Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,720,137 A→G 8.6% G44G (GGT→GGC)  sucD ← Succinate‑‑CoA ligase [ADP‑forming] subunitalpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,720,1370AG 8.6% 130.8 / 4.9 47G44G (GGT→GGC) sucDSuccinate‑‑CoA ligase [ADP‑forming] subunitalpha
Reads supporting (aligned to +/- strand):  ref base A (16/26);  new base G (2/2);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

GCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCATTAC  >  ACB143/4720002‑4720271
                                                                                                                                       |                                                                                                                                      
gccgccAAAGGCAGCTTCCAGGATCGCGGCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCa                                                                                                                                        <  4:182125/136‑1 (MQ=255)
     cAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCtt                                                                                                                                   <  8:17823/136‑1 (MQ=255)
       aaGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGc                                                                                                                                 >  4:242620/1‑136 (MQ=255)
                       tCGAGTCTTTGCATAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACgccgcc                                                                                                                 <  3:230370/136‑1 (MQ=255)
                             cTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCAt                                                                                                           >  3:2747/1‑136 (MQ=255)
                             cTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCAt                                                                                                           >  6:164755/1‑136 (MQ=255)
                              tttGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                                                                                          <  7:258403/136‑1 (MQ=255)
                                tGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                                                                                          <  2:295912/134‑1 (MQ=255)
                                tGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGGCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATc                                                                                                          <  6:30986/134‑1 (MQ=255)
                                  cAGAACGGTGCTGGTACGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCAtttta                                                                                                       <  3:101678/135‑2 (MQ=255)
                                   aGAACGGCGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGt                                                                                                     <  4:114565/136‑1 (MQ=255)
                                   aGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGt                                                                                                     <  6:47756/136‑1 (MQ=255)
                                        ggAGCTGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCg                                                                                                 >  3:134217/1‑135 (MQ=255)
                                          aGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAg                                                                                              <  3:39252/136‑1 (MQ=255)
                                             cGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCCCCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGc                                                                                            >  4:296917/1‑135 (MQ=255)
                                             cGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGc                                                                                            >  8:179720/1‑135 (MQ=255)
                                               gTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTTAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAAt                                                                                         <  7:177991/136‑1 (MQ=255)
                                                  cGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGc                                                                                                   <  2:185538/123‑1 (MQ=255)
                                                  cGTAGATAACAGAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGc                                                                                                   >  1:185538/1‑123 (MQ=255)
                                                      gATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGt                                                                                  <  3:229175/136‑1 (MQ=255)
                                                       aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGtt                                                                                 >  6:141488/1‑136 (MQ=255)
                                                       aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGtt                                                                                 >  8:54166/1‑136 (MQ=255)
                                                       aTGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCGTGGTGCCGTAGGCAATGGCCTGtt                                                                                 >  4:228764/1‑136 (MQ=255)
                                                        tGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACATCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTc                                                                                <  8:153150/136‑1 (MQ=255)
                                                         gACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCCTCATGGTGCCGTAGGCAATGGCCTGTTCg                                                                               >  5:283514/1‑136 (MQ=255)
                                                            agAAGCGGTAGCGCCAGTGGCAGCAACGGCTTCACGCACGGTGTTGAACACCGGCAGGCCGAGGTGGGTGGTGCCGCCTTTACCTGGGGTTACGCCGCCAACCATTTTAGTGCCGTATGCAATGGCCTGTGCTGAg                                                                            >  2:72674/2‑136 (MQ=255)
                                                             gAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCTGAGt                                                                           <  5:18499/136‑1 (MQ=255)
                                                             gAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGt                                                                           <  1:93704/136‑1 (MQ=255)
                                                                           ggTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGc                                                             <  2:158990/136‑1 (MQ=255)
                                                                                cTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGcc                                                        <  2:73520/136‑1 (MQ=255)
                                                                                       ggCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAg                                                 <  2:251744/136‑1 (MQ=255)
                                                                                                 aCGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGa                                       <  8:160206/136‑1 (MQ=255)
                                                                                                          aaCACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTgg                              <  1:49332/136‑1 (MQ=255)
                                                                                                          aaCACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTgg                              >  7:96898/1‑136 (MQ=255)
                                                                                                           acacCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTgg                              <  7:263291/135‑1 (MQ=255)
                                                                                                               cGGCAGGCCCAGGTGGGTGTTGCCAACCTTGCCTGGGGTTACGCCGCCGAGCATCTTGGTGCCGTAGGCAATGGCCTGTGCGGAGTGGAAAGTACCCTGCGAGCCGGTGTAGCCCTGGCAGATGACTTTGGTATCt                         <  3:73962/136‑1 (MQ=255)
                                                                                                               cGGCAGGCCCAGGTGGGTGGTGCCACCGTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTTCGATCCGGTGAAGCCCTGGCAGATGACTTTGGTATCt                         <  4:99575/136‑1 (MQ=255)
                                                                                                                  cAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTa                      >  6:52647/1‑136 (MQ=255)
                                                                                                                    ggCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTAtt                    >  2:22841/1‑136 (MQ=255)
                                                                                                                     gCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTg                   <  4:296546/136‑1 (MQ=255)
                                                                                                                         aGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCa               <  3:144274/136‑1 (MQ=255)
                                                                                                                         aGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCa               <  5:99889/136‑1 (MQ=255)
                                                                                                                           gtgggtggTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAgg             <  6:134605/136‑1 (MQ=255)
                                                                                                                                   tgCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCAt     >  3:113130/1‑136 (MQ=255)
                                                                                                                                   tgCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCAt     >  3:272930/1‑136 (MQ=255)
                                                                                                                                   tgCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCAt     >  5:220081/1‑136 (MQ=255)
                                                                                                                                   tgCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCAt     >  1:223969/1‑136 (MQ=255)
                                                                                                                                      cACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCATTAc  <  7:143535/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GCCGCCAAAGGCAGCTTCCAGGATCGAGTCTTTGCAGAACGGAGCCGGTACGTAGATGACCGAAGCGTCAGCGCCGGTCGCTTCCACGGCTTCCTTGACGGTGTTGAACACCGGCAGGCCCAGGTGGGTGGTGCCACCCTTGCCTGGGGTTACGCCGCCGACCATCTTGGTGCCGTAGGCAATGGCCTGTTCGGAGTGGAAAGTACCCTGCGAGCCGGTGAAGCCCTGGCAGATGACTTTGGTATCTTTATTGATCAGGACGCTCATTAC  >  ACB143/4720002‑4720271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑