Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,667,0820GT35.2% 52.4 / 17.3 54L161L (CTC→CTA) chrRQuinone reductase
Reads supporting (aligned to +/- strand):  ref base G (16/19);  new base T (19/0);  total (35/19)
Fisher's exact test for biased strand distribution p-value = 4.38e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGCCCCCG  >  ACB143/4666965‑4667215
                                                                                                                     |                                                                                                                                     
cGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCACACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTc                                                                                                                      >  6:326181/1‑135 (MQ=255)
          caGGGGCGTTGCACAGCAGGATTAGGGTTTCAGACCGCCCTGATCAACTTCAACCATGACCCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTcg                                                                                                           <  1:374909/136‑1 (MQ=255)
           aGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTcgc                                                                                                          >  5:263919/1‑136 (MQ=255)
                cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTGTTTTTTTTTATCTTTCCCGTATACTGGAAAAAGCTagccgccc                                                                                                     >  5:40155/1‑134 (MQ=255)
                cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCCTTTAGCTTGGCCGAATCCTCGCACAAGCTCGCCGCAc                                                                                                     >  1:97267/1‑136 (MQ=255)
                cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGCACAAGCTcgccgccc                                                                                                     >  3:63063/1‑134 (MQ=255)
                cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCAc                                                                                                     <  1:191905/136‑1 (MQ=255)
                cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCAATTAGCTTTCCCGAATCCTCGAACAAGCTcgcccccc                                                                                                     >  5:290964/1‑132 (MQ=255)
                cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGGCTTGTCATTTAGGATGCCCCAATCCTCGAACAAGCAcgccgccc                                                                                                     >  5:44366/1‑134 (MQ=255)
                cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGGCTTGTCATTGAGCTTTCCCGACTCCTCGCACCAGCTCGCCGCAc                                                                                                     >  4:361197/1‑136 (MQ=255)
                cGTTGCACAGCAGGATTAGGGGTTCACACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCAc                                                                                                     <  5:50192/136‑1 (MQ=255)
                 gTTGCACAGCAGGATTAGGGGTTCCGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCAcc                                                                                                    <  2:173846/136‑1 (MQ=255)
                 gTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCAcc                                                                                                    <  7:126338/136‑1 (MQ=255)
                  ttGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTGGTGTCATTGAGCTTGGCCGAATCCTCGAACAAGCTAGCCGCACCg                                                                                                   >  7:163054/1‑136 (MQ=255)
                     cacaGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATCACGCAAACCTCTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTAAATGAGCATTCCCGAAAACTACAACACGCTATCCGCACCGcc                                                                                                 >  5:365592/1‑135 (MQ=255)
                     cacaGCAGGATTAGGGGTTCAGACCGCCCGGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTGTTGTCATTTAGCTTGGCCGGATCCTCGAACAAGCACGCCGCACCGcc                                                                                                 >  1:73850/1‑135 (MQ=255)
                          cagGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGt                                                                                            >  5:162520/1‑135 (MQ=255)
                           agGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGGGTTGTGATTTAGCTTGGCCGCATCCTCGCACAAGCACGCCGCCCCGCCCAGGTa                                                                                           >  5:12454/1‑135 (MQ=255)
                            ggATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTGGTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTAGCCGCACCCCCCAGGTAAg                                                                                         >  3:300741/1‑136 (MQ=255)
                             gATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTTCCCGAATCCTCGCACAAGCTCGCCGCCCCGCCAAGGTAAGc                                                                                        >  2:321810/1‑136 (MQ=255)
                             gATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGc                                                                                        >  5:181339/1‑136 (MQ=255)
                             gATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGGGTTTTCATTTAGCTTGCCCGACTCCTCGGACAAGATCGCCGCCCCGCCCAGGTaatc                                                                                        >  5:226156/1‑134 (MQ=255)
                             gATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGGGTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGc                                                                                        >  1:102604/1‑136 (MQ=255)
                             gATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGGCTTGTACTTGAGCTTGCCCGAATCCAAGCACAATCTCGCCGCACCCCCCACGTAAGc                                                                                        >  4:106670/1‑136 (MQ=255)
                                 aGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTc                                                                                     <  5:5335/135‑1 (MQ=255)
                                 aGGGCTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTc                                                                                     <  5:5336/135‑1 (MQ=255)
                                  ggggTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCgg                                                                                   <  3:149929/136‑1 (MQ=255)
                                     gTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACCAGCTCGCCGCACCGCCCAGGTAAGCCTCGGGCa                                                                                >  8:211808/1‑136 (MQ=255)
                                          gACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCAATTAGCTTGCCCGAATCCTCGCACAACCTCGCCGCCCCGCCAACGTAAGCCTCGGGCaacag                                                                            >  2:159356/1‑131 (MQ=255)
                                           aCCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTGTTTTCATTTAGCTTGCCCGAAAACTCGCAAAACCTCCCCGCACCGCCAACGTAAGCCACGGGCATCtgc                                                                           >  6:274956/1‑135 (MQ=255)
                                                  tGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAggg                                                                   >  3:157772/1‑136 (MQ=255)
                                                    ttCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGc                                                                  <  3:306074/135‑1 (MQ=255)
                                                      cAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATg                                                               >  5:180813/1‑136 (MQ=255)
                                                      cAACTACACCCATGACGCAAACCTGTCGACAAACGCCTGCAACAACGGTCGCGTATTGTCAATGAGCTAGCCCGAATCCTCGAACAAGCACGCCACACCGCCAAGCTAAGCCTAGGGCAAAAGCAAGCAGGGCaag                                                               >  1:287691/1‑134 (MQ=255)
                                                            cACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCga                                                           <  3:277089/134‑1 (MQ=255)
                                                                 aTGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTGATTTAGCTTGCCCGAATCCTCGAACAAGCACGCCGCACCCCCCACGTAAGCCACCGGCAACTGCATGCAGGGCATGTCGAGAAacac                                                    >  1:33785/1‑136 (MQ=255)
                                                                 aTGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCCCCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAacac                                                    >  5:92450/1‑136 (MQ=255)
                                                                 aTGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAATCCTCGGGCATCTGCATGCAGGGCAAGTCGAGAAacac                                                    >  1:324403/1‑136 (MQ=255)
                                                                 aTGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAacac                                                    >  8:42948/1‑136 (MQ=255)
                                                                 aTGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAAACCACCCAAAAGCTAGCCGCACCCCCAACGTAAGCCACGGGCATATGCAAGCAGGGCATGTCGAGAAaccc                                                    >  1:32840/1‑134 (MQ=255)
                                                                          aCCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTg                                           <  1:52536/136‑1 (MQ=255)
                                                                          aCCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTg                                           <  8:163283/136‑1 (MQ=255)
                                                                            cTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGgc                                         >  1:25806/1‑136 (MQ=255)
                                                                                  aCAAACGCCTGCAAGAACGGTCGCGTGTTGTCATTGAGCTTGCCCGCATACTCGAACAAGCACGCCGCCCCCCCAACGTAACCCTCGGGCAACTGCAAGCAGGGCAAGTCGAGCAACCCCCGCCACAGGCGCAccc                                   >  6:289433/1‑135 (MQ=255)
                                                                                  aCAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCAGAAAACACGAACAACCTCGCCGCCCCGCCAACGTAAGCCTCCGGCATCAGCAAGCAGGGCATTTCTCGAAACCCCCGCGAATGGCGCAccc                                   >  7:245893/1‑135 (MQ=255)
                                                                                     aaCGCCTGCAAGAACGGTCGCGGCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCAt                                <  6:61764/136‑1 (MQ=255)
                                                                                       cGCCTGCAAGAACGGTCGCGGGTTGTCATTTAGCTTGCCCGAATCCGCGCACAACCTCGCCGCCCCCCCCAGGTAAGCCACGGGCCTCTGCAAGCACGGCATGTCGCGAAACACCAGCCCCAGGCGCCCCGCCTgg                              >  4:195816/1‑136 (MQ=255)
                                                                                                    ggTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGc                 <  6:181339/136‑1 (MQ=255)
                                                                                                       cgcgTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGccac              <  2:33785/136‑1 (MQ=255)
                                                                                                       cgcgTCTTGTCATTGAGCTTGCCCGAAGCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGGTGGCGCCAAAGccac              <  5:263304/136‑1 (MQ=255)
                                                                                                               gTCATTGAGCTTGCCCGAATCCTCGAACACGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGc       <  4:95892/135‑1 (MQ=255)
                                                                                                               gTCATTGAGCTTGCCCGAATCCTCGAACAAGATCGCCGCACCGACAAGGTAAGCCTCGGGCATATGCATGCAGGGCATTTCGAGAAACACCAGCGACTGGCGCACCGAATGGTTGGCGCCAAAGCCACCAATCGc       <  6:198119/135‑1 (MQ=255)
                                                                                                                tCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGccc     <  7:78691/136‑1 (MQ=255)
                                                                                                                   ttGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGCCCCCg  >  3:69626/1‑136 (MQ=255)
                                                                                                                     |                                                                                                                                     
CGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGCCCCCG  >  ACB143/4666965‑4667215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑