| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 5,096,415 | C→G | 37.1% | intergenic (+28/‑162) | CJ019_04792 → / → CJ019_04793 | Putative pterin‑4‑alpha‑carbinolaminedehydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,096,415 | 0 | C | G | 37.1% | 24.8 / 11.7 | 32 | intergenic (+28/‑162) | CJ019_04792/CJ019_04793 | Putative pterin‑4‑alpha‑carbinolaminedehydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (9/11); new base G (9/2); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGT‑ATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGAC > ACB143/5096282‑5096546 | cACCGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGtt > 7:110147/1‑136 (MQ=255)cACCGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCtt > 1:63748/1‑136 (MQ=255) cGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCttttt < 5:162206/136‑1 (MQ=255) cGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCttttt < 2:63748/136‑1 (MQ=255) cGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCttttt < 3:149289/136‑1 (MQ=255) cGTGACCTGGTGGAGCCACTCGAGCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCttttt < 2:101609/136‑1 (MQ=255) gTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGtttttt > 5:10388/1‑136 (MQ=255) ccTGGTGGAGCGACTCGATCTAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCAGTGACAAGGTGGCGGAAACGGCTGATGGCCGGAAGTATACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATg < 3:244688/136‑1 (MQ=255) ccTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATg < 6:230318/136‑1 (MQ=255) tggtggAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTTGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCGATTATCAtatg > 4:197023/1‑133 (MQ=255) tggAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCa < 4:165511/136‑1 (MQ=255) aGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGGGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCatat < 1:292217/136‑1 (MQ=255) aTCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGAACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCGTTTTTCATGGGGCATATGTTCGCTgg > 6:166156/1‑135 (MQ=255) ccTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTGATGGGGCGTATTTGCGCTGTTGGCCCGc > 3:158227/1‑136 (MQ=255) ccTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTAGCCCg > 1:81269/1‑135 (MQ=255) ccGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAAcc > 4:194430/1‑136 (MQ=255) cGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACaaa > 8:226058/1‑136 (MQ=255) tcatcaTGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTAATGGGGCATATGTGCGCTGTTCGACCGCAAAACCCACAAACGAcc > 3:214464/1‑136 (MQ=255) tcatcaTGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTAATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGAcc > 3:59241/1‑136 (MQ=255) gtgCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGaaaaa > 3:85983/1‑135 (MQ=255) cacgcacTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAAc > 3:53436/1‑135 (MQ=255) gcacTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTAATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCCCCAAACGACCGGCAAAAAAACCaaa > 7:17875/1‑136 (MQ=255) gcacTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTGATGGGGCCTATTTGCGCGGTTGGCCCCCCAAACCCACAAACGACCGGGAAAAAAACCaaa > 3:178794/1‑136 (MQ=255) acacGGTGGCGTAACCGGCTGATGGCCGTAATTAAACACCAGATGGAAGGGGCCAGAGCCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTc < 5:166156/135‑1 (MQ=255) aCATGGTGGCGTAACCGGCGGACGGCCGTAAGAAAACACCTCACGCACGGGGCCATCGGCCCTTTTTTTCTTGGGGCATATGGGCGCTGTTCGCCCGAAAAACCCACAAACGACCGGGAAAAAAACCAAACTGTc < 1:278267/135‑1 (MQ=255) aGGTGGCGGAAACGGCGGAAGGCCGTAATTAACCACCAGATGGAAGGGGCCTGAGCCCCCTTTTTTCATGGGGAATATGTGCGCTGTGCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATcc < 2:59030/136‑1 (MQ=255) tGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTGATGGGGGATATGTGCGCGGTTGGCCCGCAAACCCCACAAACGCCCGGGAAAAAAACCAAACAGTGAAACAGACTTgtgtttgcag > 5:87137/1‑130 (MQ=255) aGTAACCACAAGATGGACGGTGCCTGAGGCCCGTTTTTTAATGGGGAATATGTCCGCTGTGCGCCCGCAAACCCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGT‑ATCCTGCCCGAGCtttt < 4:88109/136‑1 (MQ=255) gTAAACACCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTGATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGTAAAAAACCAAACTGTCATCCAGACTT‑TGTGATCCTGCCCGAGCttttt > 4:242698/1‑136 (MQ=255) aaggaaggGGCCAGAGGCCCGTTTTTTCATGGGGCATATGTGCGCTGCTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGT‑ATACTGCCCGAGCTTTTTGAAGCTACaa > 1:226275/1‑135 (MQ=255) ccgtggCCCCTTTTTTCATGGGGCATATGTGCGCTGCTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGT‑ATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGgcg < 2:226275/132‑1 (MQ=255) agGCCCGTTTTTTCATGGGGCATTTGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGT‑ATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAg < 4:85983/136‑1 (MQ=255) gctccTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGT‑ATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGAc < 2:234647/133‑1 (MQ=255) | CACCGTGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAAACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGT‑ATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGAC > ACB143/5096282‑5096546 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |