| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 5,507,090 | T→G | 53.3% | L35R (CTG→CGG) | CJ019_05179 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,507,090 | 0 | T | G | 53.3% | ‑4.5 / 4.1 | 13 | L35R (CTG→CGG) | CJ019_05179 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (3/2); new base G (4/2); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01 | |||||||||||
CCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCG > ACB143/5506960‑5507218 | ccAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGATGCGGCCGGCCCGcgag > 7:339934/1‑133 (MQ=255) tCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCAGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCAGCTCGAAGGCTGGgcag > 7:298421/1‑136 (MQ=255) tGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACACCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGCGGCGGCCGGCCGGCGGGGCGGCTGGGGCGCCCCACCCGCAGCCCCGCGCCTGCCgcc > 1:98592/1‑135 (MQ=255) cTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGGCGGGGCGGAGGCGGGGCCGCCCAACCCCATGCCGCGCCCCGGccccagc > 3:34745/1‑131 (MQ=255) cTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGACCCGGCCCGCCGGCTCCAAAGCAGGGCAGCACACCCCCCTCCCCCGCGCCGGccccccc > 3:231567/1‑131 (MQ=255) gctgctAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCGCGAAGGCTGGGCCGCCCAACCCCATGCCGCGCGCCTGCCGCGGCTGGAAAACCTg > 7:34820/1‑136 (MQ=255) acacccccTGCCAACCCCCGCCAGCGAGGGCGAATTCTTTCTCCCCGCCAAGCCCGCCTACGAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCaa < 5:357749/133‑1 (MQ=255) aTCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGACCAGGCCGGCCTGCTCGCACGGTGGGCCGCACACCCCCCGGCCGCCCCCCGGCCGCGGCGGGAGAACCAGCGCCaac > 1:149704/1‑135 (MQ=255) cccccTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCCGCCGGCCGGGGCGAAGGCGGGGGCGGCCCACCCGCTGCCCCCCGCCAGCCGCGGCGGGCAAAACAGCGcccacgc > 2:81993/1‑131 (MQ=255) aGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAACAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGAc > 4:25178/1‑135 (MQ=255) gCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCGGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACaa < 8:250652/136‑1 (MQ=255) tgctaaTTCTTTGTCCCCGCGCGGCCGCCCGCGCAGGCCGGCCGGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCa < 3:392650/132‑1 (MQ=255) gccgtcgTGTTCGAAGGCTGGGCAGCACAACCCTATGGAGCGCGCCTGCCGCTGCTGTAAAACCTGCGCCAAGGCCGCATCGCCACCAATGTCAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCg < 6:90979/136‑1 (MQ=255) | CCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCG > ACB143/5506960‑5507218 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |