Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,703,424 T→C 12.0% R321R (CGT→CGC)  CJ019_02554 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,703,4240TC12.0% 133.4 / 8.3 51R321R (CGT→CGC) CJ019_02554hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (23/21);  new base C (2/4);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTGAATGACCCTGCTTGAGGGAAG  >  ACB143/2703290‑2703558
                                                                                                                                      |                                                                                                                                       
gggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGtt                                                                                                                                        >  2:388635/1‑136 (MQ=255)
gggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGAAGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGtt                                                                                                                                        >  2:254971/1‑136 (MQ=255)
  gCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTgg                                                                                                                                      >  1:48618/1‑136 (MQ=255)
    aTTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCt                                                                                                                                    >  3:348190/1‑136 (MQ=255)
           ccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGTCCAAGGCCAGGCGTTGGCTGAAGCTg                                                                                                                             >  8:133035/1‑136 (MQ=255)
                gCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTAGTGCAGGAAATCGCTCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTgg                                                                                                                         >  1:82831/1‑135 (MQ=255)
                 cGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATACCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGaa                                                                                                                       <  5:22331/136‑1 (MQ=255)
                               agatgatGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGAAGGCGAGCGCGAACGCCGTATACCGGTATAGCAGGAAATAGATCAGCACAAGGCCAGGCTATGGCTGAAGCTCTTGGAAAGGGACGACTACgc                                                                                                         >  4:291041/2‑135 (MQ=255)
                                      gcACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGAt                                                                                                  >  4:83738/1‑136 (MQ=255)
                                       cccGAGGAGGCCCACGGGCGGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCGGAAGCGGTTGGAAAGGGACGACTACGGCGTCGAt                                                                                                  <  4:407018/133‑1 (MQ=255)
                                       cACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGGGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGAt                                                                                                  <  3:285958/135‑1 (MQ=255)
                                          gatgatGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTc                                                                                              >  7:184095/1‑136 (MQ=255)
                                              atgCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCg                                                                                          <  2:250967/136‑1 (MQ=255)
                                               tgCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGt                                                                                         >  3:237330/1‑136 (MQ=255)
                                                gCCCACGGGCGGGCGTGGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGtt                                                                                        <  3:99774/136‑1 (MQ=255)
                                                 cccACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGtt                                                                                        <  6:219750/135‑1 (MQ=255)
                                                    aCGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAg                                                                                    >  2:34980/1‑136 (MQ=255)
                                                    aCGGGCTGGCGTTGAACAGCGACGGAGTGAGCGAACGCCGTATTCCGGTATTGAAGGAAATCGATCAGGCCAAGGCCAGGCGCTGGCTGAATCTGTTGGAAAGGGACGACTACGCCCTCGATTTACGCCTTTACAg                                                                                    >  7:286813/1‑136 (MQ=255)
                                                         cTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGcc                                                                                >  4:335850/1‑135 (MQ=255)
                                                               ttGAACCGCGACGGCGGGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGCTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggc                                                                         <  5:291863/136‑1 (MQ=255)
                                                                tGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGtt                                                                                        >  2:86472/1‑120 (MQ=255)
                                                                tGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGtt                                                                                        <  1:86472/120‑1 (MQ=255)
                                                                tGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggcg                                                                        >  4:204163/1‑136 (MQ=255)
                                                                     cgcgACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGgccgc                                                                   >  6:394497/1‑136 (MQ=255)
                                                                     cgcgACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGgccgc                                                                   >  1:38735/1‑136 (MQ=255)
                                                                       cgACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGccc                                                                 <  8:184095/136‑1 (MQ=255)
                                                                          cGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCggg                                                              >  1:370869/1‑136 (MQ=255)
                                                                          cGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCggg                                                              >  4:356153/1‑136 (MQ=255)
                                                                          cGGCGTGCGCGAACGCCGAATACCGGTATTGCAGGAAATCGATCACGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTTCCGAAAGGCGGCCGCCCggg                                                              >  5:395422/1‑136 (MQ=255)
                                                                                  gcgAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGGTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGGTACAGTGCCCACGGGCGGCCGCCCGGGTACCGTTa                                                      >  4:88475/1‑136 (MQ=255)
                                                                                               tCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGAGTTTCGGCGTTACAGTGCCGAAGGGCGGCCTCCCGGGTACCGTTACAAGTTTCTTGCg                                         >  5:34513/1‑136 (MQ=255)
                                                                                                      ttGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCgg                                  <  7:330091/136‑1 (MQ=255)
                                                                                                       tGCAGGAAATCGATCAGGCCAAGGCCAGGCGCTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGt                                 >  8:95612/1‑136 (MQ=255)
                                                                                                         cAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACACTGCCGAAGGGCGGCCGCACCGGTACAGTTACACGTTTATGGCGTCAcccggc                                >  3:253883/1‑131 (MQ=255)
                                                                                                          aGGAAATCGATCAGGCCAAGGCCAGGCGCTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGa                              <  1:221376/136‑1 (MQ=255)
                                                                                                            gAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTTCCGTTTCCCGTTTCTGGCGGGACCGGGCGAat                            >  6:33808/1‑136 (MQ=255)
                                                                                                              aaTCGATCAGGCCAAGGCCAGGCGCTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAat‑at                         <  4:330046/136‑1 (MQ=255)
                                                                                                                tCGCTCAGGCCAAGGCCAGGCGTTCGCTGAAGCTGTTGGAAAGGGCCGACTACCGCGTCGATTTTCGGCGTTACAGTGCAGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTg                       <  5:20011/136‑1 (MQ=255)
                                                                                                                tCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTg                       <  8:97072/136‑1 (MQ=255)
                                                                                                                tCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTg                       <  8:379963/136‑1 (MQ=255)
                                                                                                                    tCAGGCGACGGCCAGGCGTGGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCTATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTGAATg                   <  8:133971/136‑1 (MQ=255)
                                                                                                                    tCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTGAATg                   <  7:60937/136‑1 (MQ=255)
                                                                                                                    tCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGGCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTGAATg                   <  7:273396/136‑1 (MQ=255)
                                                                                                                    tCAGGCCAAGGCCAGGCGTTGGCTGAAGCGGTTGGAAAGGGACGACTAGGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTGAATg                   <  2:137634/136‑1 (MQ=255)
                                                                                                                    tCAGGCCAAGGCCAGGCGCTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTGAATg                   <  3:183570/136‑1 (MQ=255)
                                                                                                                         ccaaggccaGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTGAATGACCCt              <  7:276669/136‑1 (MQ=255)
                                                                                                                         ccaaggccaGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTGAATGACCCt              <  2:330565/136‑1 (MQ=255)
                                                                                                                           aaggccaGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTAGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGATTCTGGCGTCACCGGTCGAAT‑ATTGAATTAcacatc            >  8:121117/1‑131 (MQ=255)
                                                                                                                               ccaGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTGAATGACCCTGCTTGa        <  2:370869/136‑1 (MQ=255)
                                                                                                                                caGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTGAATGACCCTGCTTGAg       <  7:248865/136‑1 (MQ=255)
                                                                                                                                 aGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTGAATGACCCTGCTTGAgg      <  1:6342/136‑1 (MQ=255)
                                                                                                                                     gTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTGAATGACCCTGCTTGAGGGAAg  >  1:91261/1‑136 (MQ=255)
                                                                                                                                     gTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTACGCCTTACACTGCCGAATGGCGGCCCCCCGGGTACCCTTACCCGTTTCTGGCGGGACCGGTCGAATAATTTAAT‑CCCCTGCTTGTgggtat  >  6:35289/1‑133 (MQ=255)
                                                                                                                                      |                                                                                                                                       
GGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTCACCGGTCGAAT‑ATTGAATGACCCTGCTTGAGGGAAG  >  ACB143/2703290‑2703558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑