Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	1,309,639	C→G	P2A (CCC→GCC)	aaeB_2 →	p‑hydroxybenzoic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	1,309,639	0	C	G	100.0%	261.0 / NA	68	P2A (CCC→GCC)	aaeB_2	p‑hydroxybenzoic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (37/31);  total (37/31)

TCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCATTGA  >  ACB143/1309507‑1309771                                                                                                                                     |                                                                                                                                     tCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGgcc                                                                                                                                    <  1:79536/135‑1 (MQ=255)   tGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCa                                                                                                                                   >  4:138975/1‑134 (MQ=255)     aGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCAt                                                                                                                                  <  8:36468/133‑1 (MQ=255)      gCTGGCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCAcc                                                                                                                              <  7:284234/136‑1 (MQ=255)            ccTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAg                                                                                                                        <  6:22058/136‑1 (MQ=255)            ccTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAg                                                                                                                        <  1:280189/136‑1 (MQ=255)             cTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAgg                                                                                                                       >  1:106223/1‑136 (MQ=255)             cTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAgg                                                                                                                       >  1:18980/1‑136 (MQ=255)             cTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAgg                                                                                                                       <  8:232545/136‑1 (MQ=255)                  aGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTg                                                                                                                  >  1:143902/1‑136 (MQ=255)                  aGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTg                                                                                                                  >  1:300541/1‑136 (MQ=255)                   gTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTg                                                                                                                  <  4:339056/135‑1 (MQ=255)                     gCAGGCCAATCGAATATACATGATTAGCTAGATAATTAATCGTTTCCGCTTTATACTGCTCATCATTGTCTATCTTTTCAGAAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATAACCTTTCATGCACTGTTc                                                                                                               >  2:65221/1‑136 (MQ=255)                       aGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTcgc                                                                                                             >  1:383170/1‑136 (MQ=255)                       aGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTcg                                                                                                              >  3:144816/1‑135 (MQ=255)                       aGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTc                                                                                                               <  3:253034/134‑1 (MQ=255)                         gCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTcgcg                                                                                                            >  3:24103/1‑135 (MQ=255)                         gCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCgcgc                                                                                                           >  5:316792/1‑136 (MQ=255)                         gCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGAAAGGTCCTCCTCGTGGCCATCACCTTTCAGGCCCTGTTagcg                                                                                                            >  3:20216/1‑135 (MQ=255)                            aaTCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGcc                                                                                                          >  6:34310/1‑134 (MQ=255)                            aaTCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCca                                                                                                        >  8:270472/1‑136 (MQ=255)                                gAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCcagcag                                                                                                    <  1:147919/136‑1 (MQ=255)                                gAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCcagca                                                                                                     <  8:15248/135‑1 (MQ=255)                                 aaTATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCcagcag                                                                                                    >  4:107614/1‑135 (MQ=255)                                 aaTATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCagcagc                                                                                                   >  1:355980/1‑136 (MQ=255)                                 aaTATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCagcagc                                                                                                   >  6:74268/1‑136 (MQ=255)                                      aCATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCg                                                                                               >  3:174272/1‑135 (MQ=255)                                      aCATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGc                                                                                              >  2:22908/1‑136 (MQ=255)                                      aCATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGAGCCCAGCAGCCTcaa                                                                                              >  5:235322/1‑134 (MQ=255)                                            ttAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCt                                                                                           >  3:326225/1‑133 (MQ=255)                                            ttAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCa                                                                                         <  3:19901/135‑1 (MQ=255)                                              agctagctAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGt                                                                                      >  1:267092/1‑136 (MQ=255)                                              agctagctAAATAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAACACTTTTCCCCGCAATGTCCGCCTCGTGGCCATCACCTATCAGGCCATGTTCGCGCCCAGCAGCCTCGCCCTCAAGt                                                                                      >  5:282574/1‑136 (MQ=255)                                               gctagctaATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGtt                                                                                     >  7:320092/1‑136 (MQ=255)                                               gctagctaATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGt                                                                                      <  6:234223/135‑1 (MQ=255)                                                   gctaATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGcc                                                                                 <  6:75609/136‑1 (MQ=255)                                                     taattaatCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCa                                                                                <  4:165609/135‑1 (MQ=255)                                                          tctCGTTTCCGCTATTTCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGGCCGCCTCGTGGCCATCACCTTTGAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCATGTTTGCCATCAAg                                                                           <  8:266289/133‑1 (MQ=255)                                                          aatCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAg                                                                           <  8:297888/135‑1 (MQ=255)                                                                   cGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCg                                                                 <  1:22908/136‑1 (MQ=255)                                                                    gCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCgg                                                                >  7:80944/1‑136 (MQ=255)                                                                    gCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCgg                                                                >  5:327338/1‑136 (MQ=255)                                                                    gCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGACTCGCCCTCAATTTTGCCATCAAGACCCTGCTCgg                                                                >  7:103599/1‑136 (MQ=255)                                                                    gCTTTATCCTGCTCATCATTGTCTATCTTTTCAGAAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCg                                                                 >  3:308214/1‑135 (MQ=255)                                                                                  atcatTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGt                                                  >  8:287166/1‑136 (MQ=255)                                                                                  atcatTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGATAGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGt                                                  >  8:359499/1‑136 (MQ=255)                                                                                       tGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTATGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGtgg                                             >  5:133455/1‑136 (MQ=255)                                                                                           tATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGtggctg                                          <  3:59728/135‑1 (MQ=255)                                                                                            aTCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAg                                                                                       >  1:41025/1‑89 (MQ=255)                                                                                            aTCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAg                                                                                       <  2:41025/89‑1 (MQ=255)                                                                                            aTCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGtggctggc                                        >  1:47722/1‑136 (MQ=255)                                                                                             tCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGtggctggc                                        <  6:357525/135‑1 (MQ=255)                                                                                               ttttCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGa                                      >  3:305848/1‑135 (MQ=255)                                                                                                 ttCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATgc                                   >  2:232856/1‑136 (MQ=255)                                                                                                 ttCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCATGATCGGGGGCGGCCTGGCGTTGTGGCTGGCGATgc                                   >  8:26085/1‑136 (MQ=255)                                                                                                  tCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATgc                                   <  1:17371/135‑1 (MQ=255)                                                                                                  tCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATgc                                   <  5:353849/135‑1 (MQ=255)                                                                                                   cAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATgcg                                  <  6:316792/135‑1 (MQ=255)                                                                                                    aGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCAGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCt                                >  1:106680/1‑136 (MQ=255)                                                                                                      cAAGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTgg                              >  6:124089/1‑136 (MQ=255)                                                                                                       aaGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTggg                             <  2:5042/136‑1 (MQ=255)                                                                                                       aaGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTggg                             <  6:281225/136‑1 (MQ=255)                                                                                                       aaGACTTTTCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTGCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTggg                             <  1:23546/136‑1 (MQ=255)                                                                                                               tCCCCGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCGGGCGATGCGCTGGGGGCTg                        <  4:326225/133‑1 (MQ=255)                                                                                                                tcccGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAg                     <  8:287817/134‑1 (MQ=255)                                                                                                                 cccGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAg                     <  3:138975/134‑1 (MQ=255)                                                                                                                 cccGCAAGGTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCa                   <  1:32578/136‑1 (MQ=255)                                                                                                                        ggTCCGCCTCGTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACcctcc            <  1:364578/136‑1 (MQ=255)                                                                                                                                  gTGGCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCATtga  <  1:217517/136‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     TCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTCCGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCATTGA  >  ACB143/1309507‑1309771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑