Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 44,301 (T)9→8 intergenic (+132/‑359) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB14344,2930T.98.0% 233.7 / ‑3.0 51intergenic (+124/‑367)oprD_1/czcR_2Porin D/Transcriptional activator protein CzcR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (20/30);  minor base G (1/0);  total (21/30)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAACGAAGC  >  ACB143/44169‑44420
                                                                                                                            |                                                                                                                               
aTTAGCTAACTGGGCCAGTTAACCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTCGCTTAAATGCACCTTTGGTAAAACGTGCATATTTGGCGCCCATTGGGCGCCtatttattgac                                                                                                                       >  6:348696/1‑132 (MQ=255)
        aCTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCa                                                                                                             <  7:187291/136‑1 (MQ=255)
         cTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCa                                                                                                             <  3:179596/135‑1 (MQ=255)
           gggCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAg                                                                                                           >  6:279083/1‑135 (MQ=255)
            ggCCAGTTATCCATTGTCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAg                                                                                                           <  8:195436/134‑1 (MQ=255)
               cAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGc                                                                                                      <  2:129241/136‑1 (MQ=255)
                 gTTATCCATTGGCGGTTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTTAGTTGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCATATAATTAAAGTAGGCGt                                                                                                    >  6:192644/1‑136 (MQ=255)
                  ttATCCATTGGCGGGTCCTGTTAGTTAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGtt                                                                                                   <  1:368239/136‑1 (MQ=255)
                   tATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGtt                                                                                                   <  4:378759/135‑1 (MQ=255)
                       cATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttt                                                                                                                               >  8:283677/1‑104 (MQ=255)
                       cATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttt                                                                                                                               <  7:283677/104‑1 (MQ=255)
                               ggTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCGTTTTTTTTG‑CTAAAATTCAAGTAGGCGTTGGATTGttagca                                                                                       >  5:242653/1‑130 (MQ=255)
                               ggTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTAAAGTAGGCGTTCGATTGTAcgcat                                                                                      >  5:141744/1‑136 (MQ=255)
                                gTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgcatc                                                                                     <  2:241415/136‑1 (MQ=255)
                                         gTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                             <  1:177085/135‑1 (MQ=255)
                                          tAAAACGAGCATTACGCATTCACCTTGAGAGGCTTAAGTTCTCCTTTGGTATAAGGTGGATTTTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                             <  3:225981/134‑1 (MQ=255)
                                             aaCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTg                                                                          <  4:190272/134‑1 (MQ=255)
                                              aCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGGTATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAg                                                                       <  6:173014/136‑1 (MQ=255)
                                              aCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑GTTTTTTTGCCTAAAATTTAAGTAGGGCTTGGATTGTACGACTCGCATGCCGGTGAAg                                                                       >  6:36531/1‑136 (MQ=255)
                                                gAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGtt                                                                     <  7:244069/136‑1 (MQ=255)
                                                gAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGtt                                                                     <  6:86489/136‑1 (MQ=255)
                                                     ttACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                                <  1:132173/136‑1 (MQ=255)
                                                     ttACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                                <  2:146722/136‑1 (MQ=255)
                                                      tACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCCTTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCTATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                                <  3:235820/135‑1 (MQ=255)
                                                           aTTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTacac                                                          >  5:308235/1‑136 (MQ=255)
                                                               aCCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCaa                                                       <  5:279083/135‑1 (MQ=255)
                                                                ccTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGAGCC‑TTTTTTATGCCTAAAATTCAAGTAGGAGTTCGATTTTACGCATCCCATTCCCGTGAAGTTCATGACTACACCa                                                        >  1:99767/1‑133 (MQ=255)
                                                                    gAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                                  <  6:141744/135‑1 (MQ=255)
                                                                     aGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTAATGACTACACCAAAGACCAg                                                >  5:330338/1‑136 (MQ=255)
                                                                      gTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGt                                               >  5:226373/1‑136 (MQ=255)
                                                                        ggCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAAATAAAGTAGGCGTTCGATTGTACGCATAGCATGCCGGTGAAGTTTATTACTAAACCAATGACCAGtct                                             >  6:310348/1‑136 (MQ=255)
                                                                          cTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTGATGACTACACCAATGACCAGtctc                                            >  3:250162/1‑135 (MQ=255)
                                                                          cTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTctct                                           <  2:279816/136‑1 (MQ=255)
                                                                          cTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTctct                                           <  2:275311/136‑1 (MQ=255)
                                                                            tAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAg                                                                                                        <  4:259674/73‑1 (MQ=255)
                                                                            tAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAg                                                                                                        >  3:259674/1‑73 (MQ=255)
                                                                             aaGTGCTCCTTTGGTATAAGGTGTATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGTCGTTCGATTGTATGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTc                                         <  6:22322/135‑1 (MQ=255)
                                                                                      tttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTctct                                           <  3:204612/124‑1 (MQ=255)
                                                                                      tttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTctct                                           >  4:204612/1‑124 (MQ=255)
                                                                                      tttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑GTTTTTTTGGCTAAAATTGAAGGAGGCGGTCGAATGTACCCAACGCATGCCCGAGAAGTTCATGTATACACCAATGCCCAGTCTCTTCAATGTGAGCg                               >  6:46694/1‑136 (MQ=255)
                                                                                        tGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCg                               <  2:310468/134‑1 (MQ=255)
                                                                                        tGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCg                               <  5:179282/134‑1 (MQ=255)
                                                                                         ggTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCg                               <  4:148955/133‑1 (MQ=255)
                                                                                          gTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCt                           >  7:220835/1‑136 (MQ=255)
                                                                                           tAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTc                          <  5:343919/136‑1 (MQ=255)
                                                                                           tAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTc                          <  7:166947/136‑1 (MQ=255)
                                                                                             aaaGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAATTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGCCCAGTCGCTTCAATGTGACCGACCTCGc                        >  5:302005/1‑136 (MQ=255)
                                                                                             aaaGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGc                        <  8:166961/136‑1 (MQ=255)
                                                                                                  tGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGCTTGTACGCATCGCATGCCGGTGAAGTTAATGAGTTCACCAATGACCAGTCTCTTCAATTTGAGCGAGCTCGCCggc                    >  3:223428/1‑133 (MQ=255)
                                                                                                  tGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGaa                                                                        >  4:160622/1‑83 (MQ=255)
                                                                                                  tGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGaa                                                                        <  3:160622/83‑1 (MQ=255)
                                                                                                  tGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTc                    >  4:38132/1‑135 (MQ=255)
                                                                                                                 cATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGGTCATTACTACACCAATTACCAGTCTCTTCAATGTGAGCGACCGCGCCGTCATCTGAGCGGAACg      >  8:159304/1‑134 (MQ=255)
                                                                                                                   ttGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGGAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCCTGGCGACACCAATGACCAGTCGGTTACATGGGAGCGACCTCGCGGTCATCTGAGCGGCACGGAGc  >  2:188870/1‑136 (MQ=255)
                                                                                                                            |                                                                                                                               
ATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAACGAAGC  >  ACB143/44169‑44420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑