                                                              gatttCGCTGACCACCCCGGCGGCCAGGGCGGGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc                         <  1:37573/133‑1 (MQ=255)
                                                                                                              gACTTCGCTCACCAACCGGGCGGCCAGGGCGCTTTGGGGGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc                         <  1:61754/136‑1 (MQ=255)
                                                                                                                      tGACCAACCCGGCGGCCAGGGCCCGATCGGCGCCCCACAACTCGTCGAGGAACCGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgt                 >  7:68172/1‑136 (MQ=255)
                                                                                                                        cccACCCGGGGGGCCCGGGCGCGAGGGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt               <  4:47759/135‑1 (MQ=255)
                                                                                                                        accaccCCGGGGGCCGGGGCGGGAGGGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGGTCGCTGCCGATCAGCCGTTGAAGGTTCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt               <  4:138671/136‑1 (MQ=255)
                                                                                                                        accaccCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt               <  5:62467/136‑1 (MQ=255)
                                                                                                                        aCCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt               <  5:65998/136‑1 (MQ=255)
                                                                                                                                   ggcCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTg    <  4:269450/136‑1 (MQ=255)
                                                                                                                                     ccAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGaa  >  8:224310/1‑136 (MQ=255)
                                                                                                                                     ccAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGaa  <  8:2873/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
CGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGAA  >  ACB143/4244993‑4245261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑