| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 5,049,836 | (G)8→9 | coding (1335/1395 nt) | hcnB ← | Hydrogen cyanide synthase subunit HcnB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,049,828 | 1 | . | G | 83.3% | 83.1 / 12.5 | 30 | coding (1343/1395 nt) | hcnB | Hydrogen cyanide synthase subunit HcnB |
| Reads supporting (aligned to +/- strand): ref base . (0/5); new base G (16/9); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTCAGTCGCATAGCTGCCACCGTAAGTGGCATGCCCTTACGCGGCGGCCTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑A‑‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTCGCGATGGTGTAGCCGCACTGCCGACCTTGGCAGGGGCCCATACCTGCACGCGTAAACGCCTT > ACB143/5049695‑5049958 | ttcTCAGTCGCATAGCTGCCACCGTAAGTGGCATGCCCTTACGCGGCGGCCTCATAAGGTTTTTCAATTTATTAATCATGGCTATTTCTCCTCCACATCAAGTTAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑A‑‑gg < 3:306103/134‑1 (MQ=255) cTCAGTCGCATAGCTGCCACCGTAAGTGGCATGCCCTTACGCGGCGGCCTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAACTGTTATTGGTTTA‑A‑‑gggg > 6:26958/1‑136 (MQ=255) aGTCGCATAGCTGCCACCGTAAGTGGCATGCCCTTACGCGGCGGCCTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑A‑‑ggggg < 5:170772/134‑1 (MQ=255) cGCATAGCTGCCACCGTAAGTGGCATGGCCTTACGCGGCGGCCTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑A‑‑gggggggggc > 4:273826/1‑134 (MQ=255) aGCTGCCACCGTAAGTGGCATGCCCTTACGCGGCGGCCTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATc > 8:51049/1‑136 (MQ=255) aGCTGCCACCGTAAGTGGCATGCCCTTACGCGGCGGCCTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATc > 3:362489/1‑136 (MQ=255) gCCACCGTAAGTGGCATGCCCTTACGCGGCGGCCTCATCAGGTTTTTCAAGTTATGTATCATGGCTATTTCTCCTCCACATCATGTGAGGCCAGTTCCCCAAGTGTTTTTGGTTTA‑AG‑GGGGGGGGCGGATCCGGt < 2:307208/136‑1 (MQ=255) aCCGTAAGTGGCATGCCCTTACGCGGCGGCCTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATCCGGTAg > 2:244753/1‑135 (MQ=255) aCCGTAAGTGGCATGCCCTTACGCGGCGGCCTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATCCGGTAg > 8:112108/1‑135 (MQ=255) aCCGTAAGTGGCATGCCCTTACGCGGCGGCCTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATCCGGTAg > 3:269863/1‑135 (MQ=255) cccTTACGCGGCGGCCTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCaa > 4:37178/1‑136 (MQ=255) cccTTACGCGGCGGCCTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTACCCCAAGTGTTATTCGTTTA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCaa > 8:148536/1‑136 (MQ=255) ggacacatcaGTTTTTTCAAGTTATGAATCATGGCTAAGTCTCCTCCACATCCAGTGAGGCCAGTTCCCCATGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATCCGTTAGACACCAACATCCTCAACTGCTTTttgtt < 8:60244/131‑1 (MQ=255) ccTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGc > 5:107977/1‑136 (MQ=255) ccTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGc > 7:18191/1‑136 (MQ=255) ccTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGc > 3:231117/1‑136 (MQ=255) aaGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGc > 5:38236/1‑136 (MQ=255) gTTCTGAATCATGGCTATTTCTCCTCCCCATCACGGGAGGCCCGGTCCCCAAGTGTTCTTGGGTTACG‑‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTc < 5:19873/136‑1 (MQ=255) ttATGAATCCTGGCTATTTGTCCTCCACATCAAGTCTGGCCAGTTCCCCAAGTGTTGTTGGTTTA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTcg < 3:126487/136‑1 (MQ=255) ttAGGAATCATGGCTATTTCTCCTCCCCATCAAGTGAGGCCAGTTCCCCACGTGTTATTGGCTTACG‑‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTcg < 2:74459/136‑1 (MQ=255) cTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTCGCGATGGTGTAGcc > 8:120156/1‑136 (MQ=255) tcctccACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑AGGGGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGATCGGCTGCAACGATGCTCGCGATGGTGTAGCCGCACTg > 6:276166/1‑136 (MQ=255) accccaTCCAGTGAGGCCAGGACCACAACTGTAATTGGGTTA‑AG‑GGGGGGGGCGGATCAGGAAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGTCTGCAACGATGCTCGCGATGGTGTAGCCGCACTGCCGa < 1:345895/132‑1 (MQ=255) cacaTCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑AGGGGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTAGGCTGCAACGATGCTCGCGATGGTGTAGCCGCACTGCCGa > 3:305141/1‑135 (MQ=255) ctCAAGTGAGGCCAGTTCCCCAAGTGTGATTGGTTTA‑A‑‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTCGCGATGGTGTAGCCGCACTGCCGACCTTgg < 4:234434/135‑1 (MQ=255) aTCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTACG‑‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTCGCGATGGTGTAGCCGCACTGCCTACCTTg < 3:79018/136‑1 (MQ=255) tCAAGTGAGGCCAGGTCCCCAAGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTCGCGATGGTGTAGCCGCACTGCCGACCTTgg < 7:157863/136‑1 (MQ=255) cgacgggAGCCCAGGCCCCCACGGGTTCTTGGCTTA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTCGCGATGGTGTAGCCGCACTGCCGACCTTgg < 2:347027/130‑1 (MQ=255) cgAGGCCACTTCCCCAAGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTCGCGATGGTGTAGCCGCACTGCCGACCTTGGCAggg < 1:217711/135‑1 (MQ=255) gAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTCGCGATGGTGTAGCCGCACTGCCGACCTTGGCAgggg > 1:347027/1‑136 (MQ=255) gtgtTATTGGTTTA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTCGCGATGGTGTAGCCGCACTGCCGACCTTGGCAGGGGCCCATACCTGCAcgcg < 6:38236/136‑1 (MQ=255) gggtTATTGGTTCACG‑‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTCGCGATGGTGTAGCCGCACTGCCGACCTTGGCAGGGGCCCATACCTGCAcgcg < 8:18191/134‑1 (MQ=255) ggTTTA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTCGCGATGGTGTAGCCGCACTGCCGACCTTGGCAGGGGCCCATACCTGCACGCGTAAACGcc > 7:33127/1‑136 (MQ=255) tttA‑AG‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTCGCGATGGTGTAGCCGCACTGCCGACCTTGGCAGGGGCCCATACCTGCACGCGTAAACGCCtt < 5:93258/136‑1 (MQ=255) | TGCTCAGTCGCATAGCTGCCACCGTAAGTGGCATGCCCTTACGCGGCGGCCTCATCAGGTTTTTCAAGTTATGAATCATGGCTATTTCTCCTCCACATCAAGTGAGGCCAGTTCCCCAAGTGTTATTGGTTTA‑A‑‑GGGGGGGGCGGATCCGGTAGAAACCAACATCCTCAACTGCTTTTTGTTGCTCGGCTGCAACGATGCTCGCGATGGTGTAGCCGCACTGCCGACCTTGGCAGGGGCCCATACCTGCACGCGTAAACGCCTT > ACB143/5049695‑5049958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |