Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,287,8210GT28.9% 82.1 / 11.7 46intergenic (+143/‑82)CJ019_04021/gloAhypothetical protein/Lactoylglutathione lyase
Reads supporting (aligned to +/- strand):  ref base G (17/15);  new base T (13/0);  total (30/16)
Fisher's exact test for biased strand distribution p-value = 1.82e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCTGCCTGGCGTCACCGCGCAACCTGA  >  ACB143/4287689‑4287952
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acaTAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTgcg                                                                                                                                   >  8:192609/1‑135 (MQ=255)
       ctccaccCTTTTTCCCCACGGACTGGCGCCGTATCCAACCCGATAAAGCCCTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGgc                                                                                                                           <  5:102433/133‑1 (MQ=255)
       cTTCACATTTTTTACACACGGATGGGCCCATTACCCAACCACATAACGCAATCGCGACCCCACCCCGCGCCCGCACTTGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCCGGGCGCTTTCATTTGCGCAATCGgc                                                                                                                           <  4:170741/136‑1 (MQ=255)
       cTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGGGCCCAGCCGTTTCAGGGGGCGTTGATTTTAGCAAtacgc                                                                                                                           >  4:13932/1‑132 (MQ=255)
          cacaCTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCATTGCATGGCGCTTTAATTTTCGCAATACGCGCa                                                                                                                        >  1:84941/1‑136 (MQ=255)
           acacTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCGTTTCAGGGCGCGTTAATTTTCGCAATCGGCGCa                                                                                                                        >  7:219519/1‑135 (MQ=255)
            ccattttttCACACGGACTGGATAAGTACCCAAACCGAAAACGCAATCGCCACCCCATCCCGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACt                                                                                                                      <  8:6613/133‑1 (MQ=255)
                     cacaAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCAGCCATTTAAGGGCGCGTTAATTTTCGCAATAGGCGCACTGGCGTAta                                                                                                              >  4:376958/1‑134 (MQ=255)
                                   cAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGGGCGTTCATTTTGGCCATCGGCGCCCTGCCGTATCCTGGCCAGCcggtgt                                                                                               >  5:75201/1‑134 (MQ=255)
                                       ctCGAAACCGCTAACGCACTAGCGACCCCAGCCCGCGCCCGCACATGCGGGCCGCAAATTTCCGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGttt                                                                                           <  6:304016/135‑1 (MQ=255)
                                         cGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTTCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGttttt                                                                                         >  3:93835/1‑135 (MQ=255)
                                         cGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCGTTGCAGGGCGCGTTCATTTTCGCCATCGGCGCCCTGCCGGATCATGGCCAGCCGGGCTGTTTTg                                                                                         >  3:238960/1‑136 (MQ=255)
                                          caaccAGATAACGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTGCAGCGCCCTGCCTTTGCCGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGc                                                                                        <  6:310264/132‑1 (MQ=255)
                                                   aaaGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGGCTTTGCAGGGCGCTTTCATTTGGGCAATCGGCGCACTGCCGTATCCTGGCCAGCCGGGAGGGTTTGCCGgcgcacg                                                                               >  2:190650/1‑133 (MQ=255)
                                                   aaaGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTTCAGGGCGCTTTCATTTTCGCAATCGGCGCACTGCCGTATAATGGCCAGCCTGTCTGTTTTTCCGGCGcccg                                                                               >  3:41930/1‑135 (MQ=255)
                                                   aaaGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTAATTTTCGCAATCGGCGCCCTGCCGGATCATGGCCCGCCTGTCTGTTTTGCCGGCGcccg                                                                               >  4:263068/1‑135 (MQ=255)
                                                           cccccccccAACCCGCGCCCGCACTTGCGGGCCTATAATTTCAGCCCCCGGCCTTTGCTGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCgg                                                                       <  5:114138/131‑1 (MQ=255)
                                                             cGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGcc                                                                     >  7:255596/1‑136 (MQ=255)
                                                              gACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTTCACGGCGGGTTGATTTGCGCAAACGGCGCCCAGCCGTATAATGGCCCGCCTGGCTGTTTTTCCGGCGCCCTGcgccccgccc                                                                    >  3:165624/1‑136 (MQ=255)
                                                                    aTCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGtct                                                               >  1:206372/1‑135 (MQ=255)
                                                                    aTCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCGGCCTTTGGAGGGGGCGTTGATTTTGGCAAAAGGCGCACTGCCGGATCATGGGCCGCCTGTGTTTTTTTCCGGCGCCCTGCgccccgcccccgcg                                                               >  6:114138/1‑131 (MQ=255)
                                                                    aTCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCAGCCTTTGCAGGGCGCGTTGATTTTCGCAATAGGCGCACTGCCGTATAATGGCCCGCCGGTCTGTTTTTCCGGCGCCCAGCGCCCGGCCCCctct                                                               >  5:68101/1‑135 (MQ=255)
                                                                     tCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCa                                                             <  8:255596/136‑1 (MQ=255)
                                                                     ccccgcgCCCCAACTTGCGGGCCTCTAATTACCGCGCCGTCCTTTGGCGGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCa                                                             <  7:134342/133‑1 (MQ=255)
                                                                       ccgccCCCGCACTTGGGGGCCTATAATTTCAGCGCCCTGCCTTTGCTGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAgg                                                           <  6:68101/135‑1 (MQ=255)
                                                                       aCGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCAGCCTTTGAAGGGCGCGTTCATTTTCGCAATCGGCGCACTGCCGGATCATGGGCACCCTGTGAGTTTTGCCCGCGCCCTGCGCCCCGCCCCGtagcctg                                                           >  8:63555/1‑130 (MQ=255)
                                                                            cccGCACATGCGGGCCGCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTc                                                      <  5:142503/136‑1 (MQ=255)
                                                                             ccGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTcc                                                     >  6:85066/1‑136 (MQ=255)
                                                                             ccGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATAGGCGCACAGCCGTATCATGGCCCGCCTGTCGGTTTTGCCGGCGCCCTGCGCCCGGCCCCCTGTCAGGAGTTcc                                                     >  5:151592/1‑136 (MQ=255)
                                                                                        ggCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTg                                           >  7:89458/1‑135 (MQ=255)
                                                                                        ggCCTATAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGc                                          <  6:317444/136‑1 (MQ=255)
                                                                                        ggCCGCTAATTTCCGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGc                                          <  8:69417/136‑1 (MQ=255)
                                                                                         gCCTCTAATTTCCCCGCCCGGCCTTTGCAGGGCGCTTTCATTTCCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCa                                         <  7:239234/136‑1 (MQ=255)
                                                                                                        gcCCTGCCTTTGCAGGGCGCGTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCCGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGGCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACcct                          >  1:214229/1‑136 (MQ=255)
                                                                                                          ccTGCCTTTTGAGGGCGCGGTCATTTTGGCAATAGGCGCCCTGCCGTATCATGGCCAGCCGGTGTGTTTTTCCGGCGCCCGGCGCCCGGGCCCGTCGCAGGAGTTGCCATGAGCCTGCCCGCTCTGGACACccggc                        >  4:339443/1‑136 (MQ=255)
                                                                                                                     aGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCTGCCTGGCGTCAcc             <  2:84941/136‑1 (MQ=255)
                                                                                                                       ggggcTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCTGCCTGGCGTCAcc             <  2:38153/131‑1 (MQ=255)
                                                                                                                           cTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCTGCCTGGCGTCACCGCGCaa       >  6:246899/1‑136 (MQ=255)
                                                                                                                           cTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCTGCCTGGCGTCACCGCGCaa       >  5:108121/1‑136 (MQ=255)
                                                                                                                           cTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCTGCCTGGCGTCACCGCGCaa       >  8:191863/1‑136 (MQ=255)
                                                                                                                           cTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCTGCCTGGCGTCACCGCGCaa       >  8:209667/1‑136 (MQ=255)
                                                                                                                           cTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCTGCCTGGCGTCACCGCGCaa       >  1:291608/1‑136 (MQ=255)
                                                                                                                           cTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGACTCTATTGCCGGCGACCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATCAGCCTGCACGATCTGCAAACCCTGCCTGGCGTCCCCGCGCaa       >  2:52665/1‑136 (MQ=255)
                                                                                                                            tttCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCTGCCTGGCGTCACCGCGCAAc      >  3:306340/1‑136 (MQ=255)
                                                                                                                            tttCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCTGCCTGGCGTCACCGCGCAAc      >  1:64792/1‑136 (MQ=255)
                                                                                                                              tCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTATGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCTGCCTGGCGTCACCGCGCAAc      >  4:185572/1‑134 (MQ=255)
                                                                                                                                 tttGCGCAATCGGCGCAGTGCCGTATCATGGCCAGCCTGTGTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCTGCCTGGCGTCACCGCGCAACCTGa  <  1:330967/135‑1 (MQ=255)
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ACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAAACCCTGCCTGGCGTCACCGCGCAACCTGA  >  ACB143/4287689‑4287952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑