Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 627,228 T→G 33.9% S162A (TCG→GCG)  acoR_1 → Acetoin catabolism regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143627,2280TG33.9% 18.8 / 2.4 39S162A (TCG→GCG) acoR_1Acetoin catabolism regulatory protein
Reads supporting (aligned to +/- strand):  ref base T (12/13);  new base G (7/7);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGAAGATGATC  >  ACB143/627093‑627359
                                                                                                                                       |                                                                                                                                   
gggCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGCCCTGCt                                                                                                                                     >  7:22269/1‑136 (MQ=255)
      tgGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGGCGCGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCGGACCTGCTCGGCCa                                                                                                                               <  2:20443/136‑1 (MQ=255)
         cTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCTGGCCGGCTCGGCCAccc                                                                                                                            >  1:153002/1‑136 (MQ=255)
               gcTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCTGTCCGGCTCGGGCCGCCCGgggt                                                                                                                      >  7:25912/1‑136 (MQ=255)
               gcTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGAGCTGACCCTCCATCAGCACGAGCCCTTCCGTGGCCGTCCCCACCGGCGGACCGACGCGGCACGCCCgggtg                                                                                                                      >  8:199770/1‑133 (MQ=255)
                               cgcgcgAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCTGGCCGGCGCGGGCCGCCCCGGGTTGGGCCCGCATggggc                                                                                                      >  3:122572/1‑133 (MQ=255)
                                gcgcgAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTGGCACCGGGCGGGGCTGGGCGGGCCGGCCGGGGTTGGACCCGCATgggggc                                                                                                     >  3:110230/1‑132 (MQ=255)
                                gcgcgAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTGACCCCCGGCTGACCAGCGAGGGCCGCCCGGTGTTGGGCCCCCATgggggc                                                                                                     >  8:249302/1‑132 (MQ=255)
                                gcgcgAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCAGCTCGGCCAGCCCGGGGTTCGACCCGCATTGCgcc                                                                                                     >  6:243876/1‑134 (MQ=255)
                                    gcgggCACAAATGCCAGCGCAACATGTCTGGCCGAGCGCCAGGCGCTGACCATCCATCACACCCAGCACTTCGGTGGCCGGCACACCGGGCTGACCTGCTCGGCCAGCCGGTTGTTCGACCCGCATGGCGACctgc                                                                                                 <  2:5824/134‑1 (MQ=255)
                                    gAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCAAACCCGGCAGACCTGCGCGGCCAGCCCGGTGTTAGACCCGCCTGGCCACcagc                                                                                                 >  6:266033/1‑136 (MQ=255)
                                     agaccgccAATGCCAGCGGCACTTGGCTGGGCGAGCGCCCGGCGCTTACCATCCATCACACCGCGCACTTCCGGGGCCGCCACCCCGGGCTGACCGGCGCGGCCAGCCCGGTGTTCGACCCGCATGGCGACctgct                                                                                                <  6:216239/130‑1 (MQ=255)
                                     aGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCTGGCCTGGGCGGCCCGCCCGGTGTTCGTCCCGCATGGCGACcggcg                                                                                                >  7:59509/1‑135 (MQ=255)
                                      cagCACCAATGGCTTCGGCAAATGTCTGGGCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGGAACACGGGGCTGACCTGCGCGGCCAGCCCGGTGTTCGACCCGCATGGCGACctgc                                                                                                 <  2:22324/132‑1 (MQ=255)
                                       ggCACAAATGGCAGCGCCACTTGTCTGGCCGAGCGCCAGGCGCTGACCATCCATCAGACCGACCCCTCCCGGGGCCGCCACACCGGGCTGACCTGCGCGGCCAGCCGGGTGTTCGACCCGCATGGCGACCTGCTgg                                                                                              <  2:278839/136‑1 (MQ=255)
                                          cccAATGGCAGCGGCACAGGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGACCGAGCCATTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCgg                                                                                           <  6:286547/135‑1 (MQ=255)
                                          acaaaTGGCATCGGCACAGGTGGGGGCGAGCGCCAGGCGGTGACAATCCTTCAGAACGAGCACTCCCGGGGCCGGCACCCCGGGCTGACGTGCTCGGCCAGCCGGGTGTTCGACCCGCATGGCGACCTGCTGGCgg                                                                                           <  4:25796/133‑1 (MQ=255)
                                          acaaaTGGCATCGCAACTGGCCGGGGCGAGCGCAAGGCGCTAACAAACCAAAACACCCCCCACTTCCGTGGCCGCCACACGGGGCTGACCTGCGCGCCCCGCCGGGTCTTCGACCCGCATGGCGACCTGCTGGCgg                                                                                           <  6:170124/133‑1 (MQ=255)
                                          aCCAATGGCAGCGCCACATGTCTGGGCGCGCGCCAGGCGCTGACCATCCATCAGACCGACCACTTCCGGGGCCGTCACACCGGGCTGACCTGCGCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCgg                                                                                           <  2:261686/136‑1 (MQ=255)
                                               tGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGTCCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGGCCTGCTGGCGGTGCTc                                                                                      >  7:28897/1‑136 (MQ=255)
                                                     cGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCGGACCTGCTCGGCCAGCCCGGTGTTCGCCCCGCATGGCGCCCAGCTGGCGGTGCTCGACgtg                                                                                >  2:161269/1‑136 (MQ=255)
                                                      ggCACATGGCTGGTCGAGCGCCCGGCGCTGACCAGCCCTCCCACCGAGCACTACCGGGGCCGTCACACCGGGCTGACCTGCTGGGCCCGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACgtgt                                                                               <  4:90256/136‑1 (MQ=255)
                                                         acaTGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCCCACCCGGCGGCCCGGCTCGGCCCGCCCCGGGTTGGACCCGCAAGGCGCCCGGCTGGCGGTGCTCGCCgcggggt                                                                            >  5:216239/1‑130 (MQ=255)
                                                         acaTGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCTGACCTGCTCGGGCAGCCCGGTGTTGGACCCGCCGGGCGACCTGCTGGCGGTGCGCGACGTGGccg                                                                            >  4:252732/1‑135 (MQ=255)
                                                           aTGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCctct                                                                          <  7:137877/136‑1 (MQ=255)
                                                                     gAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCCCACCCGGGTGGCCGGGGCGGGCCGCCCGGGGTTCGCCCCGCCTGGCGCCCTGCTGGCGGTGGGGGCCGGGGCGGCTGCGCGCCCCg                                                                >  4:187158/1‑136 (MQ=255)
                                                                         gcaaGGCGCTGACCATCCATCACAACGAGCACTGCCGTGGCCGTCACACCGGGCTGACCTGCGCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGt                                                            <  1:195985/133‑1 (MQ=255)
                                                                          ccAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTGACACCCGGCGGCCCTGCTCGGCCCGCCCCGGGGTCGGCCCCCCTGGCGCCCGGCGGGCGGTGGTCGACGTGTCCTCTGCGCGcccccgcgtc                                                           >  5:212081/1‑130 (MQ=255)
                                                                              gggcTGACCATCCATCAGAAGGAGCACTTCCGGGGCCGTCACACCGGGCTGACCTGCGCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATcgc                                                       <  1:161269/134‑1 (MQ=255)
                                                                              gcgcTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACCCCGGGCTGACCTGCTCGGCCAGCCGGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATcgc                                                       <  4:25686/136‑1 (MQ=255)
                                                                               cgcTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCCGGCTGACCTGCTCGGCCAGCCCGGGGGTCGACCCCCATGGCGCCCTGCTGGCGGTGCTCGACGGGTCCTCGGCGCGCCCCGACGGATcgcg                                                      >  1:212278/1‑136 (MQ=255)
                                                                                    aCCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCaa                                                  <  2:171646/135‑1 (MQ=255)
                                                                                           atcAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTTTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGtt                                          <  2:13817/136‑1 (MQ=255)
                                                                                               gaccGAGCACTTCCGGGGCCGTCACACGGGGGTGACCTGCTCGGCCAGCCGGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCcac                                      <  7:131857/133‑1 (MQ=255)
                                                                                                 aCGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCCGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTTTCGCGGCAAAGACAGTTCCacac                                    >  6:207305/1‑136 (MQ=255)
                                                                                                      cACTTCCGTGGCCGTCACAACGCGCTGACCTGCTCGGCCCGCCCCGGGTTCGACCCCCATGGCGCCCGGCTGGCGGGGCTCGACGTGTCCACAGCGCGCCCCCACGGATCGCGGCAAAGCCCGTTCCACACCCTgg                               >  8:77871/1‑136 (MQ=255)
                                                                                                                           ggggggACCGGCTCGGCCTGCCGGTTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGa          <  4:110230/131‑1 (MQ=255)
                                                                                                                               tGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGAAgat      >  4:33064/1‑136 (MQ=255)
                                                                                                                                   cTGGTCGGCCAGCGGGTTGATCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCTTTGGTCAACCTGTCGGCGAAGATGATc  <  8:172868/136‑1 (MQ=255)
                                                                                                                                   cTGCTCGGCCTGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGAAGATGATc  <  1:31421/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
GGGCTGTGGCTGGGCGCTGACTGGAGCGAGGCGCGCGAGGGCACCAATGGCATCGGCACATGTCTGGTCGAGCGCCAGGCGCTGACCATCCATCAGAACGAGCACTTCCGTGGCCGTCACACCGGGCTGACCTGCTCGGCCAGCCCGGTGTTCGACCCGCATGGCGACCTGCTGGCGGTGCTCGACGTGTCCTCTGCGCGCCCCGACGTATCGCGGCAAAGCCAGTTCCACACCATGGCGTTGGTCAACCTGTCGGCGAAGATGATC  >  ACB143/627093‑627359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑