| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 504,981 | T→A | 25.4% | intergenic (+71/‑77) | trpC → / → CJ019_00453 | Indole‑3‑glycerol phosphate synthase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 504,981 | 0 | T | A | 25.4% | 39.0 / 2.9 | 30 | intergenic (+71/‑77) | trpC/CJ019_00453 | Indole‑3‑glycerol phosphate synthase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (7/9); new base A (5/3); total (18/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCTGGAATTACAGCGGCTGTTCTTCCCCGAGCAGGTGAAGAAGACTGTTCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑TACACGGGTAC‑GCGCCCTGTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGTGAAATCAACATCATAGGTGGATCAATGACCGATCAACCCCTGGCCCTGAGCGTCGAACAAGGCCTGCACGCCGAGCAGGAACT > ACB143/504846‑505116 | gcctggAATTACAGCGGCTGTTCTTCCCCGAGCAGGTGAAGAAGACTGTTCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑t < 7:53655/136‑1 (MQ=255)gcctggAATTACAGCGGCTGTTCTTCCCCGAGCAGGTGAAGAAGACTGTGCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑t < 4:142977/136‑1 (MQ=255) cAGCGGCTGTTCGTCCCCGAGCAGGTGAAGAAGACTGTACAGCAACTCGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑TACACGGGTAc‑g < 2:197747/136‑1 (MQ=255) aGCGGCTGTTCTTCCCCGAGCAGGTGAAGAAGACTGTTCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑TACACGGGTAc‑gc < 6:70989/136‑1 (MQ=255) aGCGGCTGTTCTTCCCCGAGCAGGTGAAGAAGACTGTTCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑TACACGGGTAc‑gc < 8:237307/136‑1 (MQ=255) aGCGGCTGTTCTTCCCCGAGCAGGTGAAGAAGACTGTTCAGCAACTGGACTGATCAAATGAAGCCGACGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑TACACGGGTAc‑gc < 3:121036/136‑1 (MQ=255) gCGGCTGTTCTTCCCCGAGCAGGTGAAGAAGACTGTTCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑TACACGGGTAc‑gcg < 2:196494/136‑1 (MQ=255) gCGGCTGTTCTTCCCCGAGCAGGTGAAGAAGACTGTTCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑TACACGGGTAa‑gcg > 7:131287/1‑136 (MQ=255) ttcttcCCCGAGCAGGTGAAGAAGACTGTTCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑TACACGGGTAC‑GCGCCCTGt < 7:244987/135‑1 (MQ=255) aGGTGAAGAAGACTGTTCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTAGCGGGCAAGCCCGCTCC‑AACACGGGTAC‑GCCCCCTGTCGTAGGAGCCGGtt > 2:96496/1‑136 (MQ=255) aGGTGAAGAAGACTGTTCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCATCATAGCCGGCAAACACCCTCCAAACAC‑GGTAC‑GACCCCCGTGGTATGCGCGGGtg > 7:125710/1‑135 (MQ=255) tGTTCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTAGCGGGCCAGCCCCCTCC‑GACACCGGGTC‑GCGCCCGGGGGGAGGGGCGGGGTTTCCCCCGCagag > 1:189177/1‑136 (MQ=255) ttCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑TACACGGGTAc‑gcg < 1:240783/101‑1 (MQ=255) ttCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑TACACGGGTAc‑gcg > 2:240783/1‑101 (MQ=255) ttCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTAGCCGGCCACCCCCCTCC‑GACACGGGGAC‑GCGCCCTGTGGTAGGAGCCGGGTTTCCCCCCAagacgg > 7:88385/1‑133 (MQ=255) tCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCCGGCAAGCCCGCGCC‑GACACGGGTAC‑GCCCCCGGTAGGAGGCGCGGGTTTACCCGCGAAGAGGcc > 1:10505/1‑136 (MQ=255) tCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTAGCGGGCCAACCCCCGCC‑GACACCGGGAC‑GCGCCCGGGGGTAGGAGCGGGGTTAACCCCGCAGAGGcc > 5:51152/1‑136 (MQ=255) aGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTTTACCTGTAATGGCCTCATGGCCGGGCAGCCCCCCCC‑AAAACCGGTGC‑GCCCCCAGGGGTAGGTGCGGGTTTAACCGCCAAGAGGcccg > 4:169897/1‑134 (MQ=255) aGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTGC‑TACACGGGTAC‑GCGCCCGGTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCAg > 5:278458/1‑136 (MQ=255) aGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑TACACGGGTAC‑GCGCCCTGTCGTa < 5:175077/108‑1 (MQ=255) aGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑TACACGGGTAC‑GCGCCCTGTCGTa > 6:175077/1‑108 (MQ=255) aGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTAGCGGGCCAGCCCGCGCC‑AAAACCGGTAA‑GCGCCCGGGCGTAGGAGCGGGTTTACCCGCGAATAGGCCAg > 2:168797/1‑136 (MQ=38) cTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTGGCGGGCAAACCCCCGCC‑TACACCGGTAA‑CCGCCCGGTGGTTGGGGCGGGTTTAACCCCGAAGAGGGCACTGAAATaaa > 3:65255/1‑135 (MQ=255) cTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTAGCGGGCAATCCCCCGCC‑AAAACGGGTAA‑GCGCCCGGTGGTTGGCGCGGGTTTTCCCCCGAAGAGGGCAGTGAAATCaa > 3:69391/1‑135 (MQ=255) cTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCATAGCCGGCAATCCCCCACC‑GACACCGGTAC‑GCGCCCGGTGGTAGGAGCCGGTTTACCCGCCAAGAGGCCAGTGAAAACaa > 3:199176/1‑135 (MQ=255) cAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑GTCACGGGTTC‑GGGCGCTGTCGGTGGAGCGGGTTTTCCCGCGAAGGGGGCAGTGAAATCATcctcat > 5:144673/1‑136 (MQ=255) aacttaaGCCGGGTTTTTTGCGGGCTTTTTTGTACCTTAAATGGCCTCTTCGGGGGAAAGCCCGCTCC‑AACAGGGGTAC‑GCGCCCTGTCGTCGGCGGGGTTTTACCCGCGAAGAGGCCAGTGAAATCAACATCATa < 6:58428/131‑1 (MQ=255) tttccgggCTTTTTTGTACATTTAAGGCCCCCTTCGCGGGCAAGCCCGCTAC‑AACACGGGAAC‑GCGCCGTGTCGATGGCGGGGGTTTACCCGCGAAGAGGCCAGTGAAATCAACATCATAGGTGGATCAATGAcc < 2:79537/129‑1 (MQ=255) agggCCTCTGCGCGGCCACCCCCGAT‑C‑AACCCGGGTACGGCGCCCTGTCGTTGTGGGGGTTTAACCCGCTAAGAGGCCAGTGAAATCAACATCATAGGTGGATCAATGACCGATCAACCCCTGGCCCTGAGCGTCg < 1:168797/134‑1 (MQ=255) ttCGCGGGCAAGCCCGCTCC‑TACACGGGTAC‑GCGCCCTGTCGt < 7:220880/43‑1 (MQ=255) ttCGCGGGCAAGCCCGCTCC‑TACACGGGTAC‑GCGCCCTGTCGt > 8:220880/1‑43 (MQ=255) cAAGCCCGCTCC‑TACACGGGTAC‑GCGCCCTGTGGTATGAGCGGGGTTACCCGCGAAGAGGCCAGTGAAATCAAAAAAATATGTGGATCAATTACCCATCAACCCCTGGCCCTGAGCGGCGAACAATGCCTGCAccc > 6:215297/1‑134 (MQ=37) tACCCGGGTAC‑GCGCCCTGTCGTAGGAGCGGGGTTACCCGCGAAGAGGCCAGGGAAATCAACATCATAGGTGGATCAATGACCGATCACCCCCTGGCCCTGAGCGTCGAACAAGGCCTGCACGCCGAGCAGGAAct > 7:80173/1‑136 (MQ=255) tACACGGGTAC‑GCGCCCTGTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGTGAAATCAACATCATAGGTGGATCAATGACCGATCAACCCCTGGCCCTGAg > 4:62953/1‑103 (MQ=255) tACACGGGTAC‑GCGCCCTGTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGTGAAATCAACATCATAGGTGGATCAATGACCGATCAACCCCTGGCCCTGAg < 3:62953/103‑1 (MQ=255) | GCCTGGAATTACAGCGGCTGTTCTTCCCCGAGCAGGTGAAGAAGACTGTTCAGCAACTGGACTGATCAAATGAAGCCGGCGTTTTTGCCGGCTTTTTTGTACCTGTAATGGCCTCTTCGCGGGCAAGCCCGCTCC‑TACACGGGTAC‑GCGCCCTGTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGTGAAATCAACATCATAGGTGGATCAATGACCGATCAACCCCTGGCCCTGAGCGTCGAACAAGGCCTGCACGCCGAGCAGGAACT > ACB143/504846‑505116 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |