Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,038,1970AC33.3% 94.1 / 10.6 54V380G (GTG→GGG) sotB_2sugar efflux transporter
Reads supporting (aligned to +/- strand):  ref base A (2/34);  new base C (16/2);  total (18/36)
Fisher's exact test for biased strand distribution p-value = 1.00e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGCGCTGCCGCTTGGCCGATAGCGGGCCCGCGAACACGGGCGAAGCCCGTGCCCTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTCATGTACACCGCATTCAGCCGCCCGCGGCTGGCCGGGTCCAGTGC  >  ACB143/4038066‑4038327
                                                                                                                                    |                                                                                                                                    
ggCGCTGCCGCTTGGCCGATAGCGGGCCCGCGAACACGGGCGAAGCCCGTGCCCTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACC‑CCCGGCAACCCCGcccccccccc                                                                                                                                  >  1:32187/1‑134 (MQ=255)
                 gATAGCGGGCCCGCGAACACGGGCGAAGCCCGTGCCCTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTgccag‑c                                                                                                               <  8:244990/136‑1 (MQ=255)
                 gATAGCGGGCCCGCGAACACGGGCGAAGCCCGTGCCCTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTgccag‑c                                                                                                               <  2:272646/136‑1 (MQ=255)
                 catcgCGGGCCCGCCAACACGGGCCATGCCCGTGCCCTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTgccag‑c                                                                                                               <  3:4726/132‑1 (MQ=255)
                           ccGCGAACCCGGGCGACGCCCGTGCCCTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCt                                                                                                     <  3:18659/136‑1 (MQ=255)
                           ccGCGAACACGGGCGAAGCCCGTGCCCTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGcg                                                                                                     <  7:267283/136‑2 (MQ=255)
                                       gagAAGCCGGTGCCCTGCACCGCGTAACGGTGCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACt                                                                                         <  3:140116/134‑1 (MQ=255)
                                       gCGAAGCCCGTGCCCTTCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCACGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGTCTGAACAGCGCACt                                                                                         <  7:351913/136‑1 (MQ=255)
                                       gCGAAGCCCGTGCCCTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACt                                                                                         <  6:152812/136‑1 (MQ=255)
                                       gCGAAGCCCGTGACCTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACTCATCCAGCGCCACA‑CCCGTGAACCGCGCCCCCACCAGT‑TCCACGCCTTTCCAG‑ACAAACTGGCTGAACAGCGCACt                                                                                         <  8:329905/136‑1 (MQ=255)
                                              ccGTGCCCTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCAcc                                                                                  <  3:8939/136‑1 (MQ=255)
                                              ccGTGCCCTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCAcc                                                                                  <  3:353850/136‑1 (MQ=255)
                                                gTGCCCTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACC‑CCCGGCCACCCCGCCCCCCCCAGT‑GCCCCGCCATGCCCG‑CCAAACAGGCGGAACAGCGCCCAGGCcccccc                                                                                >  7:166027/1‑134 (MQ=255)
                                                gTGCCCTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCCCCCGG‑GCCCCCCCTTGCCAG‑CCCAACAGGCTGAACAGCGCCCTGGCcccccc                                                                                >  7:166023/1‑134 (MQ=255)
                                                    ccTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCCCA‑CCCGGCCCCCCCGCCCCCCCCCCG‑GCCCCCCCATGCCCG‑CCCAACAGGCGGAACAGCGCACAGGccccccccccc                                                                            >  2:371938/1‑133 (MQ=255)
                                                    ccTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCCCA‑CCCGGCAACCCCGCCCCCCCCCGT‑GCCCCGCCGTGCCAG‑CCCAACAGGGGGAACAGCGCACAGGCCCccgccgcc                                                                            >  1:214670/1‑134 (MQ=255)
                                                        cACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCa                                                                        <  1:362884/136‑1 (MQ=255)
                                                           cgcgTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCCCCCCT‑GGCCCGCCTTGCCCG‑CCAAACTGGCGGCACAGCCCACGGGCCCCCCCCGCCCCCcgcg                                                                     >  7:48254/1‑136 (MQ=255)
                                                           cgcgTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAgcg                                                                     <  6:306609/136‑1 (MQ=255)
                                                               tAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGcccc                                                                 <  2:268183/136‑1 (MQ=255)
                                                                aaCGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACACCCAGCGCCCCA‑CCCGGCACCCCCGCCCCCACCCCG‑GCCCCCCCTTGCCCGCCCAAAGTGG‑TGAAAACCGCCCAGGCCCCCCCCGCCCCCCGCGccccc                                                                >  4:176639/1‑135 (MQ=255)
                                                                aaCGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCCCA‑CCCGGCCACCCCGCCCCCCCCCGT‑GCCCCGCCTTGCCAC‑CCAAACGGGCTGTACAGCGCCAAGGCCCCCGCCCACCCCCCCGccccc                                                                >  5:22767/1‑135 (MQ=255)
                                                                aaCGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACC‑CCCGGCAACCCCGCCCCCCCCCCG‑GCCCCCCCATGCCAG‑CCCAACAGGCGGAACACCGCCCCGGGCCCCGCCCGCCCCCGCGCCccg                                                                >  2:21882/1‑136 (MQ=255)
                                                                aaCGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACACCCCGCCAACCCCGCCCCCCCCCGT‑G‑CACGCCATTCCCG‑CACAACGTGCGGAAAAACCCACAGGCCCCCGCCCACCCCAGCGCCccg                                                                >  2:78224/1‑136 (MQ=255)
                                                                aaCGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCCACCCCCCCCCCCCCACT‑GCCCCCCCATGCCAC‑CCAAACAGGGGGAACAGCCCCCCGGCCCCCCCCCCCCCCCGccccccc                                                                >  3:296807/1‑135 (MQ=255)
                                                                aaCGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCCCCCCT‑GCCCCCCCATGCCCG‑CCCAAATGGCAGAACAGCCCACGTGCCACCGCCGCCCCCCGCGccccc                                                                >  8:344268/1‑135 (MQ=255)
                                                                aaCGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCCGCAACCCCCCCCCCCCCCCT‑GCCACCCCATGCCAA‑CCCAACAGGCAGCACACCGCACAGGCCCCCCCCCccccccgccccccg                                                                >  5:286236/1‑136 (MQ=255)
                                                                        aaCCCCGGCGTGCTTTGAGTAGAAACACAACCCGCGCCACA‑CCCGGCCCCCCCCCCCCCCCCCCTGGCCACCCATTCCCCC‑CCAAA‑AGGCGGCACACCGCACAGGCCCCCCCCCCCCCCCCCGCCCCCCCGCggg                                                         >  6:269532/1‑134 (MQ=255)
                                                                          ccccGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑GCCGGCAACCCCGCGCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGGCGACCCCAGCGGCCGGCCGAGGAAGa                                                      <  5:265614/136‑1 (MQ=255)
                                                                          ccccGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGa                                                      <  1:344645/136‑1 (MQ=255)
                                                                          ccccGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGa                                                      <  2:76942/136‑1 (MQ=255)
                                                                          ccccGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGa                                                      <  3:214006/136‑1 (MQ=255)
                                                                          ccccGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGa                                                      <  6:259521/136‑1 (MQ=255)
                                                                            ccGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATg                                                    <  3:287179/136‑1 (MQ=255)
                                                                            ccGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATg                                                    <  3:284709/136‑1 (MQ=255)
                                                                            ccGGCGTGCGTTGAGTAGAAACACAACCAGTGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATg                                                    <  5:71/136‑1 (MQ=255)
                                                                                cGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCCCCCCCCCCCCG‑GCCCCGCCGTTCCCC‑CCAAACAGGCGGCAACGCCCCCGGGCCCCCCCCCCCCCCCCCGCCCCCCCCCGGAAGAttcggg                                                >  6:73364/1‑131 (MQ=255)
                                                                                       tGAGTAGAAACACAACCAGCGCCCCC‑CCCGGCAACCCCCCCCCCCCCCCT‑GCCCCCCCCTTCCAC‑CCAAACATGCGGAAAAGCGCCCAGGCCCCCCCCGCCCCCCCCCCCCCCCCCCGGAAGATGCGGGTGATGTa                                         >  8:149915/1‑136 (MQ=255)
                                                                                       tGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTCATGt                                          <  3:273831/135‑1 (MQ=255)
                                                                                       tGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTCATGt                                          <  5:169863/135‑1 (MQ=255)
                                                                                                     aCCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTCATGTACACCGCATTCAgcc                           <  1:38522/136‑1 (MQ=255)
                                                                                                        aGCGCCACA‑CCCGGCAACCCCGCCCCCCCCAGT‑GCCCCGCCATTCCAG‑CCAAACAGGCTGAACAGCCCCCTGGCCACCGCCGCCCCCCGCCCCCCCCCGAGGAAGATGCTGGTCATGTTCACCGCAATCAgccgcc                        >  4:26264/1‑136 (MQ=255)
                                                                                                           gcCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTTGTCATGTACACCGCATTCAGccgcccgc                     <  8:224999/136‑1 (MQ=255)
                                                                                                               ca‑cCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTCATGTACACCGCATTCAGCCGCCCGCGGCt                 <  8:14861/136‑1 (MQ=255)
                                                                                                                    cGGCAACCCCGCCCCCATCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTCATGTACACCGCATTCAGCCGCCCGCGGCTGGcc             >  1:131955/1‑136 (MQ=255)
                                                                                                                       cAACCCCGCCCCCCCCCGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTAATGTACACCGCATTCAGCCGCCCGCGGCTGGCCggg          <  2:327099/136‑1 (MQ=255)
                                                                                                                       cAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTCATGTACACCGCATTCAGCCGCCCGCGGCTGGCCggg          <  5:73364/136‑1 (MQ=255)
                                                                                                                       cAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTCATGTACACCGCATTCAGCCGCCCGCGGCTGGCCggg          <  2:120354/136‑1 (MQ=255)
                                                                                                                       cAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTCATGTACACCGCATTCAGCCGCCCGCGGCTGGCCggg          <  7:314848/136‑1 (MQ=255)
                                                                                                                       cAACCCCGCCCCCACCAGG‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGCTGCTGGTCATGTACACCGCATTCAGCCGCCCGCGGCTGGCCggg          <  6:206723/136‑1 (MQ=255)
                                                                                                                        aaCCCCGCCCCCCCCAGT‑GCCCCGCCTTTCCAC‑CCAAACTGGCTGAACAGCGCACTGGCCACCCCCGCCCCCACCGCCCCGCCCAGGAAGATGCGGGGGATGTACACCGCAATCAACCGCCCCCGGCTGGCCGGGt         >  4:143024/1‑136 (MQ=255)
                                                                                                                          ccccGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTCATGTACACCGCATTCAGCCGCCCGCGGCTGGCCGGGTcc       <  2:149954/136‑1 (MQ=255)
                                                                                                                          ccccGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTCATGTACACCGCATTCAGCCGCCCGCGGCTGGCCGGGTcc       <  8:270484/136‑1 (MQ=255)
                                                                                                                              gCCCCCCCCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTCATGTACACCGCATTCAGCCGCCCGCGGCTGGCCGGGTCCAGTg   <  4:191573/136‑1 (MQ=255)
                                                                                                                               cccccACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTCATGTACACCGCATTCAGCCGCCCGCGGCTGGCCGGGTCCAGTGc  <  1:164261/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
GGCGCTGCCGCTTGGCCGATAGCGGGCCCGCGAACACGGGCGAAGCCCGTGCCCTGCACCGCGTAACGGTTCAACCCCGGCGTGCTTTGAGTAGAAACACAACCAGCGCCACA‑CCCGGCAACCCCGCCCCCACCAGT‑GCCACGCCTTGCCAG‑CCAAACTGGCTGAACAGCGCACTGGCCACCGCCGACCCCAGCGCCCCGCCGAGGAAGATGCTGGTCATGTACACCGCATTCAGCCGCCCGCGGCTGGCCGGGTCCAGTGC  >  ACB143/4038066‑4038327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑