Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,176,644 C→A 7.2% G265V (GGC→GTC)  mltF_1 ← Membrane‑bound lytic murein transglycosylase F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,176,6440CA 7.2% 181.1 / 4.1 56G265V (GGC→GTC) mltF_1Membrane‑bound lytic murein transglycosylase F
Reads supporting (aligned to +/- strand):  ref base C (27/25);  new base A (2/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01

GCTGCCAGCAGGCGCCAGTCGACCTGCTCGACCTTGGCGTAGCTCTTGAAGTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCGGCCACTGCCCAGCGCTGGTCGCGGGTTTCGCCCAGA  >  ACB143/1176512‑1176778
                                                                                                                                    |                                                                                                                                      
gCTGCCAGCAGGCGCCAGTCGACCTGCTCGACCTTGGCGTAGCTCTTGAAGTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCg                                                                                                                                      >  5:205077/1‑135 (MQ=255)
       gcagGCGCCAGTCGACCTGCTCGACCTTGGCGTAGCTCTTGAAGTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCg                                                                                                                              >  2:190712/1‑136 (MQ=255)
                             gtccTTGGCGTAGCTCTTGAAGTGCTTCTGGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTg                                                                                                        <  2:175191/134‑1 (MQ=255)
                             gACCTTGGCGTAGCTCTTGAAGTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTg                                                                                                        <  1:142286/136‑1 (MQ=255)
                                    gCGTAGCTCTTGAAGTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGtttt                                                                                                 <  1:353300/136‑1 (MQ=255)
                                       tAGCTCTTGAAGTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTcg                                                                                              >  8:206433/1‑135 (MQ=255)
                                       tAGCTCTTGAAGTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCt                                                                                              >  8:373657/1‑136 (MQ=255)
                                       tAGCTCTTGAAGTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCt                                                                                              >  2:27823/1‑136 (MQ=255)
                                       tAGCTCTTGAAGTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCt                                                                                              >  7:19846/1‑136 (MQ=255)
                                         gCTCTTGAAGTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCtgt                                                                                            <  2:325262/136‑1 (MQ=255)
                                            ctTGAAGTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGcc                                                                                         >  8:37327/1‑136 (MQ=255)
                                                 aGTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCGTTGTc                                                                                    >  5:355822/1‑136 (MQ=255)
                                                 aGTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTGTTTCTGTGACTTGTc                                                                                    >  2:333983/1‑136 (MQ=255)
                                                  gTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGt                                                                                     >  7:311665/1‑134 (MQ=255)
                                                  gTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGt                                                                                     <  8:311665/134‑1 (MQ=255)
                                                        ctcGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATAc                                                                             >  5:146186/1‑136 (MQ=255)
                                                        ctcGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATAc                                                                             >  7:336227/1‑136 (MQ=255)
                                                        ctcGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTCTGCCTTGTCgagaaa                                                                              >  5:31130/1‑133 (MQ=255)
                                                                   gCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTGTTTCTGTGCCTTGTCGAGATACTCGTTGATTTc                                                                  >  7:267707/1‑136 (MQ=255)
                                                                       gcgcTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTg                                                              <  3:102863/136‑1 (MQ=255)
                                                                        cgcTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGa                                                             <  5:91043/136‑1 (MQ=255)
                                                                            gTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGCTTTCGTTGAGCAg                                                         >  8:295735/1‑136 (MQ=255)
                                                                             ttGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAgg                                                        <  5:123245/136‑1 (MQ=255)
                                                                             ttGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAgg                                                        <  2:129906/136‑1 (MQ=255)
                                                                             ttGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAgg                                                        <  1:190712/136‑1 (MQ=255)
                                                                             ttGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAgg                                                        <  6:282627/136‑1 (MQ=255)
                                                                             gtGCAGGTGCGGGGCGAAGGGGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCAGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAgg                                                        <  8:330230/135‑1 (MQ=255)
                                                                               gCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCt                                                      >  3:381421/1‑136 (MQ=255)
                                                                                    tGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCtgttgt                                                 >  1:47390/1‑136 (MQ=255)
                                                                                          gCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGc                                           <  3:186704/136‑1 (MQ=255)
                                                                                                gtgtAGGCGCCGACATAGCCATGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTCCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCgg                                     <  5:242093/136‑1 (MQ=255)
                                                                                                gtgtAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTATCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCgg                                     <  5:256524/136‑1 (MQ=255)
                                                                                                gtgtAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCgg                                     <  8:309934/136‑1 (MQ=255)
                                                                                                gtgtAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCgg                                     <  8:336227/136‑1 (MQ=255)
                                                                                                gtgtAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCgg                                     <  8:77823/136‑1 (MQ=255)
                                                                                                gtgtAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCgg                                     <  1:161521/136‑1 (MQ=255)
                                                                                                gtgtAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCgg                                     <  3:54873/136‑1 (MQ=255)
                                                                                                gtgtAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCgg                                     <  3:75181/136‑1 (MQ=255)
                                                                                                gtgtAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCgg                                     <  5:2780/136‑1 (MQ=255)
                                                                                                 tgtAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCGGc                                    >  4:321469/1‑136 (MQ=255)
                                                                                                  gtAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTAGTTGAGCAGGCTGTTGTCTTCGACTGCGGc                                    >  5:355885/1‑135 (MQ=255)
                                                                                                     ggCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCGGCCACt                                <  5:396808/136‑1 (MQ=255)
                                                                                                      gcgcCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCGGCCACTg                               >  4:393351/1‑136 (MQ=255)
                                                                                                        gcCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCGGCCACTGcc                             >  6:350903/1‑136 (MQ=255)
                                                                                                             cATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCGGCCACTGCCCAgcg                        >  3:391112/1‑136 (MQ=255)
                                                                                                             cATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCAGTGCCTTTTAGAGATACTCGTTGATTTCGTTTAGCAGGCTGATGTCTTAGCCTGCGGCCACTGCCCAgcg                        >  3:38812/1‑136 (MQ=255)
                                                                                                              aTAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCGGCCACTGCCCAgcgc                       >  4:17235/1‑136 (MQ=255)
                                                                                                                         cGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCATGCTGTTGTCTTCGCCTGCGGCCACTGCCCAGCGCTGGTCGCGGGt            >  8:328597/1‑136 (MQ=255)
                                                                                                                         cGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCGGCCACTGCCCAGCGCTGGTCGCGGGt            >  7:18172/1‑136 (MQ=255)
                                                                                                                             gACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCGGCCACTGCCCAGCGCTGGTCGCGGGTTTc         <  1:370083/135‑1 (MQ=255)
                                                                                                                             gACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCGGCCACTGCCCAGCGCTGGTCGCGGGTTTCg        <  2:234952/136‑1 (MQ=255)
                                                                                                                               cATGGCCGTATTAGCGGTCCTTCAATCGCTGAAGGCTGCCGAGTTTCAGTGCCGTGTACTTAAACGCGGTGATTTCGTAGCGCAGGCAGTTGTCCTCGCCTGCGGCCCCTGCCCATCGCTGGTCGCGGGTTTCTcc      >  4:254980/1‑136 (MQ=255)
                                                                                                                               cATGGACGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCg                                            >  7:194738/1‑98 (MQ=255)
                                                                                                                               cATGGACGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCg                                            >  7:189319/1‑98 (MQ=255)
                                                                                                                               cATGGACGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCg                                            <  8:189319/98‑1 (MQ=255)
                                                                                                                               cATGGACGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCg                                            <  8:194738/98‑1 (MQ=255)
                                                                                                                                    ccGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCGGCCACTGCCCAGCGCTGGTCGCGGGTTTCGCCCAGa  >  5:94602/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                                      
GCTGCCAGCAGGCGCCAGTCGACCTGCTCGACCTTGGCGTAGCTCTTGAAGTGCTTCTCGTACTTGGGCAGGCGCTGTTGCAGGTGCTGGGCGAAGGTGTAGGCGCCGACATAGCCAAGTACGTCGACATGGCCGTAGTAGCGGTCCTTCAATCGCTGAAGGGTGCCGTTTTTCTGTGCCTTGTCGAGATACTCGTTGATTTCGTTGAGCAGGCTGTTGTCTTCGCCTGCGGCCACTGCCCAGCGCTGGTCGCGGGTTTCGCCCAGA  >  ACB143/1176512‑1176778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑