Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 3,861,411 A→G 6.7% T17A (ACC→GCC)  gntT_2 → High‑affinity gluconate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,861,4110AG 6.7% 189.5 / 2.6 59T17A (ACC→GCC) gntT_2High‑affinity gluconate transporter
Reads supporting (aligned to +/- strand):  ref base A (23/32);  new base G (2/2);  total (25/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGACAGCGCTACCCCAACCCGTAAACGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCCAAGGTCATGAAATCGTTCCAG  >  ACB143/3861276‑3861539
                                                                                                                                       |                                                                                                                                
aaaGACAGCGCTACCCCAACCCGTAAACGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTa                                                                                                                                  >  4:330475/1‑136 (MQ=255)
    aCAGCGCTACCCCAACCCGTAAACGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCAt                                                                                                                              <  1:269112/136‑1 (MQ=255)
        cgcTACCCCAACCCGTAAACGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCAtcgtc                                                                                                                          <  4:200840/136‑1 (MQ=255)
          cTACCCCAACCCGTAAACGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACCATAAGACCGAGCCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCgg                                                                                                                        >  2:160021/1‑136 (MQ=255)
           tACCCCAACCCGTAAACGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCggg                                                                                                                       >  6:215592/1‑136 (MQ=255)
             ccccAACCCGTAAACGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGc                                                                                                                      <  6:318862/135‑1 (MQ=255)
             cccaAACCCGTAAACGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACAATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGc                                                                                                                      <  2:298552/135‑1 (MQ=255)
              cccAACCCGTAAACGCGGCAAAGACTCCAAAAAGCTACGCCCAAACCAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTg                                                                                                                    <  4:264795/136‑1 (MQ=255)
                      gTAAACGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCctgct                                                                                                            <  1:206814/136‑1 (MQ=255)
                         aaCGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATACGACCGAGGCCTTCACACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGAt                                                                                                         <  4:220090/136‑1 (MQ=255)
                          aCGCGGCTAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCTAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATc                                                                                                        <  3:71763/136‑1 (MQ=255)
                          aCGCGGCAAAGACTCCAACAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATc                                                                                                        <  8:353955/136‑1 (MQ=255)
                          aCGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGAt                                                                                                         >  8:130805/1‑135 (MQ=255)
                          aCGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATc                                                                                                        <  5:51865/136‑1 (MQ=255)
                          aCGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATc                                                                                                        <  3:11592/136‑1 (MQ=255)
                          aCGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATc                                                                                                        <  5:110374/136‑1 (MQ=255)
                          aCGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATc                                                                                                        <  5:348480/136‑1 (MQ=255)
                          aCGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATc                                                                                                        <  3:76611/136‑1 (MQ=255)
                          aCGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATc                                                                                                        <  1:354133/136‑1 (MQ=255)
                                 aaaGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACt                                                                                                 <  7:125617/136‑1 (MQ=255)
                                  aaGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACtt                                                                                                >  2:282194/1‑136 (MQ=255)
                                      cTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAg                                                                                            <  1:282194/136‑1 (MQ=255)
                                           aaaaGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCa                                                                                       >  6:383168/1‑136 (MQ=255)
                                             aaGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCAcc                                                                                     >  1:35628/1‑136 (MQ=255)
                                                  aCGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTtc                                                                                >  4:405408/1‑136 (MQ=255)
                                                       cAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGc                                                                           <  3:330475/136‑1 (MQ=255)
                                                                      aCCGAGGCCTTCAAACCATGTTCGGACTGTCTCCTGATCCCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTTATCACCCACTTCATGGTTCACCCCTTCGTCGCCCTCACCCTCGcggc                                                            <  4:330391/136‑1 (MQ=255)
                                                                          aGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGcggccggc                                                        <  1:112740/136‑1 (MQ=255)
                                                                           gcccTTCAAACCATGTTCGGACTGGCTACTTATACGTTACTGGTGCTGGACGCGCTTGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCCCCCGTTCGTCGCCCGCACCCTCGCGGCCGGCt                                                       <  2:387590/134‑1 (MQ=255)
                                                                             ccTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTc                                                     >  5:242919/1‑136 (MQ=255)
                                                                             ccTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTc                                                     >  6:346190/1‑136 (MQ=255)
                                                                             ccTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACACCTTCGTCGCCCTCACCCTCGCGGCCGGCTTc                                                     >  2:334711/1‑136 (MQ=255)
                                                                               ttCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCATCCt                                                   >  7:134544/1‑136 (MQ=255)
                                                                                 cAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCCCCCTCGCGGCCGGCTTCCTgg                                                 <  1:170069/136‑1 (MQ=255)
                                                                                 cAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTg                                                  <  2:275356/135‑1 (MQ=255)
                                                                                  aaaCCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTggg                                                <  4:47498/136‑1 (MQ=255)
                                                                                  aaaCCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTggg                                                >  4:150263/1‑136 (MQ=255)
                                                                                   aaCCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTGCCATCGTCGGGCTg                                                                                                                    >  1:264638/1‑67 (MQ=255)
                                                                                   aaCCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTGCCATCGTCGGGCTg                                                                                                                    >  1:270092/1‑67 (MQ=255)
                                                                                   aaCCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTGCCATCGTCGGGCTg                                                                                                                    <  2:264638/67‑1 (MQ=255)
                                                                                   aaCCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTGCCATCGTCGGGCTg                                                                                                                    <  2:270092/67‑1 (MQ=255)
                                                                                   aaCCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGc                                               >  3:360303/1‑136 (MQ=255)
                                                                                          ttCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCCCCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACtt                                        >  7:346850/1‑136 (MQ=255)
                                                                                                   gcaaCTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTTATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATg                                <  7:356786/132‑1 (MQ=255)
                                                                                                      aCTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCgg                            >  2:148352/1‑136 (MQ=255)
                                                                                                          aTACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTg                          <  4:192023/134‑1 (MQ=255)
                                                                                                          aTACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTg                          <  8:240451/134‑1 (MQ=255)
                                                                                                           tACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGcc                       <  8:113594/136‑1 (MQ=255)
                                                                                                           tACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGcc                       <  5:130331/136‑1 (MQ=255)
                                                                                                                tCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCCAAGGt                  >  5:133553/1‑136 (MQ=255)
                                                                                                                tCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCCAAGGt                  >  4:229660/1‑136 (MQ=255)
                                                                                                                tCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCAATTCAAGGTTCACCCCTTCGTCGCACTCACCCTCTCGGCCGGCATCCTGGGCCTGACTTCCGGCATGCCTGTGGCCAAGGt                  >  8:82554/1‑136 (MQ=255)
                                                                                                                 ccTGCTGCTCGACGCGCTGGTTACCATCGTCGGTCGGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCCAAGGt                  <  8:114009/135‑1 (MQ=255)
                                                                                                                    gctgctCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGATGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCCAAGGTCAt               >  5:387700/1‑135 (MQ=255)
                                                                                                                    gctgctCGACGCGCTGGTTACCATCGTCGGGCTCATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCCAAGGTCAt               >  3:209799/1‑135 (MQ=255)
                                                                                                                     ctgctCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCCAAGGTCATg              >  6:340690/1‑135 (MQ=255)
                                                                                                                           gACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCCAAGGTCATGAAATCGt       >  3:158323/1‑136 (MQ=255)
                                                                                                                           gACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCCAAGGTCATGAAATCGt       >  7:283700/1‑136 (MQ=255)
                                                                                                                                gcTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCCAAGGTCATGAAATCGTTCCAg  <  2:35628/136‑1 (MQ=255)
                                                                                                                                gcTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCCAAGGTCATGAAATCGTTCCAg  <  1:256552/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                
AAAGACAGCGCTACCCCAACCCGTAAACGCGGCAAAGACTCCAAAAAGCTACGCCCAAAACAACGATAAGACCGAGGCCTTCAAACCATGTTCGGACTGGCTACTGATACCTTCCTGCTGCTCGACGCGCTGGTTACCATCGTCGGGCTGATCCTGCTGATCACCCACTTCAAGGTTCACCCCTTCGTCGCCCTCACCCTCGCGGCCGGCTTCCTGGGCCTGACTTCCGGCATGCCGGTGGCCAAGGTCATGAAATCGTTCCAG  >  ACB143/3861276‑3861539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑