Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,720,083 T→A 7.8% E62D (GAA→GAT) ‡ sucD ← Succinate‑‑CoA ligase [ADP‑forming] subunitalpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,720,0830TA 7.8% 139.4 / 2.4 47E62D (GAA→GAT) ‡sucDSuccinate‑‑CoA ligase [ADP‑forming] subunitalpha
Reads supporting (aligned to +/- strand):  ref base T (19/24);  new base A (2/2);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

ATCCAGCATGTCCAGGGTAGGAATGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCC  >  ACB143/4719948‑4720217
                                                                                                                                       |                                                                                                                                      
aTCCAGCATGTCCAGGGTAGGAATGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCt                                                                                                                                        >  5:47756/1‑136 (MQ=255)
   cAGCATGTCCAGGGTAGGAATGCCTTCGGTGAGGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTcc                                                                                                                                     <  8:167448/136‑1 (MQ=255)
       aTGTCCAGGGTAGGAATGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACgg                                                                                                                                 >  7:137129/1‑136 (MQ=255)
             aGGGTAGGAATGCCTTCGGTGATGCAGACGAGCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGACTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCt                                                                                                                           <  2:222934/136‑1 (MQ=255)
               ggTAGGAATGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTATATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTg                                                                                                                         <  8:224102/136‑1 (MQ=255)
                       tGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGtt                                                                                                                 >  8:5040/1‑136 (MQ=255)
                       tGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGtt                                                                                                                 >  5:164356/1‑136 (MQ=255)
                       tGCCTTCAGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGtt                                                                                                                 >  8:258403/1‑136 (MQ=255)
                            tCGGTGATGCAGACTATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCAGGTCGCTTCCACGGCTTACTTGACGGTGTTGcaca                                                                                                            >  6:218537/1‑136 (MQ=255)
                            tCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAaca                                                                                                            >  2:296702/1‑136 (MQ=255)
                            tCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAaca                                                                                                            >  4:175299/1‑136 (MQ=255)
                              ggTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACAcc                                                                                                          <  3:175299/136‑1 (MQ=255)
                                      aGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTACACGGCTTCCTTGACGGTGTTGAACACCGGCAGGcc                                                                                                  >  6:18499/1‑136 (MQ=255)
                                           aTCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                                             >  1:251744/1‑136 (MQ=255)
                                           aTCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                                             <  5:143777/136‑1 (MQ=255)
                                            tCAGCTTTATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                                             <  6:273942/135‑1 (MQ=255)
                                            tCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                                             <  8:52981/135‑1 (MQ=255)
                                            tCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAggt                                                                                             <  6:164356/135‑1 (MQ=255)
                                                      gccgccAAAGGCAGCTTCCAGGATCGCGGCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCa                                                                                  <  4:182125/136‑1 (MQ=255)
                                                           cAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCtt                                                                             <  8:17823/136‑1 (MQ=255)
                                                             aaGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGc                                                                           >  4:242620/1‑136 (MQ=255)
                                                                             tCGAGTCTTTGCATAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACgccgcc                                                           <  3:230370/136‑1 (MQ=255)
                                                                                   cTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCAt                                                     >  3:2747/1‑136 (MQ=255)
                                                                                   cTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCAt                                                     >  6:164755/1‑136 (MQ=255)
                                                                                    tttGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                                    <  7:258403/136‑1 (MQ=255)
                                                                                      tGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                                    <  2:295912/134‑1 (MQ=255)
                                                                                      tGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGGCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                                    <  6:30986/134‑1 (MQ=255)
                                                                                        cAGAACGGTGCTGGTACGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCAtttta                                                 <  3:101678/135‑2 (MQ=255)
                                                                                         aGAACGGCGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGt                                               <  4:114565/136‑1 (MQ=255)
                                                                                         aGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGt                                               <  6:47756/136‑1 (MQ=255)
                                                                                              ggAGCTGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCg                                           >  3:134217/1‑135 (MQ=255)
                                                                                                aGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAg                                        <  3:39252/136‑1 (MQ=255)
                                                                                                   cGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCCCCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGc                                      >  4:296917/1‑135 (MQ=255)
                                                                                                   cGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGc                                      >  8:179720/1‑135 (MQ=255)
                                                                                                     gTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTTAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAAt                                   <  7:177991/136‑1 (MQ=255)
                                                                                                        cGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGc                                             >  1:185538/1‑123 (MQ=255)
                                                                                                        cGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGc                                             <  2:185538/123‑1 (MQ=255)
                                                                                                            gATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGt                            <  3:229175/136‑1 (MQ=255)
                                                                                                             aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGtt                           >  8:54166/1‑136 (MQ=255)
                                                                                                             aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGtt                           >  6:141488/1‑136 (MQ=255)
                                                                                                             aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCGTGGTGCCGTAGGCAATGGCCTGtt                           >  4:228764/1‑136 (MQ=255)
                                                                                                              tGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACATCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTc                          <  8:153150/136‑1 (MQ=255)
                                                                                                               gACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCCTCATGGTGCCGTAGGCAATGGCCTGTTCg                         >  5:283514/1‑136 (MQ=255)
                                                                                                                  agAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGCCGTATGCAATGGCCTGTGCTGAg                      >  2:72674/2‑136 (MQ=255)
                                                                                                                   gAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCTGAGt                     <  5:18499/136‑1 (MQ=255)
                                                                                                                   gAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGt                     <  1:93704/136‑1 (MQ=255)
                                                                                                                                 ggTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGc       <  2:158990/136‑1 (MQ=255)
                                                                                                                                      cTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGcc  <  2:73520/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
ATCCAGCATGTCCAGGGTAGGAATGCCTTCGGTGATGCAGACGATCAGCTTGATGCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCC  >  ACB143/4719948‑4720217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑