Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,245,1280AC33.9% 36.6 / 12.6 61L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (12/25);  new base C (14/5);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 4.78e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAG  >  ACB143/4244999‑4245250
                                                                                                                                 |                                                                                                                          
ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCCGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGgcgc                                                                                                                      >  3:113222/1‑136 (MQ=255)
ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACGTCGCTGCCCAACCCGGCGGCCCGGgcgc                                                                                                                      >  2:16198/1‑136 (MQ=255)
    cgcgTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATc                                                                                                                  >  8:167729/1‑136 (MQ=255)
                gCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGCTCGGCGCCCCACaa                                                                                                      >  2:48495/1‑136 (MQ=255)
                    ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCAACCCGGCGGCCCGGGGGCGCTCGGCGCCCCCCAACacg                                                                                                  >  4:195860/1‑136 (MQ=255)
                        tGCGCATTGGCGATGGGCGGGCAATGGGCCAGGCGCCCGGCCAGTTCGGCGGAAACGGCAGGAAACTGCTCGTCGGCGCAGAGTTCGCTGACCAACCCGGCGGCCTGGGCGCTATCGGCGCCCCACAACTCGTCGa                                                                                              <  4:89782/136‑1 (MQ=255)
                          cgcATAGGCGAAGGGGGGGCCATTGGCCTGGCGCCCGGCCAGTTCGGGGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACAAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACtcgtcg                                                                                               <  1:365168/133‑1 (MQ=255)
                             aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGACCAACCCGGCGGCCAGGGCGCGCTCGGCGCCCCACAACTCGTCGAGGaa                                                                                          >  3:244082/1‑135 (MQ=255)
                             aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCCACTCGACGAGGaa                                                                                          >  8:282502/1‑135 (MQ=255)
                             aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGCTCGGCGCCCCACAACTCGGCGAGGaa                                                                                          >  6:242424/1‑135 (MQ=255)
                             aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGCggta                                                                                          >  2:234457/1‑133 (MQ=255)
                             aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAc                                                                                         <  7:245223/136‑1 (MQ=255)
                              tAGGCGATGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTCCTCGTCGGCGAAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                        <  2:25762/136‑1 (MQ=255)
                              tAGGCGATGGGCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCACCGGCAGGCAACGGCTCGTCGGCGTAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTAGAGGAAca                                                                                        <  4:297388/136‑1 (MQ=255)
                              tAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTGGGCGGCAACTGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                        <  2:253237/136‑1 (MQ=255)
                              tAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                        <  7:187362/136‑1 (MQ=255)
                               aGGCGATGGTCGGGCCTTTGGCCTGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                        <  3:60350/135‑1 (MQ=255)
                               aGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTGCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                        <  5:107685/135‑1 (MQ=255)
                               aGGCGAAGGGCGGGCCATTGGCCAGGCGCCGGGCCTGTTCGGCGGCAAGGGCAGGCAACTGCTGGGGGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGGGCGATGGGCGCCCCACAACTCGTCGAGGAAca                                                                                        <  1:359126/135‑1 (MQ=255)
                                  cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAcagcag                                                                                    >  4:50761/1‑136 (MQ=255)
                                       gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACGAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCt                                                                               >  3:276711/1‑136 (MQ=255)
                                       gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCGCTCGGCGGCCCCCCACTCGTCGAGGCAAAGCCgggcct                                                                               >  3:69958/1‑132 (MQ=255)
                                       gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCAACGCGTCGGGGAACCGAAGgcgcg                                                                               >  7:327905/1‑135 (MQ=255)
                                       gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACCACTCGTCGAGGAACAGCAGGCGCt                                                                               >  2:10323/1‑136 (MQ=255)
                                       gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCt                                                                               >  1:71325/1‑136 (MQ=255)
                                        gcGGGCCTTTGGCCTGGCGCCCGGCCTGGGGGGCGGCAACGGCAGGCAACTGCGCGTCGGCGCAGACTTCGCTCACCAACCCGGCGCCCAGGGCGCTCTCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCtt                                                                              <  5:97489/135‑1 (MQ=255)
                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCACCACGGCGCGGGACACCCCGCGCGTgggcgg                                                                        >  5:28079/1‑132 (MQ=255)
                                                    ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCGATCGGCGCCCCCCAACACGGCGAGGCCCCCCCGGCGCTTGGCCGGCTCg                                                                    >  4:54905/1‑134 (MQ=255)
                                                       ggCGCCCGGCCTGTTCGGCGGCAACGGCAGGAAACTGCTCGTCGGGGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                               <  4:252419/136‑1 (MQ=255)
                                                       ggCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                               <  3:71930/136‑1 (MQ=255)
                                                       ggCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                               <  2:29013/136‑1 (MQ=255)
                                                        gcgcCCGGCCAGTTCGGCGGAAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                               <  7:54141/135‑1 (MQ=255)
                                                        gcgcCCGGCCAGTGCGGGGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                               <  1:51943/135‑1 (MQ=255)
                                                         cgcCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCCCGATCCGCGCCCCACACCTCGGCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGa                                                             >  8:22740/1‑136 (MQ=255)
                                                            cgggCCAGTTCGGCGGCAACGGCAGGCAACGGCTCGGCGGCGCATACTGCGCTGACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCa                                                          <  6:347170/134‑1 (MQ=255)
                                                                       ggcggcAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGACCACCCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGGGGGAgg                                               >  8:255457/1‑136 (MQ=255)
                                                                       ggcggcAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCAACTCCTGGAGGGACCGCAGGCGCGTGGCCTGCTCGCTGCCGGTCAGCCGTgggcgg                                               >  7:318249/1‑132 (MQ=255)
                                                                       ggcggcAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACGCGTCGCGGAACAGCAGGCGCTTGGCCTGCTCGCTGGCGCTCAGCCGTGGCAgg                                               >  5:141161/1‑136 (MQ=255)
                                                                         cggcAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCGATCGGCGCCCCCCCCCAAGTCGAGGAACACCCCGCGCATGGCCTGCTCCCGGCCGAACAGCCGGGGCacggg                                             >  3:191766/1‑132 (MQ=255)
                                                                            cAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCa                                          <  7:91927/136‑1 (MQ=255)
                                                                                  caggcaACTGCTCGGCGGCGCAGACTTCGCTGGCCCACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                    <  8:96741/136‑1 (MQ=255)
                                                                                  caggcaACTGCGCGTCGGCGCAGACTTCGCTCCCCACCCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                    <  6:19752/136‑1 (MQ=255)
                                                                                  caggcaACTGCGCGCCGGCGCAGACTTCGCGGCCCACCCCGGCGGCCCGGGCGCGAGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                    <  5:309151/136‑1 (MQ=255)
                                                                                       accTGCGCGCCGGCGCATACTGCCCTCACCACCCCGGCGGCCCGGGCGCGAGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCgg                               <  6:28079/134‑1 (MQ=255)
                                                                                       aaCTGCGCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCgg                               <  4:120288/136‑1 (MQ=255)
                                                                                         cTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                             <  4:277848/136‑1 (MQ=255)
                                                                                         cTGCTCGGCGGCGCATACTGCGCTGACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                             <  1:217226/136‑1 (MQ=255)
                                                                                          tGCTGGGCGGCGCAGACTGCGCTGACCACCCCGGCGGCCAGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCAt                            <  1:322337/136‑1 (MQ=255)
                                                                                               gtcgGCGCAGACTTCGCTGCCCACCCCGGCGGCCAGGGCGGGAGCGGCGCCCCACAACTCGTGGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                       <  7:265666/136‑1 (MQ=255)
                                                                                                 cgGCGCATAGTGCGCTGCCCACCCCGGCGGCCGGGGCGCGAGCGGGGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCt                     <  4:161310/136‑1 (MQ=255)
                                                                                                  ggCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAAc                                                                                                     >  4:303317/1‑55 (MQ=255)
                                                                                                  ggCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAAc                                                                                                     <  3:303317/55‑1 (MQ=255)
                                                                                                    cgcAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGGTGGGCGGCCCCCAAAGAGGCGAGGCACCGCCGGCTCTTTGCCGGGTGCCAGCCGATCAGGCGGGGGAGGTGCCACCTGGCGCCGGCCTCgcggggg                    >  6:106503/1‑131 (MQ=255)
                                                                                                    cgcAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTg                    >  3:206877/1‑134 (MQ=255)
                                                                                                        gcattCGCTCACCACCCCGGGGGCCTGGGCGGTATGGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc              <  4:95308/133‑1 (MQ=255)
                                                                                                                 gACCAACCCGGCGGCCCCGGCGCGATCGGCGCCCCCCAACTCGTCGAGGAACAGCAGGCGCTTGGCCAGCTCGCTGCCGCTAAGCCGTGGCAGGGGCCAGCTGGCGCCGGCATCGGGGCTGTCGCCCCTGCTGgtg     >  5:364177/1‑136 (MQ=255)
                                                                                                                  cccACCCCGGGGGCCCGGGGGCGTTCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt    <  7:177361/135‑1 (MQ=255)
                                                                                                                  accaccCGGGGGGCCTGGGGGCGTTCGGCGCCCCAGAACTCGTCGAGGAACTGCTGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt    <  6:323836/136‑1 (MQ=255)
                                                                                                                  acaaaCCCGGCGGCCAGGGCGCGTTCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt    <  1:328256/133‑1 (MQ=255)
                                                                                                                  aCCAACCCGGCGGCCTGGTCGCGAGCGGCGCCCGACAACTGGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt    <  2:218648/136‑1 (MQ=255)
                                                                                                                    ccccccGGCGGCCAGGGGGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAg  <  2:363588/133‑1 (MQ=255)
                                                                                                                                 |                                                                                                                          
CCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAG  >  ACB143/4244999‑4245250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑