Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	4,263,673	C→T	5.4%	C117Y (TGC→TAC)	CJ019_03998 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	4,263,673	0	C	T	5.4%	250.0 / 2.3	73	C117Y (TGC→TAC)	CJ019_03998	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (19/50);  new base T (2/2);  total (21/52)
Fisher's exact test for biased strand distribution p-value = 5.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACAGGTCGGCGATGGTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCGGCAAGGTATTCGAG  >  ACB143/4263538‑4263806                                                                                                                                        |                                                                                                                                      gACAGGTCGGCGATGGTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGc                                                                                                                                       <  7:270359/136‑1 (MQ=255)   cAGGTCGGCGATGGTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGca                                                                                                                                      >  7:44785/1‑135 (MQ=255)   cAGGTCGGCGATGGTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCCGTGTTCTTCACGca                                                                                                                                      >  1:186940/1‑135 (MQ=255)         ggCGATGGTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGcaaaacaa                                                                                                                                >  1:175424/1‑135 (MQ=255)         ggCGATGGTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGcaaaacaa                                                                                                                                >  8:285674/1‑135 (MQ=255)         ggCGATGGTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGcaaaacaa                                                                                                                                >  7:96369/1‑135 (MQ=255)         ggCGATGGTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGAGTTCTTCAGGcaaaacaa                                                                                                                                >  8:111822/1‑135 (MQ=255)                gTCTACCGCCCACCGACCATGAATGGCGGATTCGCCAGGTTCGCGGCGACATTACGCTAAGCCGCCGTGGCACTCTCTCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAaccgca                                                                                                                        <  4:253865/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGGCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGTTGTTCTTCAGGCAAATCAAAAagcgca                                                                                                                        <  7:74640/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGTAAAACAAAAagcgca                                                                                                                        <  5:557/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAagcgca                                                                                                                        <  3:139669/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAagcgca                                                                                                                        <  6:316386/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAagcgca                                                                                                                        <  5:62218/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAagcgca                                                                                                                        <  3:269018/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAagcgca                                                                                                                        <  5:344863/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAagcgca                                                                                                                        <  5:175853/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAagcgca                                                                                                                        <  5:133915/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAagcgca                                                                                                                        <  5:163432/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAagcgca                                                                                                                        <  5:98724/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAagcgca                                                                                                                        <  2:58746/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAagcgca                                                                                                                        <  6:269494/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAagcgca                                                                                                                        <  7:229233/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAagcgca                                                                                                                        <  8:2837/136‑1 (MQ=255)                gTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAagcgca                                                                                                                        <  1:130517/136‑1 (MQ=255)                        gCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCACGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGcagcgcagcg                                                                                                                >  4:208224/1‑136 (MQ=255)                             cGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACAGCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTaa                                                                                                             >  8:289454/1‑134 (MQ=255)                               aCCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGc                                                                                                         <  4:243561/136‑1 (MQ=255)                                  aTGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGTAAAACAAAAAGCGCAGCGCAGCGTAATAGCTgg                                                                                                      >  7:333552/1‑136 (MQ=255)                                      ttGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGa                                                                                                   <  8:7087/134‑1 (MQ=255)                                      attgCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGAACTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGaa                                                                                                  >  7:7087/4‑136 (MQ=255)                                         gCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACt                                                                                                <  4:173737/135‑1 (MQ=255)                                          cGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGTAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTa                                                                                              >  2:348222/1‑136 (MQ=255)                                          cGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTa                                                                                              <  5:75997/136‑1 (MQ=255)                                             cTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATgg                                                                                           >  5:24130/1‑136 (MQ=255)                                             cTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATgg                                                                                           <  6:215967/136‑1 (MQ=255)                                               tCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGtt                                                                                         >  7:247670/1‑136 (MQ=255)                                                cGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGtt                                                                                         <  3:223822/135‑1 (MQ=255)                                                 gCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGt                                                                                       <  2:280537/136‑1 (MQ=255)                                                  ccAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTc                                                                                      <  7:285674/136‑1 (MQ=255)                                                               gACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAg                                                                         <  7:215871/136‑1 (MQ=255)                                                                        gTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGAtc                                                                <  3:72788/136‑1 (MQ=255)                                                                           aGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTcg                                                             <  6:143434/136‑1 (MQ=255)                                                                            gccgccGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTcgc                                                            >  7:104251/1‑136 (MQ=255)                                                                            gccgccGTGGCACGCTCGCGCAGGAACCGCGTCACATCGCATTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCAGTTGAACTAATGGTTGTCGACCAGCATCCAGCGCCATATCTcgc                                                            >  1:98059/1‑136 (MQ=255)                                                                                    ggCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTttca                                                    >  1:71964/1‑136 (MQ=255)                                                                                             gcgcAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGTAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTttcatcttcat                                             <  5:48062/134‑1 (MQ=255)                                                                                             gcgcAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTttcatcttcatca                                           >  1:161353/1‑135 (MQ=255)                                                                                             gcgcAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTttcatcttcat                                             <  6:122100/134‑1 (MQ=255)                                                                                             gcgcAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTttcatcttcat                                             <  7:163799/134‑1 (MQ=255)                                                                                                cAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCTTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGa                                        <  6:266068/136‑1 (MQ=255)                                                                                                 aGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAg                                       >  6:387221/1‑136 (MQ=255)                                                                                                     aCCGCGTCACATCGTGTTCGACGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCTCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGccc                                   <  7:354400/136‑1 (MQ=255)                                                                                                      ccGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGccc                                   >  7:256265/1‑135 (MQ=255)                                                                                                        gcgTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGaa                                >  6:118244/1‑136 (MQ=255)                                                                                                            cacaTCGGTTTCGCCGGTGTTCTTCAGGCAAAACaaaaa                                                                                                                             <  1:86611/39‑1 (MQ=255)                                                                                                            cacaTCGGTTTCGCCGGTGTTCTTCAGGCAAAACaaaaa                                                                                                                             >  2:86611/1‑39 (MQ=255)                                                                                                            cacaTCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTgg                            >  6:330543/1‑136 (MQ=255)                                                                                                            cacaTCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTgg                            >  8:217103/1‑136 (MQ=255)                                                                                                            cacaTCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTgg                            >  6:321129/1‑136 (MQ=255)                                                                                                            cacaTCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTgg                            >  6:386753/1‑136 (MQ=255)                                                                                                              caTCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGcc                          <  2:131295/136‑1 (MQ=255)                                                                                                                   gTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCt                     <  2:263803/136‑1 (MQ=255)                                                                                                                   gTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGAGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCt                     <  8:376883/136‑1 (MQ=255)                                                                                                                       cGCCGGTGTTCTTCAGGCAAAAAAATAAGCGCTGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGACCTGGCCGGTCTGtt                  <  6:17815/135‑1 (MQ=255)                                                                                                                        gCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCg                <  5:179558/136‑1 (MQ=255)                                                                                                                        gCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCg                <  8:44785/136‑1 (MQ=255)                                                                                                                        gCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCg                <  8:259251/136‑1 (MQ=255)                                                                                                                        gCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCg                <  2:186940/136‑1 (MQ=255)                                                                                                                        gCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCg                <  6:324925/136‑1 (MQ=255)                                                                                                                        gCCGGTGTTCTTCAGGCAAAACAAAAACCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCg                <  3:288049/136‑1 (MQ=255)                                                                                                                        gCCGGTGTTATTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCg                <  7:212323/136‑1 (MQ=255)                                                                                                                            gtgtTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCGGc              >  7:176861/1‑134 (MQ=255)                                                                                                                               ttcttcAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTttcatcttcat                                             >  2:226017/1‑100 (MQ=255)                                                                                                                               ttcttcAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTttcatcttcat                                             <  1:226017/100‑1 (MQ=255)                                                                                                                                    cAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCGGCAAGGTAt       <  5:387221/133‑1 (MQ=255)                                                                                                                                    cAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCGGCAAGGTAt       <  2:106452/133‑1 (MQ=255)                                                                                                                                     aGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCTTCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCGGCAAGGTATTCGa   <  1:369207/136‑1 (MQ=255)                                                                                                                                      ggCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCGGCAAGGTATTCGAg  <  6:315902/136‑1 (MQ=255)                                                                                                                                      ggCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCGGCAAGGTATTCGAg  <  5:176060/136‑1 (MQ=255)                                                                                                                                      ggCAAAACAAAAACCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCGGCAAGGTATTCGAg  <  5:281598/136‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      GACAGGTCGGCGATGGTCAACCGGCCACCGACCATGAATGGCGTCTTCGCCAGGTGCGCGTCGACAATACGGTAAGCCGCCGTGGCACGCTCGCGCAGGAACCGCGTCACATCGGTTTCGCCGGTGTTCTTCAGGCAAAACAAAAAGCGCAGCGCAGCGTAATAGCTGGTGAACTTATGGTTGTCGAACAGCATCCAGCGCCAGATCTCGCGCTTTTCATCTTCATCGCGAGGCCCGAACTGGCCGGTCTGTTCGGCAAGGTATTCGAG  >  ACB143/4263538‑4263806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑