Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,287,317 C→A 5.2% A37S (GCA→TCA)  CJ019_01209 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,287,3170CA 5.2% 270.2 / 2.4 76A37S (GCA→TCA) CJ019_01209hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (27/45);  new base A (2/2);  total (29/47)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

ATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGC  >  ACB143/1287182‑1287452
                                                                                                                                       |                                                                                                                                       
aTTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCAGCGCCAACCTTTCCAGCATTAACGCGCGATTTTCAGGATCGGCCCCAGc                                                                                                                                         >  5:110060/1‑136 (MQ=255)
     tCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCGTACGCCCCACCTTCTCCTGCCGCGCATGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGc                                                                                                                                     >  6:341804/1‑135 (MQ=255)
     tCAAGCTACACCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCcgcg                                                                                                                                    >  1:342998/1‑136 (MQ=255)
      cAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGa                                                                                                                                   >  2:68800/1‑136 (MQ=255)
       aaGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGa                                                                                                                                   <  3:113689/135‑1 (MQ=255)
          cTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAAc                                                                                                                               <  1:298408/136‑1 (MQ=255)
          cTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAAc                                                                                                                               <  8:208138/136‑1 (MQ=255)
           tACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGACCCTGCCAGCGACAACg                                                                                                                              >  5:5102/1‑136 (MQ=255)
           tACCCCGGATAACCTGAAGAATCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACg                                                                                                                              >  5:174800/1‑136 (MQ=255)
            aCCCCGGATAACCTGCCGCCTCGCGCTCCACCCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCCGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGc                                                                                                                             >  6:190219/1‑136 (MQ=255)
                cGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAgg                                                                                                                         >  3:74019/1‑136 (MQ=255)
                 ggATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGCCAACGCCCGGt                                                                                                                        >  5:60668/1‑136 (MQ=255)
                 ggATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGt                                                                                                                        >  3:274007/1‑136 (MQ=255)
                  gATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTc                                                                                                                       <  1:26089/136‑1 (MQ=255)
                      aCCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACACCGCCAGGTCGCgg                                                                                                                   >  8:345095/1‑136 (MQ=255)
                          gccgccTAGCGCTCAAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTATACAGAATGAACGCGCGTTTATCAGTATAGGCCCCTGCAAGCCACAACTCCAGGTCGCgggcga                                                                                                               >  4:212714/1‑132 (MQ=255)
                                   cgcTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAgcggc                                                                                                      <  2:354226/136‑1 (MQ=255)
                                    gcTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAgcggcg                                                                                                     >  6:60726/1‑136 (MQ=255)
                                     cTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGgcgc                                                                                                    <  7:81340/136‑1 (MQ=255)
                                        cAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCAt                                                                                                  <  6:348046/135‑1 (MQ=255)
                                        cAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCAt                                                                                                  <  2:155004/135‑1 (MQ=255)
                                        cAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCAt                                                                                                  <  1:268002/135‑1 (MQ=255)
                                        cAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCAt                                                                                                  <  3:74165/135‑1 (MQ=255)
                                        cAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATg                                                                                                 <  6:268876/136‑1 (MQ=255)
                                                  gCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATAc                                                                                        >  4:45892/1‑135 (MQ=255)
                                                     aGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAacc                                                                                    <  6:106353/136‑1 (MQ=255)
                                                     aGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAacc                                                                                    <  7:55772/136‑1 (MQ=255)
                                                     aGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAacc                                                                                    <  4:163716/136‑1 (MQ=255)
                                                     aGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAacc                                                                                    <  7:89114/136‑1 (MQ=255)
                                                     aGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAacc                                                                                    <  2:247741/136‑1 (MQ=255)
                                                          cTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAg                                                                               <  1:257053/136‑1 (MQ=255)
                                                                  ccACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATa                                                                       <  7:268379/136‑1 (MQ=255)
                                                                  ccACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATa                                                                       <  3:345479/136‑1 (MQ=255)
                                                                  ccACCTTATCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCATGATCGGCCCCAGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATa                                                                       <  6:3880/136‑1 (MQ=255)
                                                                       ttCTCCTGCCGCGCCTGCGCCAACCTATCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCCTCCCGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTc                                                                  >  5:183001/1‑136 (MQ=255)
                                                                         ctcCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTc                                                                  <  5:163363/134‑1 (MQ=255)
                                                                         ctcCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTc                                                                  <  3:169725/134‑1 (MQ=255)
                                                                            cTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTccc                                                                <  2:342998/133‑1 (MQ=255)
                                                                            cTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCg                                                               <  6:320164/134‑1 (MQ=255)
                                                                            cTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCg                                                               <  5:30377/134‑1 (MQ=255)
                                                                            cTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGACAGCGACAACGCCAGGTCGCGGTCCATCCAgcggc                                                                                                      >  6:50604/1‑95 (MQ=255)
                                                                            cTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGACAGCGACAACGCCAGGTCGCGGTCCATCCAgcggc                                                                                                      <  5:50604/95‑1 (MQ=255)
                                                                            cTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGACAGCGACAACGCCAGGTCGCGGTCCATCCAgcggc                                                                                                      <  5:46053/95‑1 (MQ=255)
                                                                            cTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGACAGCGACAACGCCAGGTCGCGGTCCATCCAgcggc                                                                                                      >  6:46053/1‑95 (MQ=255)
                                                                            cTGCCGCGCCTGCGCCAACCTATCCAGCATGTACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCg                                                               <  7:107129/134‑1 (MQ=255)
                                                                               ccGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACg                                                          >  7:131985/1‑136 (MQ=255)
                                                                                  cgcCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACggtg                                                       >  6:59789/1‑136 (MQ=255)
                                                                                     cTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACggtggt                                                     >  2:184634/1‑135 (MQ=255)
                                                                                        cgcCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCGTGCCTGCGAAAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGTAAATACAGTCCCGCAACGGTGGTGAc                                                  <  6:110060/135‑1 (MQ=255)
                                                                                           cAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCacg                                              >  3:180067/1‑136 (MQ=255)
                                                                                            aaCCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCacga                                             <  3:180239/136‑1 (MQ=255)
                                                                                            aaCCTCTCAAGCATTAACGCGAGTTTTTCTGGCTCTGCCCCTTCCAGCGACATCGCCAGGTCGCGTTCCATCCAGCGTATCATGCGCACAACAAACCCCCAGTGGAAAAACAGTCCCGCAACGGTGGTTACAacga                                             <  8:366515/136‑1 (MQ=255)
                                                                                                  tCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATg                                        <  1:15018/135‑1 (MQ=255)
                                                                                                   ccAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGa                                       <  4:274007/135‑1 (MQ=255)
                                                                                                     aGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAg                                                                               >  6:178712/1‑93 (MQ=255)
                                                                                                     aGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAg                                                                               <  5:236573/93‑1 (MQ=255)
                                                                                                     aGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAg                                                                               >  6:236573/1‑93 (MQ=255)
                                                                                                     aGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAg                                                                               <  5:178712/93‑1 (MQ=255)
                                                                                                     aGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCACCGGTGGTGACCACGACGATGAAg                                     >  5:27779/1‑135 (MQ=255)
                                                                                                     aGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAg                                     >  5:263495/1‑135 (MQ=255)
                                                                                                       cATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTaa                                  <  4:321964/136‑1 (MQ=255)
                                                                                                       cATGAACGCGCGTTTATCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTaa                                  >  7:48446/1‑136 (MQ=255)
                                                                                                         tGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATc                                <  7:131552/136‑1 (MQ=255)
                                                                                                            aCGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATg                                        >  7:219352/1‑125 (MQ=255)
                                                                                                            aCGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATg                                        <  8:219352/125‑1 (MQ=255)
                                                                                                            aCGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCAt                             <  7:65405/136‑1 (MQ=255)
                                                                                                            aCGCGCGTTTTTCAGGATCGCCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCAt                             <  5:210401/136‑1 (MQ=255)
                                                                                                               cgcgTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGc                          >  4:33835/1‑136 (MQ=255)
                                                                                                               cgcgTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGc                          >  6:218106/1‑136 (MQ=255)
                                                                                                                  gTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTAt                       <  7:383874/136‑1 (MQ=255)
                                                                                                                       tCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCtt                   <  6:169186/135‑1 (MQ=255)
                                                                                                                                gCCCCTGCCAGCGACAACGCCCGGTCGCGGTCAATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACtt         <  8:289573/136‑1 (MQ=255)
                                                                                                                                  cccTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGa       <  1:244008/136‑1 (MQ=255)
                                                                                                                                  cccTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGa       <  1:377612/136‑1 (MQ=255)
                                                                                                                                  cccTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGa       <  3:394736/136‑1 (MQ=255)
                                                                                                                                  cccTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAAGCCATGGCTATTCTTGGTCTCACTTGa       <  3:332772/136‑1 (MQ=255)
                                                                                                                                   ccTGCCATCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGa       >  1:287341/1‑135 (MQ=255)
                                                                                                                                       ccAGCGACAACGCCAGGTCGCGGTCCATCCAGGGGCGAATGCGCACATACAACCACCAGTGTAAATACAGTCCCGCAACGGTGGTTACAACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGc  <  3:47097/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
ATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCCCCTGCCAGCGACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGC  >  ACB143/1287182‑1287452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑