Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	193,160	T→A	16.2%	N2673K (AAT→AAA)	CJ019_00185 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	193,160	0	T	A	16.2%	159.8 / 2.8	74	N2673K (AAT→AAA)	CJ019_00185	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/40);  new base A (12/2);  total (32/42)
Fisher's exact test for biased strand distribution p-value = 5.75e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTT  >  ACB143/193025‑193294                                                                                                                                        |                                                                                                                                       aGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAAt                                                                                                                                        <  3:40018/136‑1 (MQ=38)   gCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGc                                                                                                                                      <  7:130994/136‑1 (MQ=40)   gCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCAGAGCAACAAAGc                                                                                                                                      >  3:140469/1‑136 (MQ=255)      aCGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGTCCCAGAGCAACAAAGCta                                                                                                                                    >  4:406282/1‑134 (MQ=255)      aCGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCa                                                                                                                                   <  4:402034/136‑1 (MQ=40)      aCGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCa                                                                                                                                   <  5:6859/136‑1 (MQ=40)      aCGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCa                                                                                                                                   <  5:379986/136‑1 (MQ=40)       cGACGGCGACATCGTCAAGGTCACCCTCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAg                                                                                                                                  <  7:262246/136‑1 (MQ=255)       cGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAg                                                                                                                                  <  4:391681/136‑1 (MQ=40)        gAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAgg                                                                                                                                 <  6:273233/136‑1 (MQ=40)          aGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCAGAGCAACAAAGCTGAggg                                                                                                                                >  7:350782/1‑134 (MQ=255)           ggCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGCCCCGGAGCAACAATCCTCAGGTcc                                                                                                                              >  8:236447/1‑135 (MQ=255)           ggCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCa                                                                                                                              >  2:4976/1‑136 (MQ=40)           ggCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAAAGCTCAGGTCa                                                                                                                              >  6:157255/1‑136 (MQ=37)           ggCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCAGAGCAACAATGCTAAGGTCa                                                                                                                              >  2:48749/1‑136 (MQ=255)               aCATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCa                                                                                                                           >  6:177946/1‑135 (MQ=39)                 aTCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCa                                                                                                                        <  1:127815/136‑1 (MQ=37)                    gTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAgg                                                                                                                     >  8:104580/1‑136 (MQ=37)                                aTCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGCCCCTGAGCAAAAAAGCGAAGGTCACCCTCAAGGCTGGCGAAACCa                                                                                                         >  1:54807/1‑136 (MQ=11)                                aTCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCa                                                                                                         <  5:361169/136‑1 (MQ=37)                                  cACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAgc                                                                                                       <  6:227302/136‑1 (MQ=37)                                  cACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCAGAGCAACAAAGCTCAGGTCACCATCAAGGCTGGCGAAACCAgc                                                                                                       >  6:314743/1‑136 (MQ=21)                                   acggaagACCAGACTTCGGTAGCCGAGAACGTCAACCCGACCTTCACCGTGCACGTAAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGATCAACAATGCTCAGGTCACCATCTAGGCTGGCGAAACCAgc                                                                                                       <  7:68111/129‑1 (MQ=11)                                   aCGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAgcg                                                                                                      <  7:217577/136‑1 (MQ=37)                                   aCGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAgc                                                                                                       <  7:46142/135‑1 (MQ=37)                                   aCGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAgc                                                                                                       <  5:342402/135‑1 (MQ=37)                                       ccGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCAGCGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCCGCGCGcc                                                                                                  >  1:257100/1‑136 (MQ=255)                                          aCCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTa                                                                                               <  2:61477/136‑1 (MQ=34)                                          aCCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTa                                                                                               <  3:310204/136‑1 (MQ=34)                                             aGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTGCCCATCAAGGCTGGCGAAACCAGCGCGCCGTaccc                                                                                            >  5:332333/1‑134 (MQ=255)                                               aCTTGGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGGTGGCCCACGAGCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACAccc                                                                                          <  6:97984/136‑1 (MQ=16)                                                    ggTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGAt                                                                                     <  1:40370/136‑1 (MQ=33)                                                     gTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATg                                                                                    <  2:409677/136‑1 (MQ=33)                                                      cggCCGAGAACGTCAACCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACACAGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGAGCCGTACACCCACGATgc                                                                                   <  5:112987/135‑1 (MQ=255)                                                       ggCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCCCCATCAAGGCTGGCGAAACCCGCGCGCCGTACACCCACGATgcg                                                                                  >  7:229128/1‑136 (MQ=21)                                                            agaACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAgg                                                                             <  5:246693/136‑1 (MQ=34)                                                            agaACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAgg                                                                             <  3:180196/136‑1 (MQ=34)                                                            agaACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCAGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGCAACCAGCGCGCCGTACCCCCCCGATGCGCaacg                                                                             >  4:369551/1‑134 (MQ=11)                                                            agaACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCAGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCACCCCGCCGGACACCCACCATGCGCAAgg                                                                             >  4:134377/1‑136 (MQ=11)                                                             gaACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGc                                                                            <  3:406767/136‑1 (MQ=34)                                                                cGTCAACCCGACCTTCAGGGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGAGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGAt                                                                         <  4:156835/136‑1 (MQ=255)                                                                cGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGAt                                                                         <  4:187342/136‑1 (MQ=34)                                                                        cGACCTTCACCGTGCAAGTCAACCATCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTa                                                                 <  4:373519/136‑1 (MQ=21)                                                                         gACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAAATCTCCGGTCCCCATCAACGCTGGCGCAACCAGCGCGCCGGACACCCCCGATGCGCCAGGCGATGACGGCTa                                                                 >  3:230647/1‑135 (MQ=11)                                                                             ttCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCAGACCAACAAAGCTCAGGTCACCATACAGGATGGCGAAAACACCGCGCCCAACACCCACCATTCGCAAAGCGATGACGTCTTACAgg                                                            >  6:209907/1‑136 (MQ=11)                                                                              tCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGCCCCTGAGCAAAAATGGTAACTGCACCATCAACGCTGGCGCAACCCGCGCGCCCTACCCCCCCGAGGCGCCAGGCGCTGTCCTGTATAAggc                                                           >  2:323047/1‑135 (MQ=2)                                                                              tCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTGAGGTCACCATAAAGGCTGGCGCAACCAGCGCGCCGGACACCCACGAAGCGCAAGGCGATGCCGTCTATCAGGa                                                           >  3:67830/1‑136 (MQ=11)                                                                              tCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCAGAGCAACAAATCTCAGGTCACCCTCAAAGCAGGCGAAACCCGCCCGCCGGACCCCCCCGATGCGCAAGGCGATGCCGGCTATCAGGa                                                           >  6:72171/1‑136 (MQ=11)                                                                               cACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAgg                                                            <  5:177946/134‑1 (MQ=34)                                                                                 ccGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGc                                                        <  1:65350/136‑1 (MQ=37)                                                                                  cGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCt                                                       <  5:378423/136‑1 (MQ=38)                                                                                       aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGGCCCTGAGCCAAAAAGCTCACGTAACCCACCAAGCTGGCGCAACCCACGCGCCCGGCCCCCACCAAGCGCAAGGCGATGACGGCTATCAGGGCGCTGGccc                                                  >  4:117578/1‑135 (MQ=2)                                                                                       aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGGCCCTGAGCAACAATGCTCATGTCACCAAAAAGGCTGGCGAAAACAGCGCGCCGTAAACACAAGAAGCGAAAGGACATGCCCTCTAACAGGCCGCTGGCCa                                                  >  8:118213/1‑136 (MQ=11)                                                                                       aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCCTCAAGGCTGGCGCAACCACCGCGCCGTACACCCACGATTCGCAAGGCGATGACGTCTATGAGGACCCTGGc                                                    >  8:120599/1‑134 (MQ=11)                                                                                       aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCa                                                  <  6:223695/136‑1 (MQ=40)                                                                                       aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCa                                                  <  5:352061/136‑1 (MQ=40)                                                                                       aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCa                                                  <  6:100567/136‑1 (MQ=40)                                                                                       aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGGACACCCACGAGGCGCAAGGCGATGACGTCTATCAGGACGCTGGc                                                    >  1:310224/1‑134 (MQ=255)                                                                                       aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCAGAGCAACCAAGCTCACGTGCCCCTCAACGCTGGCGAAACCCGCGCCCCGTACACCCCCGATGCGCAAGGCGATGACGGCGATCAGGACCCTGGc                                                    >  2:127070/1‑134 (MQ=11)                                                                                         gTCAACCAGCCGCTGGCCCACGACCTGGTCGTGCCCCTGAGCAACAATGCTCAGGTCCCCATCAAGGCTGGCGAAACCCGCGCGCCGTACACCCCCGATGCGCAAGGCGATGACGTCTATCAGGCCGCTGGCCAg                                                 >  2:106235/1‑135 (MQ=14)                                                                                         gTCAACCAGCCGCTGGCCCACGACCTGGTCGTGCCCCTGAGCAACAAAGCTCAGGTGACCAACAAGGCTGGCGAAACCAGCGCGCCGTACACCCACCAAGCGCAAGGCGATGACCTCTATAAGGACCCTGGc                                                    >  4:336205/1‑132 (MQ=11)                                                                                               cAGCCGCTGGCCCACGACCTGGTCGTGCCCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGcc                                          >  7:45802/1‑136 (MQ=255)                                                                                                   cGCTGGCCCACTACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTggg                                      <  7:144242/136‑1 (MQ=255)                                                                                                   cGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTggg                                      <  1:53934/136‑1 (MQ=255)                                                                                                        gCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCa                                 <  2:197297/136‑1 (MQ=255)                                                                                                        gCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCa                                 <  3:406282/136‑1 (MQ=255)                                                                                                        gCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCa                                 <  8:81179/136‑1 (MQ=255)                                                                                                                ccTGGTCGTGACCCTGAGCAACAAAGCTCAGGTCACCATCAAGGCTGGAGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCa                         <  7:139350/136‑1 (MQ=255)                                                                                                                    gTCGTGACCCTGAGCAACAATGCTCAGGTAACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATAAACTCGGCAGt                       <  1:4976/134‑1 (MQ=255)                                                                                                                             cTGAGCAACAATTCTCAGGTCACCCTCAAGGCTGGCGGAACCAGCGCGGCGTTCCCCCACCATGCGCAAGGCGATGACGTCTATCAGGGCGGGGGCCAGATCAGCCTGGGCATCAACTCGGCAGTTGACGccccgg            >  8:234905/1‑132 (MQ=14)                                                                                                                             cTGAGCAACAATTCGCAGGTCACCATAAAGGCGGGCGAAAACCGCGCGCCGGTCACCCACCAAGCGCAACGCGCTGACGGAGATCAGGCCGCGGGCCAGATCAGCCAGGGCATAAACTCGGCAGTAGACGCCAcag            >  2:105778/1‑134 (MQ=255)                                                                                                                                   aacaaTGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCt                                                       >  6:374332/1‑87 (MQ=255)                                                                                                                                   aacaaTGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCt                                                       <  5:374332/87‑1 (MQ=255)                                                                                                                                       aTGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGtt  <  2:286463/136‑1 (MQ=255)                                                                                                                                       aTGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGtt  <  8:370759/136‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       AGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTT  >  ACB143/193025‑193294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑