Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,720,070 C→T 8.2% D67N (GAC→AAC) ‡ sucD ← Succinate‑‑CoA ligase [ADP‑forming] subunitalpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,720,0700CT 8.2% 148.3 / 3.9 49D67N (GAC→AAC) ‡sucDSuccinate‑‑CoA ligase [ADP‑forming] subunitalpha
Reads supporting (aligned to +/- strand):  ref base C (21/24);  new base T (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01

TGACCTTGGCATCCAGCATGTCCAGGGTAGGAATGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGT  >  ACB143/4719938‑4720198
                                                                                                                                    |                                                                                                                                
tGACCTTGGCATCCAGCATGTCCAGGGTAGGAATGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGCAGCGTCAgc                                                                                                                               >  3:22031/1‑136 (MQ=255)
   ccTTGGCATCCAGCATGTCCAGGGTAGGAATGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGcc                                                                                                                            <  1:197118/136‑1 (MQ=255)
   ccTTGGCATCCAGCATGGCCCGGGTAGGAATGCCTTCGGTCATGCAGAGGATCAGCTTGATGCCGCCAAAGGAAGCTTCCCGTATCGAGTCTTTGCAGAAGGTAGCCGGTACGTAGATGACCGAAGCGTCAGCGcc                                                                                                                            <  2:253368/136‑1 (MQ=255)
          aTCCAGCATGTCCAGGGTAGGAATGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCt                                                                                                                     >  5:47756/1‑136 (MQ=255)
             cAGCATGTCCAGGGTAGGAATGCCTTCGGTGAGGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTcc                                                                                                                  <  8:167448/136‑1 (MQ=255)
                 aTGTCCAGGGTAGGAATGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACgg                                                                                                              >  7:137129/1‑136 (MQ=255)
                       aGGGTAGGAATGCCTTCGGTGATGCAGACGAGCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGACTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCt                                                                                                        <  2:222934/136‑1 (MQ=255)
                         ggTAGGAATGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTATATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTg                                                                                                      <  8:224102/136‑1 (MQ=255)
                                 tGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGtt                                                                                              >  8:5040/1‑136 (MQ=255)
                                 tGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGtt                                                                                              >  5:164356/1‑136 (MQ=255)
                                 tGCCTTCAGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGtt                                                                                              >  8:258403/1‑136 (MQ=255)
                                      tCGGTGATGCAGACTATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCAGGTCGCTTCCACGGCTTACTTGACGGTGTTGcaca                                                                                         >  6:218537/1‑136 (MQ=255)
                                      tCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAaca                                                                                         >  4:175299/1‑136 (MQ=255)
                                      tCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAaca                                                                                         >  2:296702/1‑136 (MQ=255)
                                        ggTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACAcc                                                                                       <  3:175299/136‑1 (MQ=255)
                                                aGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTACACGGCTTCCTTGACGGTGTTGAACACCGGCAGGcc                                                                               >  6:18499/1‑136 (MQ=255)
                                                     aTCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                          <  5:143777/136‑1 (MQ=255)
                                                     aTCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                          >  1:251744/1‑136 (MQ=255)
                                                      tCAGCTTTATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                          <  6:273942/135‑1 (MQ=255)
                                                      tCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                          <  8:52981/135‑1 (MQ=255)
                                                      tCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                          <  6:164356/135‑1 (MQ=255)
                                                                gccgccAAAGGCAGCTTCCAGGATCGCGGCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCa                                                               <  4:182125/136‑1 (MQ=255)
                                                                     cAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCtt                                                          <  8:17823/136‑1 (MQ=255)
                                                                       aaGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGc                                                        >  4:242620/1‑136 (MQ=255)
                                                                                       tCGAGTCTTTGCATAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACgccgcc                                        <  3:230370/136‑1 (MQ=255)
                                                                                             cTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCAt                                  >  3:2747/1‑136 (MQ=255)
                                                                                             cTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCAt                                  >  6:164755/1‑136 (MQ=255)
                                                                                              tttGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                 <  7:258403/136‑1 (MQ=255)
                                                                                                tGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                 <  2:295912/134‑1 (MQ=255)
                                                                                                tGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGGCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                 <  6:30986/134‑1 (MQ=255)
                                                                                                  cAGAACGGTGCTGGTACGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCAtttta                              <  3:101678/135‑2 (MQ=255)
                                                                                                   aGAACGGCGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGt                            <  4:114565/136‑1 (MQ=255)
                                                                                                   aGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGt                            <  6:47756/136‑1 (MQ=255)
                                                                                                        ggAGCTGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCg                        >  3:134217/1‑135 (MQ=255)
                                                                                                          aGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAg                     <  3:39252/136‑1 (MQ=255)
                                                                                                             cGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCCCCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGc                   >  4:296917/1‑135 (MQ=255)
                                                                                                             cGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGc                   >  8:179720/1‑135 (MQ=255)
                                                                                                               gTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTTAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAAt                <  7:177991/136‑1 (MQ=255)
                                                                                                                  cGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGc                          <  2:185538/123‑1 (MQ=255)
                                                                                                                  cGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGc                          >  1:185538/1‑123 (MQ=255)
                                                                                                                      gATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGt         <  3:229175/136‑1 (MQ=255)
                                                                                                                       aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGtt        >  6:141488/1‑136 (MQ=255)
                                                                                                                       aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGtt        >  8:54166/1‑136 (MQ=255)
                                                                                                                       aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCGTGGTGCCGTAGGCAATGGCCTGtt        >  4:228764/1‑136 (MQ=255)
                                                                                                                        tGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACATCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTc       <  8:153150/136‑1 (MQ=255)
                                                                                                                         gACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCCTCATGGTGCCGTAGGCAATGGCCTGTTCg      >  5:283514/1‑136 (MQ=255)
                                                                                                                            agAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGCCGTATGCAATGGCCTGTGCTGAg   >  2:72674/2‑136 (MQ=255)
                                                                                                                             gAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCTGAGt  <  5:18499/136‑1 (MQ=255)
                                                                                                                             gAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGt  <  1:93704/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                
TGACCTTGGCATCCAGCATGTCCAGGGTAGGAATGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGT  >  ACB143/4719938‑4720198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑