Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,472,981 T→C 6.1% L418P (CTG→CCG)  hcaB_2 → 3‑phenylpropionate‑dihydrodiol/cinnamicacid‑dihy d rodiol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,472,9810TC 6.1% 222.2 / 4.2 68L418P (CTG→CCG) hcaB_23‑phenylpropionate‑dihydrodiol/cinnamicacid‑dihy d rodiol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (25/38);  new base C (2/2);  total (27/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

GTCTGGATGGGTAACGCGGTGGGGGATAAATCGCAGTCCCATAGCCGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAGACTACGTCCATGCC  >  ACB143/4472847‑4473110
                                                                                                                                      |                                                                                                                                 
gtCTGGATGGGTAACGCGGTGGGGGATAAATCGCAGTCCCATAGCCGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTg                                                                                                                                  >  2:6637/1‑136 (MQ=255)
    ggATGGGTAACGCGGTGGGGGATAAATCGCAGTCCCATAGCCGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTaa                                                                                                                              >  6:40823/1‑136 (MQ=255)
           tAACGCGGTGGGGGATAAATCGCAGTCCCATAGCCGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGgcg                                                                                                                        >  2:44023/1‑135 (MQ=255)
             aCGCGGGGGGGGATAAATCGCAGTCCCATAGCCGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCa                                                                                                                      <  7:39477/135‑1 (MQ=255)
                      gggATAAATCGCAGTCCCATAGCCGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTg                                                                                                            <  2:220667/136‑1 (MQ=255)
                      gggATAAATCGCAGTCCCATAGCCGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTg                                                                                                            >  4:41247/1‑136 (MQ=255)
                      gggATAAATCGCAGTCCCATAGCCGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGATAATAGGCGCAGCGGCGCTTg                                                                                                            >  2:380566/1‑136 (MQ=255)
                             aTCTCACTCCCATAGCCGAAACATCATGGGCCAACATGTTTTAGCTCGCATGGTACAGAGGCTGACTCGACGGGGTGTGTAGCAAACAAAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAatcat                                                                                                     <  6:101855/136‑1 (MQ=255)
                             aTCGCAGTCCCATAGCCGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAatcat                                                                                                     <  2:53139/136‑1 (MQ=255)
                              tcgatgTCCAATAGCCTAAACCTCATCGGCAAACCTGTTTGACCCTCAAAGTGACATAAGGTGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAatcatc                                                                                                    <  5:2074/131‑1 (MQ=255)
                              tCGCAGTCCCATAGCCGAACCCTCATCGGCCAACCTGTTTGAGCCTCCAAGTGCCAGAAGTAGTATCTACGGTGGGTGGAGGAAACCTAAAGATAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAatcatc                                                                                                    <  4:95077/136‑1 (MQ=255)
                                    tCCCATAGCCGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAggg                                                                                              >  4:362645/1‑136 (MQ=255)
                                        aTAGCCGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTaa                                                                                          <  8:260731/136‑1 (MQ=255)
                                            ccGAACCCTCTTCGGCCAACGTGTTTGAGCCGCCATGTGACACACGGAGTATCGACGGCGGGTGGAGGAAACCTAAAGATAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAAt                                                                                      <  4:357638/136‑1 (MQ=255)
                                            ccGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAAt                                                                                      <  1:51507/136‑1 (MQ=255)
                                            ccGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAAt                                                                                      <  6:164120/136‑1 (MQ=255)
                                            ccGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAAt                                                                                      >  3:95605/1‑136 (MQ=255)
                                                 aCCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCt                                                                                 >  1:109053/1‑136 (MQ=255)
                                                   cTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGt                                                                               >  7:227597/1‑136 (MQ=255)
                                                         gggCAACATGTTTGAGCCTCAAAGTGACAGAAGGAGTATCGACGGAGGGTGTAGTAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCg                                                                          <  7:243324/135‑1 (MQ=255)
                                                             aaCCTGTTTGAGCCTCCAAGTGACATACGGATTATCGACGGAGGGTGGAGTAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCa                                                                     <  1:131441/136‑1 (MQ=255)
                                                                   tttGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGaaa                                                                >  7:42515/1‑135 (MQ=255)
                                                                       acccTCCAAGTCACAGAAGGAGTAAGGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACt                                                            <  5:329446/133‑1 (MQ=255)
                                                                       aGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACt                                                            <  1:44023/135‑1 (MQ=255)
                                                                            ccAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAg                                                      >  1:129712/1‑136 (MQ=255)
                                                                             cAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGaaa                                                       <  7:161503/134‑1 (MQ=255)
                                                                              atttGACAGAATTATTATCTACGGTGGTTGGAGGAAACCTAAAGCTAAAACGCCACTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGc                                                    <  8:307183/133‑1 (MQ=255)
                                                                              aaGTGACCGAAGGTGTTTCGACGGAGGGTGGAGGAAACCTAAAGATAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGc                                                    <  2:29256/136‑1 (MQ=255)
                                                                              aaGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGc                                                    <  3:362645/136‑1 (MQ=255)
                                                                                gTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCaa                                                  >  2:21870/1‑136 (MQ=255)
                                                                                     aGACGGAGTAGCCCCGGCGGGTGGAGGAAACTTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCt                                             <  2:363383/136‑1 (MQ=255)
                                                                                     aGAAGGAGTATCCACGGCGGGTGGAGGAACCCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCt                                             <  3:294043/136‑1 (MQ=255)
                                                                                      gAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCGGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTa                                            >  2:291485/1‑136 (MQ=255)
                                                                                       aaGTAGTATCGAGGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCGACATCTAt                                           <  8:296291/136‑1 (MQ=255)
                                                                                       aaGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTAt                                           >  7:146155/1‑136 (MQ=255)
                                                                                          gAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAatcatc                                                                                                    <  2:48337/76‑1 (MQ=255)
                                                                                          gAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAatcatc                                                                                                    >  1:48337/1‑76 (MQ=255)
                                                                                                  aCGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGa                                >  7:171196/1‑136 (MQ=255)
                                                                                                      aGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGa                            <  1:6637/136‑1 (MQ=255)
                                                                                                        ggTGGAGGAAACCTAAAGCTAAAACGCCCCCGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATc                                              <  1:119955/116‑1 (MQ=255)
                                                                                                        ggTGGAGGAAACCTAAAGCTAAAACGCCCCCGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATc                                              <  1:125839/116‑1 (MQ=255)
                                                                                                        ggTGGAGGAAACCTAAAGCTAAAACGCCCCCGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATc                                              >  2:125839/1‑116 (MQ=255)
                                                                                                        ggTGGAGGAAACCTAAAGCTAAAACGCCCCCGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATc                                              >  2:119955/1‑116 (MQ=255)
                                                                                                           ggaggaAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTtc                       <  6:293824/136‑1 (MQ=255)
                                                                                                            gaggaAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTtct                      <  1:118216/136‑1 (MQ=255)
                                                                                                            gaggaAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTtct                      <  2:109053/136‑1 (MQ=255)
                                                                                                            gaggaAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTtc                       <  1:84835/135‑1 (MQ=255)
                                                                                                                 aaCCTAAAGCAAACACGCCCCAGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCaa                 <  1:171826/136‑1 (MQ=255)
                                                                                                                  aCCTAAAGCTAACACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCCGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAg                <  8:306912/136‑1 (MQ=255)
                                                                                                                  aCCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAg                <  3:392998/136‑1 (MQ=255)
                                                                                                                  aCCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAg                <  5:98815/136‑1 (MQ=255)
                                                                                                                  aCCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAg                <  8:320006/136‑1 (MQ=255)
                                                                                                                     tAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAatcatc                                                                                                    <  1:215053/49‑1 (MQ=255)
                                                                                                                     tAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAatcatc                                                                                                    >  2:215053/1‑49 (MQ=255)
                                                                                                                     tAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACCTCTATCGGGGTATGGACGGAGCTTCTCGCAAGAc              >  6:318734/1‑135 (MQ=255)
                                                                                                                     tAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCCAGAc              >  8:332300/1‑135 (MQ=255)
                                                                                                                     tAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAGAc              >  7:22135/1‑135 (MQ=255)
                                                                                                                     tAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAGAc              >  8:159229/1‑135 (MQ=255)
                                                                                                                       aaGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAGACt             <  7:124166/134‑1 (MQ=255)
                                                                                                                       aaGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAGACTAc           <  3:103818/136‑1 (MQ=255)
                                                                                                                        aGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAGACTACg          <  7:159229/136‑1 (MQ=255)
                                                                                                                        aGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAGACTACg          <  6:138763/136‑1 (MQ=255)
                                                                                                                          caaacaCGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAGACTACGTc        <  5:55721/131‑1 (MQ=255)
                                                                                                                               aCGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAGACTACGTCCATGc   >  7:65936/1‑136 (MQ=255)
                                                                                                                                cGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAGACTACg          <  4:316552/128‑1 (MQ=255)
                                                                                                                                cGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAGACTACg          >  3:316552/1‑128 (MQ=255)
                                                                                                                                 gCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAGACTACGTCCATGcc  >  8:138776/1‑135 (MQ=255)
                                                                                                                                 gCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAGACTACGTCCATGcc  >  3:53878/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                 
GTCTGGATGGGTAACGCGGTGGGGGATAAATCGCAGTCCCATAGCCGAAACCTCATCGGGCAACCTGTTTGAGCCTCCAAGTGACAGAAGGAGTATCGACGGAGGGTGGAGGAAACCTAAAGCTAAAACGCCCCTGCTAATAGGCGCAGCGGCGCTTGGAATCATCGCAGGGTTAACAATCGCCTGTTCCCGACGCAGGAAACACTAGAAAGGCAACATCTATCGGGGTATGGACGGAGCTTCTCGCAAGACTACGTCCATGCC  >  ACB143/4472847‑4473110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑