Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,693,183 T→G 42.5% R238R (CGT→CGG)  pyrC' → Dihydroorotase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,693,1830TG42.5% 12.2 / 11.5 44R238R (CGT→CGG) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (9/16);  new base G (2/17);  total (11/33)
Fisher's exact test for biased strand distribution p-value = 8.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTGCG  >  ACB143/5693057‑5693317
                                                                                                                               |                                                                                                                                      
cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTTCCGCCCGATCACCCGCCCCCGTGGCGCGCgg                                                                                                                               >  2:5143/1‑136 (MQ=255)
      cAGCTTCCTGGGCCTGCCGGGCATCGCGGAGAGCGCCGAACCCGGGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGGGGGCGTGCGCACTTCAGCCAGACCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                                         <  1:298654/136‑1 (MQ=255)
          ttCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCCGATCACCCGCGCCCGGGGCGCGCGGCGGCTCgccc                                                                                                                     >  3:37995/1‑136 (MQ=255)
            ccTCGGCGTGCCGGCCCTCCGGGAGCGCCCCGAACCCGGGGCCCGGGCGCGCAACCTGG‑TGCTGGTGGAACTCTGCGGGGTGCTGGCGCACTTCAGCCAGACCCCCCGCGCCCGTGGCGCGCGGCTGATCgcccag                                                                                                                   <  4:229993/136‑1 (MQ=255)
            ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCATAAGCCCACTAACCACCGCCCCGGGCGCCCGGCAGCTAgcccac                                                                                                                   >  2:155245/1‑135 (MQ=255)
              gcgccgtGCGGGGCATCCCGGAGAGCGCCTAAACCGTGGCCCGGGCGCGAAACCTGC‑TGCTGGGGCAACTGAGCGGCGTGCGTGCGCACTTCAGCCACACCCCCAGCGCCCGTGGCGCGCGGCTGATCgcccaggc                                                                                                                 <  2:56411/130‑1 (MQ=255)
              gcGGCCTGCCGGGCATCCCGGAGAGCGCCGAACCGGGGGCCCGGGCGCGCAACCTGC‑TGCTGGGGGAACACCGCGGCGGGGGGGCGCACTACAGCCAGAACCCCCGCGCCCGGGGCGCGCGGCTGATCgcccaggc                                                                                                                 <  8:31371/135‑1 (MQ=255)
                ggccgcccGGGCATCCCGGAGAGCGCCTAAACCGGGGCCCGGGCGCGCACCCGGC‑TGCGGGTGGAACCGCGCGGCGGGGGTGCGCAATCCCGCCAGAACACCCGCGCCCGGGGCGCGCGGCTGATCgcccaggc                                                                                                                 <  4:82272/128‑1 (MQ=255)
                ggCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGGGGCCCTGGCGCGCAACGTGC‑GGCTGGTGGAACAGAGCGGGGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccaggc                                                                                                                 <  5:195098/134‑1 (MQ=255)
                  ccggCCGGCCATCCCGGACCGCCCCGAACCGGGGGCCCTGGCGCGCAACCTGC‑TGCTGGGGTAACAGCGCGGGGGGCGGGCCCACTGCCGCCCGAGCCCCCGCGCCCGGGGCGCGCGGCTGATCgcccaggcccag                                                                                                             <  6:199519/133‑1 (MQ=255)
                        gggctgcccGGAGAGCGCCTAAACGGGGGCGGGGGCGCGCACCGTGG‑TGCTGGGGGAACCGCGGGGCGGGCGGGCGCAATCCCCCCCGACCACCCGGGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                                                       <  2:107956/130‑1 (MQ=255)
                                cGGTGAGCGCCTAAACCGGGGCCCTGGCGCGCACCCTGC‑GGCGGGTGGAACAGAGGGGCGTGCGGGCGCACTTCAGCCCGAGCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGcc                                                                                               <  5:69698/136‑1 (MQ=255)
                                 ggAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCg                                                                                              <  7:255648/136‑1 (MQ=255)
                                      ggccgtaaaCGGGGGCCCTGGCGCG‑AAACTGCGTGGTGGTGCACCCGCGGGGCGGGCGGGGGCACTACAGCCAGACCACCCGCGCCGTGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                         <  2:266736/130‑1 (MQ=255)
                                      gcgcCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                         <  5:262354/136‑1 (MQ=255)
                                      gcgcCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCGGGTGGAACAGAGCGGCGGGCGGGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                         <  7:52109/136‑1 (MQ=255)
                                      gcgcCGAAACCGGGGCCCTGGCGCGCAACCTGC‑TGCTGGGGGACCAGAGCGGCGTGCGTGCGCACTTCAGCCAGAGCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                         <  4:223893/136‑1 (MQ=255)
                                                gggggCCGTGGCGCGCAACCTGC‑GGGTGGGGCAACCGCGGGGGGGGGGGGCGCACTGCAGCCAGATCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  3:201062/133‑1 (MQ=255)
                                                ggTGGCCCTGGCGCGAAACGTGC‑TGCTGGTGTAACAGAGCGGCGTGCGTGCGAACTACCGCCATATCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  5:251366/135‑1 (MQ=255)
                                                cggggCCGTGGCGCGCAACCTGG‑TGCTGGTGGAACCGCGCGGCGGGCGGGCGCAATTCACCCAGACCCCCCGCGCGCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  4:18293/133‑1 (MQ=255)
                                                cggggCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  6:274609/133‑1 (MQ=255)
                                                cGTGGCCCTGGCGCGCACCCTGC‑TGCGGGGTGAACAGTGCGGCGGGGGTGCGAACTTCAGCCAGATCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                               <  2:90441/136‑1 (MQ=255)
                                                     cccTGGCGCGCAACCTGC‑TGCTGGGGTAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCt                                                                          <  6:62708/136‑1 (MQ=255)
                                                            gcgcAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGCTCGCCCCGGCCCCGGCCGTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTAcccg                                                                   >  1:176015/1‑134 (MQ=255)
                                                             cgcAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCATAAGCCCGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCCGGCCGTGGGCCTGCCGGTAACCGCCGCTGTAGCGCGGTTCCAGc                                                                  >  7:21306/1‑136 (MQ=255)
                                                              gcAACCTGC‑GGCTGGGGGAACAGAGGGGCGTGGGTGCGCACTTCAGCCAGATCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCt                                                                 <  7:78405/136‑1 (MQ=255)
                                                                                     gagCGGCGTGCGTGCGCACTTCAGCCACACCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTg                                           <  1:37752/136‑1 (MQ=255)
                                                                                     gagCGGCGTGCGGGCGAACGGCACCCACATCACCCCCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCGGTACCAGCTGATCCTCACGGACGAGTCGCTg                                           <  6:84586/136‑1 (MQ=255)
                                                                                       gcggcgTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGCTCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACCGACGAGTCGCTgcg                                         >  7:78720/1‑136 (MQ=255)
                                                                                            gtgcgtgcGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  8:215020/136‑1 (MQ=255)
                                                                                            gggggtgcgAACTTCAGCCATAGCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  2:22748/132‑1 (MQ=255)
                                                                                            gggcgtgcgAAGTTCGCCCAGATCCCCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  4:294955/134‑1 (MQ=255)
                                                                                            gggcgggcgCACTCCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  2:70034/134‑1 (MQ=255)
                                                                                            gggcgggccCACTTCCCCCCGACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                    <  1:139922/134‑1 (MQ=255)
                                                                                              gcgtgcgCACTTCAGCCAGATAACCAGCGCCCGTGGCGCGCGGCTGATAGCCCAGGCCCCGGCCTTGGGCCTGCCGGTAACCGCCGAGGTATCGCTGTACCAGCTGATCCTCACGGACTAGTCGCTGCGCGAGtta                                  >  2:81688/1‑135 (MQ=255)
                                                                                              gcgtgcgCACTTCAGCCAGACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc                                  <  6:203227/136‑1 (MQ=255)
                                                                                              gcgtgcgCACATCCGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCGTGGGCCTTCCGGGAACCGCCGCTGTAGCGCTGTACCCGCTGATCCTCACGGACGCGTCGCTGCGCGAGTtc                                  >  7:150129/1‑136 (MQ=255)
                                                                                                gggcgcACTTCAGCAAGATCACCAGCGCCCTGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtctc                                <  1:259860/134‑1 (MQ=255)
                                                                                                 tgcgCACTTCAGCCAGATCACCAGCGCCCGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                               <  8:25409/136‑1 (MQ=255)
                                                                                                 ggcgcCCGTCAGCCAGAACACCCGCGCCCGTGGCCCGCGGCTGATCGCCCAGGCCCAGTCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                               <  6:129159/135‑1 (MQ=255)
                                                                                                                gATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTACCCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTg                >  3:184818/1‑136 (MQ=255)
                                                                                                                   cACCCGGGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg             <  3:70107/136‑1 (MQ=255)
                                                                                                                              gTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTgcg  >  3:73683/1‑136 (MQ=255)
                                                                                                                              gTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTgcg  >  1:7767/1‑136 (MQ=255)
                                                                                                                               |                                                                                                                                      
CATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTGCG  >  ACB143/5693057‑5693317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑