Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB143 | 687,469 | C→A | 6.4% | P87Q (CCA→CAA) | CJ019_00644 → | Omega‑amino acid‑‑pyruvate aminotransferase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB143 | 687,469 | 0 | C | A | 6.4% | 207.6 / 4.1 | 63 | P87Q (CCA→CAA) | CJ019_00644 | Omega‑amino acid‑‑pyruvate aminotransferase |
Reads supporting (aligned to +/- strand): ref base C (30/29); new base A (2/2); total (32/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.85e-01 |
ACCACGGGCGCAAGATCTTCGACGCCCTGTCCGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTCCGAGTGCGCCGATACCGCACTGAAGATGGTG > ACB143/687352‑687602 | aCCACGGGCGCAAGATCTTCGACGCCCTGTCCGGCCTGTGGACCTGCGGCGCAGGGCACACTCTCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCa > 1:343642/1‑136 (MQ=255) ggCGCAAGATCTTCGACGCCCTGTCCGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCt > 1:10529/1‑136 (MQ=255) gcgcAAGATCTTCGACGCCCTGTCCGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCAAGGAAGTCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTg > 6:274919/1‑136 (MQ=255) gcgcAAGATCTTCGACGCCCTGTCCGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTg > 4:344238/1‑136 (MQ=255) gcgcAAGATCTTCGACGCCCTGTCCGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTg > 6:195405/1‑136 (MQ=255) cGCCCTGTCCGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCg > 6:305162/1‑136 (MQ=255) tGTCCGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGaaa > 3:310377/1‑135 (MQ=255) gTCCGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGaa > 2:213307/1‑133 (MQ=255) gTCCGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGaa > 2:239877/1‑133 (MQ=255) gTCCGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCAACCCGCTGTCGTTGCAGCTGGCGGAAAAg > 2:322921/1‑136 (MQ=255) tCCGGCCTGTGGACCTGCGGCGCAGGGCACACTCTCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGaaaa < 2:343642/134‑1 (MQ=255) ccGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCCAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGAt < 6:321204/136‑1 (MQ=255) ccGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGAt < 3:323352/136‑1 (MQ=255) cGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGAt < 8:286907/135‑1 (MQ=255) cGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGAt < 2:87050/135‑1 (MQ=255) cTGTGGANCTGCGGCGCAGGGCNCACNCGCAANGAAATNGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCg < 6:376059/136‑1 (MQ=255) gtgGACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAg > 5:126972/1‑136 (MQ=255) gACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTg < 2:30154/136‑1 (MQ=255) aCCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCCGAAAATATAGCCGAGCAgg > 2:182056/1‑136 (MQ=255) ccTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGt > 4:362846/1‑136 (MQ=255) ccTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGt > 4:11666/1‑136 (MQ=255) cggcgCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTcc < 6:141154/135‑1 (MQ=255) gcgcAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCggg < 3:241933/136‑1 (MQ=255) cgcAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCCGCAGGCGGATAAAATCGCCGACCGGGGTCCGGGc > 6:238741/1‑136 (MQ=255) cgcAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGc < 5:326772/136‑1 (MQ=255) cAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCa > 5:235952/1‑135 (MQ=255) cacTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAAt < 2:295122/136‑1 (MQ=255) cacTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAAt < 8:249247/136‑1 (MQ=255) cacTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAAt < 4:52960/136‑1 (MQ=255) acTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAAt > 8:244071/1‑135 (MQ=255) cGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACg > 7:233374/1‑136 (MQ=255) cGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATATGAATCaag > 8:89601/1‑134 (MQ=255) aaGTAAATCGCTGACGAGGTGACCCGTCAACTGAGTACGCTGGACTAATCCCCAGCGTTCCAGTTCGGCAACCCGGTGTCTTTCAAGCTGGCGTAAAAGATCGCCGAGCTGGTTCCGGGAAATCTGAATCACGt < 3:218035/134‑1 (MQ=255) ggAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTACAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGtcttc > 7:46296/1‑136 (MQ=255) aaTCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCtat < 5:195405/136‑1 (MQ=255) aaTCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCtat < 1:182056/136‑1 (MQ=255) aaTCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCtat < 6:126972/136‑1 (MQ=255) gCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGCGCGGGTTCCGGGCAATCTGAATCACGTCTTCTATAcc > 4:187782/1‑135 (MQ=255) gCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATAcc > 7:117450/1‑135 (MQ=255) gCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCAAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAAt > 1:385992/1‑120 (MQ=255) gCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCAAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAAt < 2:380482/120‑1 (MQ=255) gCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCAAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAAt < 2:385992/120‑1 (MQ=255) gCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCAAGCGTTCCAGTTCGGCCACCCGCTGTCGTTACAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAAt > 1:380482/1‑120 (MQ=255) cTGACGCTGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGGTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCaa < 8:36946/136‑1 (MQ=255) tGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGGAATCTGACTCCCGTCTTCTTTACCAAc > 7:204018/1‑136 (MQ=255) ggTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGt < 7:13767/136‑1 (MQ=255) aCCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGCAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTCCg > 2:268470/1‑136 (MQ=255) aCCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTcc < 8:47397/135‑1 (MQ=255) aCCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTcc < 1:112549/135‑1 (MQ=255) aCCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTcc < 1:93310/135‑1 (MQ=255) aCCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTcc < 2:272073/135‑1 (MQ=255) cccGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTCCGa < 2:39301/136‑1 (MQ=255) cTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTCCGAGTGCGcc > 3:215873/1‑135 (MQ=255) cTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTCCGAGTGCGCCg < 5:286926/136‑1 (MQ=255) aGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTCCGAGTGCGCCGa < 8:139881/134‑1 (MQ=255) aGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTCCGAGTGCGCCGATa < 6:313157/136‑1 (MQ=255) aCGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTCCGAGTGCGCCGATACCg > 4:402729/1‑136 (MQ=255) cGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCCACTCCGGTTCCGAGTGCGCCGCTACCg > 5:132776/1‑135 (MQ=255) gCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTCCGAGTGCGCCGATACCGCa > 7:96112/1‑136 (MQ=255) cTGGACTACTCCACAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCCAGCTGGGTCCGGGCGATGTGAATGACCGCGTCGATACCCACTGAGGTTCCGAGTGCGGCGATACCGCAc > 2:247058/1‑136 (MQ=255) ggACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCATTCTGAATCACGTCTTCTATACCAACTCCGGTTCCGAGTGCGCCGATACCGCACTg < 4:310377/136‑1 (MQ=255) gACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTCCGAGTGCGCCGATACCGCACTGa < 1:280025/136‑1 (MQ=255) actCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTCCGAGTGCGCCGATACCGCACTGAAGAt > 8:28121/1‑136 (MQ=255) cccAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTCCGAGTGCGCCGATACCGCACTGAAGATGgtg > 1:240652/1‑136 (MQ=255) | ACCACGGGCGCAAGATCTTCGACGCCCTGTCCGGCCTGTGGACCTGCGGCGCAGGGCACACTCGCAAGGAAATCGCTGACGCGGTGACCCGTCAACTGAGTACGCTGGACTACTCCCCAGCGTTCCAGTTCGGCCACCCGCTGTCGTTCCAGCTGGCGGAAAAGATCGCCGAGCTGGTTCCGGGCAATCTGAATCACGTCTTCTATACCAACTCCGGTTCCGAGTGCGCCGATACCGCACTGAAGATGGTG > ACB143/687352‑687602 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |