Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 4,759,509 G→A intergenic (+124/‑16) fpvA_3 → / → ligM_KToptimized Ferripyoverdine receptor/sequence generated by OPTIMIZER for KT2440

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,759,5090GA100.0% 169.5 / NA 48intergenic (+124/‑16)fpvA_3/ligM_KToptimizedFerripyoverdine receptor/sequence generated by OPTIMIZER for KT2440
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/25);  total (23/25)

GCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTC  >  ACB143/4759378‑4759638
                                                                                                                                   |                                                                                                                                 
gcgtCTGATCGCCTGACGTGTAACGCAATAAACCGCAACCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                               <  6:112243/133‑1 (MQ=21)
gCTTCTGATCGCCTGACGTTTAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                               <  5:153790/136‑1 (MQ=255)
gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                               <  6:154203/136‑1 (MQ=255)
gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                               <  6:289968/136‑1 (MQ=255)
gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                               <  7:213977/136‑1 (MQ=255)
gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                               <  7:295067/136‑1 (MQ=255)
 cTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                               <  4:346554/135‑1 (MQ=255)
 cTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                               <  3:72478/135‑1 (MQ=255)
    cTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag                                                                                                                             >  2:109144/1‑134 (MQ=255)
    cTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag                                                                                                                             >  1:299224/1‑134 (MQ=255)
    cTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag                                                                                                                             >  1:263089/1‑134 (MQ=255)
    cTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTa                                                                                                                              >  4:91109/1‑133 (MQ=255)
           ccTGACGTTGAACGCAAAAAACCGCACCCAGGTGCTTTTTTTTGATCTGTTTACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCAGACCAACACATAGAGTTTAGGAGGTATAc                                                                                                                    <  6:272397/136‑1 (MQ=25)
              gACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACAt                                                                                                                  >  1:321797/1‑135 (MQ=255)
                 gTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAgc                                                                                                              <  8:169236/136‑1 (MQ=255)
                 gTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAgc                                                                                                              <  8:174398/136‑1 (MQ=255)
                 gTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAg                                                                                                               <  3:333676/135‑1 (MQ=255)
                      aCGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGccc                                                                                                          <  3:62015/135‑1 (MQ=255)
                      aCGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGccc                                                                                                          <  4:152694/135‑1 (MQ=255)
                        gCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGa                                                                                                       >  2:245326/1‑136 (MQ=255)
                           aaaaaCCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTa                                                                                                    <  8:99570/136‑1 (MQ=255)
                                           tGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag                                                                                                                             >  1:161954/1‑95 (MQ=255)
                                           tGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag                                                                                                                             <  2:161954/95‑1 (MQ=255)
                                             cGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATACTGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTgg                                                                                  <  2:308105/136‑1 (MQ=255)
                                               gTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGATTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGgcc                                                                                <  1:3174/136‑1 (MQ=255)
                                               gTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGgcc                                                                                <  5:186447/136‑1 (MQ=255)
                                                               aCAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACAcc                                                                 >  4:244992/1‑135 (MQ=255)
                                                                 aaTTATTGATCGGCTCGTATAATGTGTGGAATTGTGAGCTGATAACAATTTCACACCAACTCATAGAGTTTAGGAGGTATACATGAGGGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCg                                                                <  3:243097/134‑1 (MQ=37)
                                                                         atcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGtt                                                      >  5:29489/1‑136 (MQ=255)
                                                                          tcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTg                                                     <  8:133825/136‑1 (MQ=255)
                                                                                         tgtgGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGa                                        <  4:133596/134‑1 (MQ=255)
                                                                                           tgGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTgg                                    >  6:321705/1‑136 (MQ=255)
                                                                                            ggAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgc                                   <  6:113892/136‑1 (MQ=255)
                                                                                            ggAATTGTGAGCGGATAACAATATCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTgg                                    >  4:22340/1‑135 (MQ=255)
                                                                                              aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                 >  6:113284/1‑136 (MQ=255)
                                                                                              aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                 >  2:282996/1‑136 (MQ=255)
                                                                                              aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                 >  5:319948/1‑136 (MQ=255)
                                                                                              aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                 >  5:295499/1‑136 (MQ=255)
                                                                                              aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcg                                  >  4:296073/1‑135 (MQ=255)
                                                                                              aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcg                                  >  4:253199/1‑135 (MQ=255)
                                                                                              aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcg                                  >  4:313275/1‑135 (MQ=255)
                                                                                              aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgc                                   <  3:111190/134‑1 (MQ=255)
                                                                                              aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTACCAGATTGgctc                                 >  8:237562/1‑134 (MQ=255)
                                                                                                      gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTc                          >  5:157468/1‑135 (MQ=255)
                                                                                                          aTAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGTCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTAc                       <  8:271131/134‑1 (MQ=255)
                                                                                                           tAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTAccc                     >  4:72010/1‑135 (MQ=255)
                                                                                                                       acCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCgcgc          >  5:63934/1‑134 (MQ=255)
                                                                                                                              caTAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGCAGCAGGTGCTCGCCGCCGGCGGCCACCCCGTTGAAATGTTGCGTAACTCACAGATTGGCGCCTCCGACTACCCGGTCGTCCCGCCAGAGtag  >  4:181029/1‑133 (MQ=255)
                                                                                                                                   |                                                                                                                                 
GCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTC  >  ACB143/4759378‑4759638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑