Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,037,507 C→T 5.8% H420Y (CAT→TAT)  CJ019_04731 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,037,5070CT 5.8% 296.6 / 4.5 86H420Y (CAT→TAT) CJ019_04731hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (43/38);  new base T (2/3);  total (45/41)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCCGAAACCTCATAGGGTATAACCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCGATCTCGCAAAATACCGCGCAGCGAAACAGTCTATC  >  ACB143/5037375‑5037641
                                                                                                                                    |                                                                                                                                      
ctcCGAAACCTCATAGGGTATAACCCTGAAGAGCACGAAAACGTAACAGTACTTGATTTGAGCGGCATCCCATTTGAACTGCTAAGCATTACACTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCAtt                                                                                                                                     <  4:152396/136‑1 (MQ=255)
 tcCGAAACCTCATAGGGTATAACCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCAt                                                                                                                                      <  5:211606/134‑1 (MQ=255)
  ccGAAACCTCATAGGGTATAACCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTa                                                                                                                                    >  3:218586/1‑135 (MQ=255)
  ccGAAACCTCATAGGGTATAACCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTa                                                                                                                                    >  4:103367/1‑135 (MQ=255)
  ccGAAACCTCATAGGGTATAACCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTa                                                                                                                                    >  6:254395/1‑135 (MQ=255)
   cGAAACCTCATAGGGTATAACCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCAtt                                                                                                                                     <  3:140647/133‑1 (MQ=255)
   cGAAACCTCATAGGGTATAACCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCAtt                                                                                                                                     <  7:176636/133‑1 (MQ=255)
   cGAAACCTCATAGGGTATAACCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCAtt                                                                                                                                     <  1:282713/133‑1 (MQ=255)
       aCCTCATAGGGTATAACCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGt                                                                                                                              <  8:64593/136‑1 (MQ=255)
       aCCTCATAGGGTATAACCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGt                                                                                                                              <  4:120036/136‑1 (MQ=255)
            aTAGGGTATAACCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTAc                                                                                                                          <  6:135787/135‑1 (MQ=255)
                 gTATAACCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAAcc                                                                                                                    <  3:65662/136‑1 (MQ=255)
                 gTATAACCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAAcc                                                                                                                    <  1:245057/136‑1 (MQ=255)
                 gTATAACCCAGAAGAGAACGCATACCTATCATTCGTTGATTTGATCGGCATCCGATTTGAAGTGCTAAGCATTACTGTATCGATGAAATCCCGTCTGATTTTTGAGGATGGGTACTATTACAAGGTCTACAGATcc                                                                                                                    <  4:39293/136‑1 (MQ=255)
                      aCCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCaaa                                                                                                                 >  7:9694/1‑134 (MQ=255)
                      aCCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCaaa                                                                                                                 >  1:249241/1‑134 (MQ=255)
                       cccTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAg                                                                                                              >  8:166322/1‑136 (MQ=255)
                       cccTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAg                                                                                                              >  1:28808/1‑136 (MQ=255)
                            aaGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTg                                                                                                          <  6:283997/135‑1 (MQ=255)
                            aaGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTg                                                                                                          <  3:121349/135‑1 (MQ=255)
                            aaGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTg                                                                                                          <  5:230458/135‑1 (MQ=255)
                             agagAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAATAATGGTgag                                                                                                        >  4:64358/1‑136 (MQ=255)
                             agagAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGt                                                                                                           >  6:230458/1‑133 (MQ=255)
                             agagAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTgag                                                                                                        <  2:249241/136‑1 (MQ=255)
                             agagAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGACTACAGAACCAAAAAGGGt                                                                                                           >  6:184787/1‑133 (MQ=255)
                               agaACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAATGGTgagg                                                                                                       >  1:288842/1‑134 (MQ=255)
                               agaACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTgaga                                                                                                       >  7:145142/1‑135 (MQ=255)
                               agaACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTgaga                                                                                                       >  3:81960/1‑135 (MQ=255)
                               agaACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTgaga                                                                                                       >  6:237644/1‑135 (MQ=255)
                               agaACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTgaga                                                                                                       >  3:29642/1‑135 (MQ=255)
                               agaACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTgaga                                                                                                       >  1:11465/1‑135 (MQ=255)
                               agaACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTgaga                                                                                                       >  2:133325/1‑135 (MQ=255)
                                    gCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACTATTACAAGGTCTACAGAAcc                                                                                                                    <  1:61909/117‑1 (MQ=255)
                                    gCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACTATTACAAGGTCTACAGAAcc                                                                                                                    <  1:57421/117‑1 (MQ=255)
                                    gCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACTATTACAAGGTCTACAGAAcc                                                                                                                    >  2:61909/1‑117 (MQ=255)
                                    gCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACTATTACAAGGTCTACAGAAcc                                                                                                                    >  2:57421/1‑117 (MQ=255)
                                    gCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTc                                                                                                  >  3:174936/1‑135 (MQ=255)
                                    gCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTc                                                                                                  >  7:165197/1‑135 (MQ=255)
                                      aaaCGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTcaa                                                                                                >  3:52644/1‑135 (MQ=255)
                                      aaaCGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTcaa                                                                                                >  3:130849/1‑135 (MQ=255)
                                        ccGTAACAGTCCTTGTTTTGAGCGGCTTCCCATTTGAAGTGCTAAGCATTACAGTCTCGTTGATTTCCCGTCTGATTTTTGCGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGTGTGAGACAGTcaacaa                                                                                             <  2:155548/135‑1 (MQ=255)
                                        aCGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTcaacaa                                                                                             <  1:101937/136‑1 (MQ=255)
                                        aCGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTcaacaa                                                                                             <  5:22749/136‑1 (MQ=255)
                                        aCGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTcaacaa                                                                                             <  2:215417/136‑1 (MQ=255)
                                        aCGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTcaacaa                                                                                             <  7:71462/136‑1 (MQ=255)
                                                gTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTc                                                                                     <  2:67016/136‑1 (MQ=255)
                                                gTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTc                                                                                     <  6:111531/136‑1 (MQ=255)
                                                       aTTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCt                                                                              >  1:176490/1‑136 (MQ=255)
                                                        tttGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTg                                                                             >  4:199933/1‑136 (MQ=255)
                                                              cGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGtt                                                                       >  4:60895/1‑136 (MQ=255)
                                                                  aTCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATg                                                                   <  5:254395/136‑1 (MQ=255)
                                                                     acATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATgaa                                                                 <  4:183876/134‑1 (MQ=255)
                                                                          tGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGc                                                            <  4:114122/135‑1 (MQ=255)
                                                                            aaGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTc                                                          <  8:143984/135‑1 (MQ=255)
                                                                            aaGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTc                                                          <  2:199256/135‑1 (MQ=255)
                                                                            aaGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTc                                                          <  3:40999/135‑1 (MQ=255)
                                                                            aaGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTc                                                          <  2:24459/135‑1 (MQ=255)
                                                                            aaGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTc                                                          <  6:128789/135‑1 (MQ=255)
                                                                              gTGCTAAGCATTACAGTATCGTTGTTTTCCCTTCTGATTTTTGATCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGCCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTcaca                                                       <  5:38064/136‑1 (MQ=255)
                                                                                 cTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAg                                                     <  3:227356/135‑1 (MQ=255)
                                                                                 cTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAg                                                     <  6:32461/135‑1 (MQ=255)
                                                                                    aGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATTGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATg                                                 >  4:68274/1‑136 (MQ=255)
                                                                                    aGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATg                                                 >  2:272732/1‑136 (MQ=255)
                                                                                    aGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATg                                                 >  2:245084/1‑136 (MQ=255)
                                                                                    aGCATTACAGTATAGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTAAAAGGTCTACAGAACCAAAAAGGGTGAGACAGTACAAAACGACGATGCAATCCTGCTAGTTTATGATGAAGATCACAAGTATg                                                 >  6:262596/1‑136 (MQ=255)
                                                                                            aGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGACCCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCAc                                          >  2:258655/1‑135 (MQ=255)
                                                                                            aGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCt                                                                                   <  8:45054/94‑1 (MQ=255)
                                                                                            aGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCt                                                                                   >  7:45054/1‑94 (MQ=255)
                                                                                            aGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCAc                                          >  1:227807/1‑135 (MQ=255)
                                                                                            aGTATCGTTGATTTACCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGTTGAGACAGTAAACAAAGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCAAAAGTATGTACCCAc                                          >  5:98485/1‑135 (MQ=255)
                                                                                                tCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCg                                     <  8:138754/136‑1 (MQ=255)
                                                                                                 cGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCGa                                    >  4:64949/1‑136 (MQ=255)
                                                                                                 cGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCGa                                    >  4:69132/1‑136 (MQ=255)
                                                                                                   ttGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGt                                                                                                   <  6:248567/71‑1 (MQ=255)
                                                                                                   ttGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGt                                                                                                   >  5:248567/1‑71 (MQ=255)
                                                                                                   ttGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCg                                     >  3:228986/1‑133 (MQ=255)
                                                                                                   ttGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCg                                     <  4:228986/133‑1 (MQ=255)
                                                                                                   ttGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCGAtc                                  >  5:165751/1‑136 (MQ=255)
                                                                                                   ttGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCGAtc                                  >  3:16113/1‑136 (MQ=255)
                                                                                                   ttGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCGAt                                   >  7:237280/1‑135 (MQ=255)
                                                                                                   ttGATTTACCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTTAGACAGTAAACAACGACGCTCCAATCCTCCTAGTTTATGAAGAACCTAACAAGTATTTACCCACTAGCGAtc                                  >  6:9003/1‑136 (MQ=255)
                                                                                                      aTTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCGATCTCg                               >  3:101211/1‑136 (MQ=255)
                                                                                                      aTTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCGATCTCg                               >  6:215092/1‑136 (MQ=255)
                                                                                                             gTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCGATCTCGCAAAATa                        <  1:145791/136‑1 (MQ=255)
                                                                                                                        gAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCGATCTCGCAAAATACCGCGCAGCg              >  5:178060/1‑135 (MQ=255)
                                                                                                                           cATGGCTACCATTAAAAGGTCTACAGAACCAAAAAGGGTGAGAGAGTCAAGAAAGACGCTCCTATCCTGCTAGTTTATGATGAATCTCACAAGTATGTACACACTAGCGATCTCGCAAAATACAGCGCAGCGAAAc          <  8:21295/136‑1 (MQ=255)
                                                                                                                                   ccATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCGATCTCGCAAAATACCGCGCAGCGAAACAGTCTATc  <  4:174936/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
CTCCGAAACCTCATAGGGTATAACCCTGAAGAGAACGCAAACGTAACAGTCCTTGATTTGAGCGGCATCCCATTTGAAGTGCTAAGCATTACAGTATCGTTGATTTCCCGTCTGATTTTTGAGCATGGCTACCATTACAAGGTCTACAGAACCAAAAAGGGTGAGACAGTCAACAACGACGCTCCTATCCTGCTAGTTTATGAAGAAGCTCACAAGTATGTACCCACTAGCGATCTCGCAAAATACCGCGCAGCGAAACAGTCTATC  >  ACB143/5037375‑5037641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑