| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 5,386,513 | +AA | intergenic (‑522/+1129) | CJ019_05070 ← / ← nptII | hypothetical protein/neomycin phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,386,512 | 1 | . | A | 91.7% | 35.1 / ‑2.1 | 12 | intergenic (‑521/+1130) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (8/3); total (8/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
| * | ACB143 | 5,386,512 | 2 | . | A | 91.7% | 33.5 / ‑2.1 | 12 | intergenic (‑521/+1130) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (8/3); total (8/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
ATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGT‑CA‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG > ACB143/5386435‑5386605 || aTCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg < 7:193529/135‑1 (MQ=255) tCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTa > 4:348148/1‑136 (MQ=255) aTTAAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGGGGTGTACGGTAC‑‑AACCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 6:189997/136‑1 (MQ=255) atatGACATGTCGGCCGGCTTGTCGACGCCGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACTCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 5:350171/136‑1 (MQ=255) atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 2:323315/136‑1 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 1:257975/1‑136 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 2:164309/1‑136 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 2:269571/1‑136 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 8:192406/1‑136 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg > 4:338331/1‑136 (MQ=255) gccggcTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCCCCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg > 5:93592/1‑136 (MQ=255) gccggcTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCGTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTTCCCTTggg > 3:206195/1‑136 (MQ=255) || ATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGT‑CA‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG > ACB143/5386435‑5386605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |