Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,729,452 C→T 5.5% V173M (GTG→ATG)  sdhA_2 ← Succinate dehydrogenase flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,729,4520CT 5.5% 244.0 / 3.5 73V173M (GTG→ATG) sdhA_2Succinate dehydrogenase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (20/49);  new base T (2/2);  total (22/51)
Fisher's exact test for biased strand distribution p-value = 5.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

GTTGGTGGTGGACGCGTAGATACGGCCCGCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGCCACCTTTAC  >  ACB143/4729318‑4729583
                                                                                                                                      |                                                                                                                                   
gTTGGTGGTGGACGCGTAGATACGGCCCGCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCAc                                                                                                                                     >  5:347311/1‑135 (MQ=255)
gTTGGTGGTGGACGCGTAGATACGGCCCGCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCAc                                                                                                                                     >  4:25307/1‑135 (MQ=255)
gTTGGTGGTGGACGCGTAGATACGGCCCGCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCAc                                                                                                                                     >  1:6923/1‑135 (MQ=255)
gTTGGTGGTGGACGCGTAGATACGGCCCGCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGACCTGGTTTTTCAc                                                                                                                                     >  4:258435/1‑135 (MQ=255)
gTTGGTGGTGGACGCGTAGATACGGCCCGCACCGCCAGTGGCCAGCACGGTGGCCTAGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCAc                                                                                                                                     >  7:347333/1‑135 (MQ=255)
 ttggttggGGACGCGTAGATACGGCCCGCACAGCCAGTGGCCAGCACGGTGTCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCa                                                                                                                                   <  5:359351/136‑1 (MQ=255)
 ttGGTGGTGGACGCGTAGATACGGCCCGCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCa                                                                                                                                   <  6:649/136‑1 (MQ=255)
     tggtggACGCGTAGATACGGCCCGCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGt                                                                                                                                >  4:214427/1‑135 (MQ=255)
             gcgTAGATACGGCCCGCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCgt                                                                                                                       <  5:76605/136‑1 (MQ=255)
               gTAGATACGGCCCGCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCgtag                                                                                                                     <  2:397817/136‑1 (MQ=255)
                       ggCCCGCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGtt                                                                                                             <  6:211416/136‑1 (MQ=255)
                         cccGCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGa                                                                                                           <  3:306159/136‑1 (MQ=255)
                          ccGCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGACCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAg                                                                                                          <  3:258435/136‑1 (MQ=255)
                            gCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAgg                                                                                                         >  1:329047/1‑135 (MQ=255)
                            gCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAgg                                                                                                         >  4:210133/1‑135 (MQ=255)
                                cGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGGAGTACTCGTTGAGGAACgt                                                                                                    >  6:56513/1‑136 (MQ=255)
                                   cAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACgtggt                                                                                                 <  6:183816/136‑1 (MQ=255)
                                              aCGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCa                                                                                      <  7:51401/136‑1 (MQ=255)
                                                ggTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAgg                                                                                    >  7:156440/1‑136 (MQ=255)
                                                ggTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAgg                                                                                    >  3:154109/1‑136 (MQ=255)
                                                  ttgCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGtt                                                                                  <  2:59441/134‑1 (MQ=255)
                                                  tGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGtt                                                                                  <  4:219451/136‑1 (MQ=255)
                                                  tGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGtt                                                                                  <  3:376384/136‑1 (MQ=255)
                                                  tGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGtt                                                                                  <  2:320451/136‑1 (MQ=255)
                                                  tGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGtt                                                                                  <  1:275217/136‑1 (MQ=255)
                                                  tGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGtt                                                                                  <  8:159103/136‑1 (MQ=255)
                                                  tGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTACTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGtt                                                                                  <  8:272075/136‑1 (MQ=255)
                                                  tGGCCTTGGACTTGATGTACAGGGGTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGtt                                                                                  <  1:268810/136‑1 (MQ=255)
                                                  gggCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGtt                                                                                  <  3:259256/135‑1 (MQ=255)
                                                    gCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGc                                                                                <  4:380604/136‑1 (MQ=255)
                                                    gCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGc                                                                                <  6:374358/136‑1 (MQ=255)
                                                     ccTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCATCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGcc                                                                               >  5:3168/1‑136 (MQ=255)
                                                     ccTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGcc                                                                               >  8:303064/1‑136 (MQ=255)
                                                     ccTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGcc                                                                               >  1:110342/1‑136 (MQ=255)
                                                     ccTTGGACTTGATGTACAGGGTTTCGCCGGGTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCATCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGcc                                                                               >  5:8126/1‑136 (MQ=255)
                                                         ggACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTg                                                                            <  7:297750/135‑1 (MQ=255)
                                                          gACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTgg                                                                           <  8:41148/135‑1 (MQ=255)
                                                          gACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTgg                                                                           >  2:3848/1‑135 (MQ=255)
                                                          gACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTgg                                                                           <  4:259744/135‑1 (MQ=255)
                                                          gACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGt                                                                          <  4:363703/136‑1 (MQ=255)
                                                            cTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTAc                                                                        <  2:70803/136‑1 (MQ=255)
                                                            cTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTAc                                                                        <  2:60813/136‑1 (MQ=255)
                                                                 tGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGgt                                                                   >  5:366370/1‑136 (MQ=255)
                                                                  gTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGgtg                                                                  >  3:35121/1‑136 (MQ=255)
                                                                  gTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGgtg                                                                  >  1:323812/1‑136 (MQ=255)
                                                                     cAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCATCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGtgtg                                                                <  6:3168/135‑1 (MQ=255)
                                                                     cAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCATCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTGGCCCTGGTACAGGGtgtg                                                                <  6:8126/135‑1 (MQ=255)
                                                                        ggTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTgcag                                                             >  5:291477/1‑135 (MQ=255)
                                                                         gTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTgcagca                                                           <  6:111424/136‑1 (MQ=255)
                                                                             cGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCa                                                                                      >  7:207334/1‑105 (MQ=255)
                                                                             cGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCa                                                                                      <  8:207334/105‑1 (MQ=255)
                                                                             cGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAgcgc                                                       >  3:382969/1‑136 (MQ=255)
                                                                             cGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAgcgc                                                       >  5:156514/1‑136 (MQ=255)
                                                                               ccGGTTTCGATGCAGATGGCGATCACACCCACGAACGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGt                                                     <  3:155799/136‑1 (MQ=255)
                                                                                     tCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCgg                                               >  7:359675/1‑136 (MQ=255)
                                                                                     tCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCgg                                               >  6:94267/1‑136 (MQ=255)
                                                                                         tGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACg                                           <  4:205020/136‑1 (MQ=255)
                                                                                            aGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTc                                        <  1:70930/136‑1 (MQ=255)
                                                                                                   gATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCg                                 <  3:25307/136‑1 (MQ=255)
                                                                                                   gATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCg                                 <  2:19974/136‑1 (MQ=255)
                                                                                                              cGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACg                      <  4:35121/136‑1 (MQ=255)
                                                                                                                 aTGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGggc                   <  7:61944/136‑1 (MQ=255)
                                                                                                                 aTGCGCCGTCCTGGGTTTTCACCATGGCAACTGCGTAGTCCTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGggc                   <  2:19073/136‑1 (MQ=255)
                                                                                                                      ccGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCt              <  2:323812/136‑1 (MQ=255)
                                                                                                                         tCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGGACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGc           <  1:3848/136‑1 (MQ=255)
                                                                                                                          ccTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCGCGCCTTCAGGtt                                                                                  <  7:52251/64‑1 (MQ=255)
                                                                                                                          ccTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGtt                                                                                  >  8:52251/1‑64 (MQ=255)
                                                                                                                          ccTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGc           >  2:300728/1‑135 (MQ=255)
                                                                                                                                   tccccAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGCCACCTTTAc  <  1:304551/132‑1 (MQ=255)
                                                                                                                                   tCACCAGGTCCACTGCGTAGTACTCGTTGAGGAACGGGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGCCACCTTTAc  <  1:105980/135‑1 (MQ=255)
                                                                                                                                   tCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGCCACCTTTAc  <  2:48598/135‑1 (MQ=255)
                                                                                                                                   tCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGCCACCTTTAc  <  2:324453/135‑1 (MQ=255)
                                                                                                                                   tCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGCCACCTTTAc  <  1:52172/135‑1 (MQ=255)
                                                                                                                                   tCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGCCACCTTTAc  <  4:279836/135‑1 (MQ=255)
                                                                                                                                   tCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGCCACCTTTAc  <  2:233123/135‑1 (MQ=255)
                                                                                                                                   tCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGCCACCTTTAc  <  1:213881/135‑1 (MQ=255)
                                                                                                                                   tCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGCCACCTTTAc  <  6:291477/135‑1 (MQ=255)
                                                                                                                                   tCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGCGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGCCACCTTTAc  <  4:281176/135‑1 (MQ=255)
                                                                                                                                   tCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGGGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGCCACCTTTAc  <  5:396028/135‑1 (MQ=255)
                                                                                                                                      ccAGGTCAACTGCTTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCt                                                                             <  2:200731/57‑1 (MQ=255)
                                                                                                                                      ccAGGTCAACTGCTTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCt                                                                             >  1:200731/1‑57 (MQ=255)
                                                                                                                                      |                                                                                                                                   
GTTGGTGGTGGACGCGTAGATACGGCCCGCACCGCCAGTGGCCAGCACGGTGGCCTTGGACTTGATGTACAGGGTTTCGCCGGTTTCGATGCAGATGGCGATCACACCCACGAATGCGCCGTCCTGGTTTTTCACCAGGTCAACTGCGTAGTACTCGTTGAGGAACGTGGTGCCCGCCTTCAGGTTGCCCTGGTACAGGGTGTGCAGCAGCGCGTGACCGGTACGGTCGGAAGCGGCACAGGTACGGGCGGCCTGGCCACCTTTAC  >  ACB143/4729318‑4729583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: