| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 3,784,646 | T→G | 35.2% | D571A (GAC→GCC) | dmdC_4 ← | 3‑methylmercaptopropionyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 3,784,646 | 0 | T | G | 35.2% | 4.0 / 2.1 | 22 | D571A (GAC→GCC) | dmdC_4 | 3‑methylmercaptopropionyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (7/7); new base G (4/4); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 | |||||||||||
TCGGCGGACCGCTGCATGCGCTACCGCCCCTCGGCACGCCAGCCGCAGCCGGTGTTCATGACGACCTCGCACGTGGGTTTCAGAACGATTCGGCAATGAATGGCAACGGCGTGGCGGCCGCGT‑CGATGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCC > ACB143/3784524‑3784750 | tCGGCGGACCGCTGCATGCGCTACCGCCCCTCGGCACGCCAGCCGCAGCCGGTGTTCATGACGACCTCGCACGTGGGTTTCAGAACGATTCGGCAATGAATGGCAACGGCGTGGCGGCCGCGG‑CGATGgccgcccc > 7:83114/1‑134 (MQ=255)tCGGCGGACCGCGGCATGCGCACCCGCCCCTCGGCAGGCCAGCCGACGCCGGTGGTCATGCCGCCCTCGCAGGGGGGTTGCAGACCGATCCGGCAATGAATGGCACCGGCGTGGCGGCCGCGT‑CGATGGCCGCCAc < 4:235942/136‑1 (MQ=255) aCCGCTGCATGCGCTACCGCCCCTCGGCACGCCAGCCGCAGCCGGTGTTCATGACGACCTCGCACGTGGGTTTCAGAACGATTCGGCAATGAATGGCAACGGCGTGGCGGCCGCGG‑GGGTGGGCGCCCCCAGcccg > 7:160381/1‑134 (MQ=255) gCTGCATGCGCTACCGCCCCTCGGCACGCCAGCCGCAGCCGGTGTTCATGACGACCTCGCACGTGGGTTTCAGAACGATTCGGCAATGAATGGCAACGGCGTGGCGGCCGCGG‑CGATGGCCGCCCCCGGCCGGGCg > 8:304923/1‑136 (MQ=255) gcTACCGCCCCTCGGCACGCCAGCCGCAGCCGGTGTTCATGACGACCTCGCACGTGGGTTTCAGAACGATGCGGCAATGAATGGCAACGGCGTGGCGGCCCCGT‑GGATGGCCCCCCCCCGCCCGGCGCgggcgggc > 1:121762/1‑136 (MQ=255) gCCCCTCGGCACGCCAGCCGCAGCCGGTGTTCATGACGACCTCGCACGTGGGTTTCAGAACGATTCGGCAATGAATGGCAACGGCGTGGCGGCCGCGT‑CGATGGCCGCCCCCGGCCGGTCGAGGTCGGGCCGCAgg > 6:167765/1‑136 (MQ=255) ggCACGCCAGCCGCAGCCGGTGTTCATGACGACCTCGCACGTGGGTTTCAGAACGATTCGGCAATGAATGGCAACGGCGTGGCGGCCGCGG‑GGGTGGCCGCCACCTGCCGGTCGAGGGCGGGCCGCAGGtttccgg > 2:264888/1‑130 (MQ=255) gCACGCCAGCCGCAGCCGGTGTTCATGACGACCTCGCACGTGGGTTTCAGAACGTTTCGGAAATGAATGGCAACGGCGTGGCGGCCGCGT‑CTATGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAg < 7:382270/136‑1 (MQ=255) gCACGCCAGCCGCAGCCGGTGTTCATGACGACCTCGCACGTGGGTTTCAGAACGATTCGGCATTGAATGGCAACGGCGGGGCGGCCGCGG‑CGATGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAg < 7:304687/136‑1 (MQ=255) cACGCCAGCCGCAGCCGGTGTTCATGACGACCTCGCACGTGGGTTTCAGAACGATTCGGCAATGAATGGCAACGGCGTGGCGGCCCCGTGGGATGGCCCCCA‑CAGCCGGTCCATGTGGGGCACCAGGTATCCgcc > 7:372926/1‑133 (MQ=255) agccgcagccgGTGTTCATGACGACCTCGCACGTGGGTTTCAGAACGATTCGGCAATGAATGGCAACGGCGTGGCGGCCGCGT‑GGATGGCCGCCCCCAGCCCGGCGAGGGGGGGCCGCCGGGGGCCGAGGAAGAAc > 4:81914/1‑136 (MQ=255) gttattGACGACATTGCACGTGGGTTTCATACCTATTCGGCATTTAATGGAAACGGGGTGGGGGCGGCGT‑CGATGGCCGACACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCt < 8:322510/131‑1 (MQ=255) ttCATTACGCCCTCGCACGTGGGTTTCAGAACTATTCGCCAATGAATGGCACGGGGGTGGCGGCCGCGT‑CGATGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCt < 1:323877/135‑1 (MQ=255) cTCGCACGTGGGTTTCAGAACGATTCGGCAATGAATGGCAACGGCGTGGCGGGCCCGT‑CCGAGGCCCCCCCCAGCCCGGCGAGGTCGGGGCCCAGGGTGCCCCGGCAGCAACCAACGCGTTGCAGCTTTTaccc > 1:377885/1‑133 (MQ=255) cGCACGTGGGTTTCAGAACGATTCGGCAATGAATGGCAACGGCGTGGCGGCCGCGT‑CGATGGCCGCCCCCAGCCGGTCGAGGTCGGGCCGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCGGCTTTTACCCgcgc > 2:127189/1‑136 (MQ=255) tttCATAACGATTCGGAAATTAAGGGCAAGGGGGGGGGGGCGGCGT‑CGATGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGccc < 2:280585/136‑1 (MQ=255) taaCTATGCGGAAATGATGGGCACGGGGGGGGGGGCCGGGT‑CGAGGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACt < 2:330186/135‑1 (MQ=255) caaCTATTCGGCAATCAATGGCACCGGCGGGGCGGCCGCTG‑CGATGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACt < 7:22864/135‑1 (MQ=255) actaTTCGGCAATGAATGGCAACGGCGGGGCGGCCGCGT‑CGATGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTgg < 1:48253/133‑1 (MQ=255) aCGATTGGGCAATTATTGGCACCGGCGGGGGGGCGGGGG‑CGATGGCCCCCACCTGCCGGTCGCGGTCGGGCTGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTTCTTTTCCCCGCGCTGCGCCGCCCCCACt < 6:78307/134‑1 (MQ=255) aCGATTCGGCAATGAATGGCAACGGCGGGGCGGCCGCGG‑CGTGGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTgg < 7:19142/136‑1 (MQ=255) ggCAATGAATGGCAACGGCGTGGCGGCCGCGT‑CGATGGCCGCCACCAGCCGGGCGAGGTGGGGCACCACGTAGCCCAGGAAGAACCCCACGCTTTACTGCGTTTAACCGCGCATCCCCCCCCCCACAGGCAAcccc > 6:132581/1‑136 (MQ=255) | TCGGCGGACCGCTGCATGCGCTACCGCCCCTCGGCACGCCAGCCGCAGCCGGTGTTCATGACGACCTCGCACGTGGGTTTCAGAACGATTCGGCAATGAATGGCAACGGCGTGGCGGCCGCGT‑CGATGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCC > ACB143/3784524‑3784750 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |