Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,037,737 A→G 6.3% I88V (ATT→GTT)  rhtB_3 → Homoserine/homoserine lactone efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,037,7370AG 6.3% 194.3 / 2.5 66I88V (ATT→GTT) rhtB_3Homoserine/homoserine lactone efflux protein
Reads supporting (aligned to +/- strand):  ref base A (28/32);  new base G (2/2);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGGGCGGGGTCTTGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCAAGGTGCTGCTGTTCTAT  >  ACB143/4037604‑4037865
                                                                                                                                     |                                                                                                                                
cGTGGGCGGGGTCTTGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCAtt                                                                                                                                >  6:263496/1‑136 (MQ=255)
   gggCGGGGTCTTGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCAGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCaa                                                                                                                             >  1:404041/1‑136 (MQ=255)
    ggCGGGGTCTTGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCaa                                                                                                                             <  5:205123/135‑1 (MQ=255)
     gCGGGGTCTTGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACt                                                                                                                           >  6:57545/1‑136 (MQ=255)
     gCGGGGTCTTGGGTTTCAGCGCCCTCATCGCCGTGACCATGTTCGGCCTCAGCGCACTGCTCCAACCCTCGGCGGCAGTGCTGAGCGTCCTGATGTGGGTGGGTGGGGCTTATGTGATCTGGCTGGGCATTGAACt                                                                                                                           >  7:87426/1‑136 (MQ=255)
        gggTCTTGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTGAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATg                                                                                                                        <  1:60778/136‑1 (MQ=255)
        gggTCTTGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATg                                                                                                                        <  8:81754/136‑1 (MQ=255)
        gggTCTTGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATg                                                                                                                        <  3:282360/136‑1 (MQ=255)
        gggTCTTGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATg                                                                                                                        <  2:40443/136‑1 (MQ=255)
        gggTCTTGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATg                                                                                                                        <  2:235398/136‑1 (MQ=255)
        gggTCTTGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGCCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATg                                                                                                                        <  1:81503/136‑1 (MQ=255)
        gggTCTTGGGGTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATg                                                                                                                        <  1:160695/136‑1 (MQ=255)
        gggTCTGGGGTTTCCGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATg                                                                                                                        <  7:319454/136‑1 (MQ=255)
          gTCTTGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGgc                                                                                                                      >  7:182766/1‑136 (MQ=255)
            cTTGGGTTTCAGCGCCCTCATAGCCTTGACCATTTTCTGCCTCAGAGCACTGCTACAAACCTCGGCTGCAGTGAAGAGCGACCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTTTGgcg                                                                                                                     >  7:196812/1‑135 (MQ=255)
             ttGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCa                                                                                                                   >  1:22745/1‑136 (MQ=255)
             ttGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCa                                                                                                                   >  1:16513/1‑136 (MQ=255)
             ttGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCa                                                                                                                   <  3:278327/136‑1 (MQ=255)
               gggTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAg                                                                                                                  <  2:69285/135‑1 (MQ=255)
                     cAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAgg                                                                                                            <  2:10449/135‑1 (MQ=255)
                      aGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGc                                                                                                           <  7:386742/135‑1 (MQ=255)
                        cgcCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGTCTGGGCATTCAACTATGGCGCAGTCCAGGccgg                                                                                                        >  3:11335/1‑134 (MQ=255)
                        cgcCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTg                                                                                                        >  4:337666/1‑136 (MQ=255)
                               aTCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTgg                                                                                                 <  2:16513/136‑1 (MQ=255)
                               aTCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCGGAGCGGCCTGAAGTGGGGGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTgg                                                                                                 <  2:22745/136‑1 (MQ=255)
                                 cGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGaa                                                                                               >  8:227762/1‑136 (MQ=255)
                                         ccATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGGTGCAGTGCGTAGCGTCAGTAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTAAACTATGGCGCAGTCCAGGCCTGCAGCTGTAACTGACcgc                                                                                       <  4:290506/136‑1 (MQ=255)
                                         ccATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACcgc                                                                                       <  2:270922/136‑1 (MQ=255)
                                          cATGTTCGGCCTCATCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACcgcg                                                                                      >  5:288247/1‑136 (MQ=255)
                                                cGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAgc                                                                                >  3:323450/1‑136 (MQ=255)
                                                          gcACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATg                                                                                                                        <  3:51045/86‑1 (MQ=255)
                                                          gcACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATg                                                                                                                        >  4:51045/1‑86 (MQ=255)
                                                          gcACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCCGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTgg                                                                      >  7:148755/1‑136 (MQ=255)
                                                          gcACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTgg                                                                      >  5:81833/1‑136 (MQ=255)
                                                                  ccAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGcc                                                              >  8:307568/1‑136 (MQ=255)
                                                                   cAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCg                                                             >  2:85293/1‑136 (MQ=255)
                                                                      cccacggcggCAGGGGTGAGCGGCCTGAAGTGGGTGGGGGGGGCTTATATGATCTGGCTGGGCATTCAACTAGGGCGCAGGCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGc                                                          <  8:315956/132‑1 (MQ=255)
                                                                      aCCTCGGCGGCAGTGCGGAGCGTCCTGACGTGGGGGGGTGGGGCTTATTTGATCGGGCTGGGCATTCAACTAAGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGc                                                          <  2:125516/136‑1 (MQ=255)
                                                                             cggcAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCg                                                   >  2:376940/1‑136 (MQ=255)
                                                                             cggcAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCCGCTGGGCAATGCCGGGCTGTATCg                                                   >  6:279239/1‑136 (MQ=255)
                                                                                        aGCGTCCTGAAGGGGGTGGGGGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTg                                         <  6:159342/135‑1 (MQ=255)
                                                                                          cGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCtt                                      >  1:220465/1‑136 (MQ=255)
                                                                                                   gtgggggggtggGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATg                             <  5:186058/136‑1 (MQ=255)
                                                                                                   gggggggggtgggGTTTATTTGATCTGGCTGGGAATTAAACTATGGCGCTGCCCAGGCTTGCTGCGGTACCTGCCCGCGAACCGGGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATg                             <  3:169189/134‑1 (MQ=255)
                                                                                                   ggggggggggggggATTATTTCTATTGGCGGGGAATTCAATTCGGGCGCAGGCCAGGCCCGCCGCTGGAACTCCCCGCCCACCGCGCGCGGCGGGGCAATGCCGGGCTGTTTCGCCTGGGCCTGCTTTCGGCCATg                             <  6:16081/134‑1 (MQ=255)
                                                                                                    tgggtgggtgggGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATg                             <  5:263496/135‑1 (MQ=255)
                                                                                                    ggggtgggtgggGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGGCCTGGCCTGCAGCTGCAACTGACCGCGCACTGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATg                             <  3:229397/134‑1 (MQ=255)
                                                                                                    ggggtgggggggGCTTATTGGAGGGGGCGGGGCTTTCAACTATGGCGCCGGCCAGGCCTGCAGGTGGAAGTGACGGCGCACCGGGCGCGGCTGGGCAATGCCGGGCGGTTTCGCCAGGGCCTGCTTTCGGCCATgg                            <  5:144637/135‑1 (MQ=255)
                                                                                                        tgggtgggGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGaa                        >  5:195930/1‑136 (MQ=255)
                                                                                                          ggtgggGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAAcc                      >  2:284466/1‑136 (MQ=255)
                                                                                                             ggggTTTATTTGATGTGGCGGGGCTTTCAACTATGGCGCAGTCCAGGCCTGCTGCTGGAACTGACCGCGCACTGCGGGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCa                   <  5:279239/136‑1 (MQ=255)
                                                                                                              gggCTTATTTGATCTGGCTGGGCGTTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACCGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAAcccc                    >  6:268221/1‑134 (MQ=255)
                                                                                                              gggCTTATTTGATCTGGCTGGGCGTTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAAcccc                    >  6:273821/1‑134 (MQ=255)
                                                                                                              gggCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAAcccc                    >  7:123402/1‑134 (MQ=255)
                                                                                                                 cTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCTGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCAAGGt               >  7:52226/1‑136 (MQ=255)
                                                                                                                 cTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCAAGGt               >  7:1104/1‑136 (MQ=255)
                                                                                                                   tttttGATCTGGCGGGGCATTCAACTATGGCGAAGTCCTGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCAAGGtg              <  8:384635/133‑1 (MQ=255)
                                                                                                                   tATTTGATCTGGCTGGGCGTTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCAAGGtg              <  5:268221/135‑1 (MQ=255)
                                                                                                                   tATTTGATCTGGCTGGGCGTTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCAAGGtg              <  5:273821/135‑1 (MQ=255)
                                                                                                                   tATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCTGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCAAGGtg              <  1:107213/135‑1 (MQ=255)
                                                                                                                   tATTTGAGCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCGGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCAAGGtg              <  2:16201/135‑1 (MQ=255)
                                                                                                                    aTTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCAAGGtgct            >  4:400606/1‑136 (MQ=255)
                                                                                                                    aTTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCAAGGtg              <  5:201443/134‑1 (MQ=255)
                                                                                                                      ttGATGTGGTTGGGCATTAAAATATGGCGAAGGCCTGGCGTGCAGCTGTAACTGACCGCGCACCGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCAAGGtgctg           <  2:218064/135‑1 (MQ=255)
                                                                                                                              gctggGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCAAGGTGCTGCTGtt      <  6:81833/132‑1 (MQ=255)
                                                                                                                              gctggGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCAAGGTGCTGCTGTTCTAt  >  2:231168/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                                
CGTGGGCGGGGTCTTGGGTTTCAGCGCCCTCATCGCCTTGACCATGTTCGGCCTCAGCGCACTGCTCCAAACCTCGGCGGCAGTGCTGAGCGTCCTGAAGTGGGTGGGTGGGGCTTATTTGATCTGGCTGGGCATTCAACTATGGCGCAGTCCAGGCCTGCAGCTGGAACTGACCGCGCACAGCGCGCGGCTGGGCAATGCCGGGCTGTTTCGCCAGGGCCTGCTTTCGGCCATGGCGAACCCCAAGGTGCTGCTGTTCTAT  >  ACB143/4037604‑4037865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑