Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	2,332,171	C→T	5.9%	A308A (GCC→GCT)	ybhH →	Putative isomerase YbhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	2,332,171	0	C	T	5.9%	226.3 / 2.8	67	A308A (GCC→GCT)	ybhH	Putative isomerase YbhH
Reads supporting (aligned to +/- strand):  ref base C (29/34);  new base T (2/2);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGTTCGCTATTTCATGCCGCACAACTGCCATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGTCTACGCCCCT  >  ACB143/2332037‑2332303                                                                                                                                       |                                                                                                                                     cAGGTTCGCTATTTCATGCCGCACAACTGCCATCGTGCCTTGGAGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGccc                                                                                                                                     <  3:291362/136‑1 (MQ=255)  aGGTTCGCTATTTCATGCCGCACAACTGCCATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCa                                                                                                                                    <  3:64470/136‑1 (MQ=255)        gCTATTTCATGCCGCACAACTGCCATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTcc                                                                                                                               <  7:185450/135‑1 (MQ=255)         cTATTTCATGCCGCACAACTGCCATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCCCCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCTGCAACTGGCCCAGGTCCGc                                                                                                                             >  6:205742/1‑136 (MQ=255)         cTATTTCATGCCGCACAACTGCCATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGc                                                                                                                             <  2:82192/136‑1 (MQ=255)          tATTTCATGCCGCACAACTGCCATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGc                                                                                                                             >  5:217977/1‑135 (MQ=255)            tttCATGCCGCACAACTGCCATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTg                                                                                                                          >  4:85447/1‑136 (MQ=255)            tttCATGCCGCACAACTGCCATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTg                                                                                                                          <  5:270354/136‑1 (MQ=255)            tttCATGCCGCACAACTGCCATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTg                                                                                                                          <  6:124427/136‑1 (MQ=255)            tttCATGCCGCACAACTGCCATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCGGGCGACTGCCTGCGTCAGCGCTGGCACCGTGATTGCCGAGCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCt                                                                                                                           <  4:25108/135‑1 (MQ=255)                   ccGCACAACTGCCATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCACCTGGAGCAcc                                                                                                                   <  1:16506/136‑1 (MQ=255)                     gCACAACTGCCATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCAccc                                                                                                                  >  7:377889/1‑135 (MQ=255)                              ccATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTggcgg                                                                                                         <  2:182326/135‑1 (MQ=255)                              ccATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTggcgg                                                                                                         <  6:217977/135‑1 (MQ=255)                                   gTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCg                                                                                                    <  1:317639/135‑1 (MQ=255)                                      ccTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATg                                                                                                 <  7:250511/135‑1 (MQ=255)                                        ttGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTg                                                                                              >  1:187275/1‑136 (MQ=255)                                        ttGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTg                                                                                              >  5:289710/1‑136 (MQ=255)                                           gCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCCGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTAgcgc                                                                                           >  8:24546/1‑136 (MQ=255)                                             gATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCt                                                                                          >  3:285297/1‑135 (MQ=255)                                             gATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTg                                                                                         <  5:382699/136‑1 (MQ=255)                                                        cGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCTCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCgg                                                                              >  2:120110/1‑136 (MQ=255)                                                         ggTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGt                                                                             <  3:128015/136‑1 (MQ=255)                                                         ggTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGt                                                                             <  3:69130/136‑1 (MQ=255)                                                           tGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGc                                                                           >  8:233543/1‑136 (MQ=255)                                                            ggggCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGcc                                                                          >  1:271778/1‑136 (MQ=255)                                                             gggCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGTCCCAGGTCCGCCTGGAGCACCCAAGGGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCg                                                                         >  8:262370/1‑136 (MQ=255)                                                              ggCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGcc                                                                          >  2:177774/1‑134 (MQ=255)                                                                   gCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGc                                                                    >  2:406917/1‑135 (MQ=255)                                                                          cctgcGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACg                                                              <  1:316129/134‑1 (MQ=255)                                                                          cctgcGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGAt                                                            <  1:353351/136‑1 (MQ=255)                                                                            tgcGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATcc                                                          >  2:183232/1‑136 (MQ=255)                                                                            tgcGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATc                                                           >  1:199413/1‑135 (MQ=255)                                                                             gcGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATc                                                           >  1:252398/1‑134 (MQ=255)                                                                               gTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAgg                                                       <  5:181971/136‑1 (MQ=255)                                                                                       tGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCTCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCggtg                                               >  3:384151/1‑136 (MQ=255)                                                                                         gCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCggtggt                                             <  3:75643/136‑1 (MQ=255)                                                                                           aCCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCTCATTCCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGc                                           <  3:226318/136‑1 (MQ=255)                                                                                           aCCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGc                                           >  3:395620/1‑136 (MQ=255)                                                                                                 aTTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTggcgg                                                                                                         <  4:131435/68‑1 (MQ=255)                                                                                                 aTTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTggcgg                                                                                                         >  3:131435/1‑68 (MQ=255)                                                                                                 aTTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCg                                     >  7:63260/1‑136 (MQ=255)                                                                                                 aTTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCg                                     >  6:200988/1‑136 (MQ=255)                                                                                                  ttGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGCTCCAGGCTTCGGTGGTGCGAACCGc                                    >  8:331086/1‑136 (MQ=255)                                                                                                  ttGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGc                                    >  4:169377/1‑136 (MQ=255)                                                                                                  ttGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGCCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGc                                    >  3:330765/1‑136 (MQ=255)                                                                                                        aCCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCcggcg                              <  1:183232/136‑1 (MQ=255)                                                                                                        aCCTGTTGGGCGAAGGCGCGCAACAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCcggcg                              <  4:405902/136‑1 (MQ=255)                                                                                                             ttGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGctct                         <  4:321785/136‑1 (MQ=255)                                                                                                              tGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCtctt                        >  8:366383/1‑136 (MQ=255)                                                                                                               gggCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCtcttc                       >  6:75948/1‑136 (MQ=255)                                                                                                                ggCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCtcttct                      >  1:238686/1‑136 (MQ=255)                                                                                                                   gAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAg                   <  7:177487/136‑1 (MQ=255)                                                                                                                   gAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAg                   <  1:406262/136‑1 (MQ=255)                                                                                                                        cgcgcAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTc              <  7:354567/136‑1 (MQ=255)                                                                                                                          cgcAGCAACTGGCTCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGt            <  3:26401/136‑1 (MQ=255)                                                                                                                          cgcAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGt            <  6:69088/136‑1 (MQ=255)                                                                                                                          cgcAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGt            >  6:74714/1‑136 (MQ=255)                                                                                                                          cgcAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGt            >  3:62104/1‑136 (MQ=255)                                                                                                                          cgcAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGt            <  5:21028/136‑1 (MQ=255)                                                                                                                          cgcAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGt            <  2:395306/136‑1 (MQ=255)                                                                                                                          cgcAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGGGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGt            <  4:59260/136‑1 (MQ=255)                                                                                                                            cagcaACTGGCCCAGGTCCGCCTGGCGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGTCt          <  4:100503/136‑1 (MQ=255)                                                                                                                            cagcaACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGTCt          <  6:122840/136‑1 (MQ=255)                                                                                                                             agcaACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGATGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGTGGCTCTTCTCAGGGTTCGTCTa         >  1:100816/1‑136 (MQ=255)                                                                                                                               caACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGTCTACg       <  4:182420/136‑1 (MQ=255)                                                                                                                                  cTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGTCTACGccc    <  6:120680/136‑1 (MQ=255)                                                                                                                                     tcccAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGTCTACGCCCCt  <  5:10661/134‑1 (MQ=255)                                                                                                                                     gCCCAGGTCCGCCTGGAGCACCCAAGTGCCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGTCTACGCCCCt  <  3:72888/135‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     CAGGTTCGCTATTTCATGCCGCACAACTGCCATCGTGCCTTGGCGATCACTGGAGCGGTGGGGCTGGCGACTGCCTGCGTCAGCCCTGGCACCGTGATTGCCGACCTGTTGGGCGAAGGCGCGCAGCAACTGGCCCAGGTCCGCCTGGAGCACCCAAGTGGCGGCATCGATGTTGCGCTGACGCGCAGCGGTGCCGAGGGCCAGACGATCCAGGCTTCGGTGGTGCGAACCGCCCGGCGGCTCTTCTCAGGGTTCGTCTACGCCCCT  >  ACB143/2332037‑2332303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑