Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 3,267,793 T→G 23.0% L171V (TTG→GTG)  eamA → putative amino‑acid metabolite efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,267,7930TG23.0% 75.1 / 9.0 53L171V (TTG→GTG) eamAputative amino‑acid metabolite efflux pump
Reads supporting (aligned to +/- strand):  ref base T (14/26);  new base G (11/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 3.13e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTGGCACTGATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCCTGGC  >  ACB143/3267659‑3267917
                                                                                                                                      |                                                                                                                            
gccTGGCACTGATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTGCGGCTCGGTCGACCTGGTCGCGtt                                                                                                                             <  4:41851/136‑1 (MQ=255)
         tGATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTg                                                                                                                    <  5:325499/136‑1 (MQ=255)
           aTCGGCAGCGAAGACGGCGGCAATGTGCCCTTGCTTGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGTCCTGGGCAACATCATTACCCGACGTTTCGGCTCGGTCGACCTGTTCGCGTTGGTGATCTg                                                                                                                    <  6:393895/134‑1 (MQ=255)
              ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGt                                                                                                                <  6:293044/135‑1 (MQ=255)
              ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTg                                                                                                               >  4:138409/1‑136 (MQ=255)
              ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATCTGGGGTg                                                                                                               >  7:315940/1‑136 (MQ=255)
              ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGGGATCTGGGGTg                                                                                                               >  4:207070/1‑136 (MQ=255)
                   cGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGTTGGGGGGGGGGGTg                                                                                                          >  4:210724/1‑136 (MQ=255)
                        aCGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATGTGGGGTGGGGTGATaac                                                                                                     >  2:330379/1‑134 (MQ=255)
                        aCGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGAGGTTGGGTGGGGTGATAcc                                                                                                     >  2:146178/1‑136 (MQ=255)
                             ggcCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGTTGTGGGGGGGGGGGGTACCGcccc                                                                                                >  6:243869/1‑134 (MQ=255)
                              gcCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACt                                                                                               <  4:352558/136‑1 (MQ=255)
                              gcCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACt                                                                                               <  4:380302/136‑1 (MQ=255)
                              gcCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGGGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACt                                                                                               <  7:190761/136‑1 (MQ=255)
                                        cATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                     <  3:251377/136‑1 (MQ=255)
                                        cATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                     <  3:359327/136‑1 (MQ=255)
                                        cATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                     <  6:326492/136‑1 (MQ=255)
                                        cATGCTGGCGTTGGTGCTGACCCTGGGTGGCGGGGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                     <  1:379431/136‑1 (MQ=255)
                                         aTGCTGGGGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTgg                                                                                    <  3:47339/136‑1 (MQ=255)
                                         aTGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCTACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTgg                                                                                    <  7:203165/136‑1 (MQ=255)
                                            cTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGGGCTCTGGGGGGGGGTGATACCGCCCCTTACATTCCTGGccc                                                                                 >  7:217067/1‑134 (MQ=255)
                                                           aCCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCt                                                                    <  3:138409/134‑1 (MQ=255)
                                                              cTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGGGATCAGGGGTGGGCGGTTACCGCCCCATCCCATACTGGCCCGGTCCAGGTGGCTGGCAg                                                               >  4:92353/1‑136 (MQ=255)
                                                                 tgtgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGcc                                                            <  6:262710/136‑1 (MQ=255)
                                                                 ggtgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGcc                                                            <  6:268506/135‑1 (MQ=255)
                                                                   tgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGcccc                                                          >  2:79805/1‑136 (MQ=255)
                                                                   tgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGccc                                                           <  3:160384/135‑1 (MQ=255)
                                                                       ggTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAgc                                                      <  8:315940/136‑1 (MQ=255)
                                                                        gTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAgcg                                                     >  5:161207/1‑136 (MQ=255)
                                                                        gTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAgcg                                                     >  8:14231/1‑136 (MQ=255)
                                                                            gATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGTTCTGGGGTGGGGTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCAtt                                                 >  5:53493/1‑136 (MQ=255)
                                                                             aTGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTg                                                <  5:262746/136‑1 (MQ=255)
                                                                             aTGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATCTGGGGTGGGCTGATACCGCCCCTTCCATTACTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCAttt                                                >  5:132907/1‑135 (MQ=255)
                                                                              tgtgGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGTTCTGGGGTGGGGTGGTACCGCCCCTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGCGCGCATTgg                                               >  4:151318/1‑136 (MQ=255)
                                                                              tgtgGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACt                                                                                               <  7:95423/88‑1 (MQ=255)
                                                                              tgtgGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACt                                                                                               >  8:95423/1‑88 (MQ=255)
                                                                                  gggCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAt                                           <  8:4162/136‑1 (MQ=255)
                                                                                  gggCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAt                                           <  6:300581/136‑1 (MQ=255)
                                                                                  gggCCTGGGCAACATCATAACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGGGGCTGGAAGGCCCCGAGCGCATTGGCCAt                                           <  5:61119/136‑1 (MQ=255)
                                                                                      cTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGAACTGGTCGAGTTGGTGATATGTGGTGGGCTGAAAACCCCACATCAATTACTGGCAAATTCCTGGTGGCAGGAACGCCCCGCCCGCATTGACCaatcca                                       >  8:271180/1‑131 (MQ=255)
                                                                                         ggaaaCATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTgg                                    <  5:333362/133‑1 (MQ=255)
                                                                                           cAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGc                                   >  2:321109/1‑135 (MQ=255)
                                                                                            aaCATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTTGTTATCTGGGGTTGGCTGATAACGCCACTTCAATTCCTGTCACGTTACTGGTGGCTGGGAGGCCACGAGCGCATTGGCAATGAATTGGcc                                  >  4:67188/1‑135 (MQ=255)
                                                                                                    tACACGACGGTTCGGCTCGGTCGACCTGGACGCGTTGGTGAACTGCGGTGGTCTGATACCCCCCCTTCCATTCCTGGAACTGTCCAGGTGGCGTGAAGGACCCGAGCGCATTTGCCATGCCTTGGCCAAAATCAGt                         >  8:241314/1‑136 (MQ=255)
                                                                                                      cccGACGGTTCGGCTCGGTCGCCCTGGTCGCGGTTGTGATCTGGGGGGGGGGGGTACCGCCCCTTCCCTTACTGGCCCGGTCCTGGTGGCGGGTAGGGCCCGCGCGCCATGGCCCGGCCATGGCCACTATCAGTTg                       >  2:188589/1‑136 (MQ=255)
                                                                                                      cccGACGGTTCGGCTCGGTCGACCTGGTCGCGGTTGTGATCTGGGGTGGGGTGCTACCGCCACTTGCCATGCTGGCACGGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGGCCTGGCTTGGGCCATTTCAGTTg                       >  5:263771/1‑136 (MQ=255)
                                                                                                            ggTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACATCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGt                   >  8:224084/1‑134 (MQ=255)
                                                                                                             gTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAATTCg                <  7:108021/136‑1 (MQ=255)
                                                                                                              ttCGGCGCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCgg               <  5:173372/136‑1 (MQ=255)
                                                                                                                 ggCTCGGTCGACCTGGTCGCGGTGGGGTTCTGGGGTGGGCGGGTACCGCCCCGTACATTACTGGCCCTGTCCTGGTGGCGGGCAGGCCCCGAGCGCAATGGCCATTCCGTGGCCAAAATAAGTTGGAGTTCGGTGc            >  2:211225/1‑136 (MQ=255)
                                                                                                                   cTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTg          <  2:23790/136‑1 (MQ=255)
                                                                                                                       gTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGccc      <  7:145215/136‑1 (MQ=255)
                                                                                                                       gTCGACCTGGTCGCGTTGGTGAGCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGccc      <  7:268217/136‑1 (MQ=255)
                                                                                                                           aCCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGTCATGCCTTGGCCAATATAAGTTGGTGTTGGGTGGTGGCCCTggg  >  1:404705/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                            
GCCTGGCACTGATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCCTGGC  >  ACB143/3267659‑3267917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑