Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	2,113,897	0	C	G	41.7%	51.6 / 15.1	36	S664* (TCA→TGA)	CJ019_02007	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/20);  new base G (15/0);  total (16/20)
Fisher's exact test for biased strand distribution p-value = 2.87e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGCTCGGTCGTCGCTGAGGGCGATGGCAGGCGTTGCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATT  >  ACB143/2113770‑2114030                                                                                                                                |                                                                                                                                      ggCTCGGTCGTCGCTGAGGGCGATGGCAGGCGTTGCACCTATACCGCAGGCAGTAAGGGGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTAtg                                                                                                                               <  4:351288/136‑1 (MQ=255)              cTGAGGGCGATGGCAGGCGTTGCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTCCAATCTATGTGCTGGTACGCCa                                                                                                                  <  8:25982/136‑1 (MQ=255)              cTGAGGGCGATGGCAGGCGGTGCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGTTCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCa                                                                                                                  <  4:258222/136‑1 (MQ=255)                    gCGATGGCAGGCGTTGCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGAATCTGTGTGGTGGTTCGCCAgcggcg                                                                                                            >  1:200238/1‑136 (MQ=255)                        tGGCAGGCGTTGCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGAATCTATGTGCTGGTTCGCCAGCGGCGGccg                                                                                                        >  1:62062/1‑135 (MQ=255)                                gatGCACCTATACAGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGACCCGGGAGACCATCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGt                                                                                                <  8:135859/134‑1 (MQ=255)                                   gCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGa                                                                                              <  4:250035/135‑1 (MQ=255)                                       cTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGAATCTATTTGCTGGTACGCCAGCGGCGGCCTGAACTGGGGATCgcc                                                                                         >  2:240140/1‑134 (MQ=255)                                            ccGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGCACCGGGAGACCAGCGAGTCGCGGTGAATCTAGTTGGTGGTTCGCCACCCGCGGGCTGAACGGGGGGTCGCCGTc                                                                                      >  3:132654/1‑134 (MQ=255)                                                 ggcagTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGAATCTATTTGGTGGTTCGGCCGCGGGGGCCTGGACTGGTGATCGACGTGAAACAGt                                                                               >  2:7276/1‑136 (MQ=255)                                                  gcagTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGAATCTATTTGGTGGTACGCCAGCGGCGGCCGGAACTGGTGATCGACGTCAAACAGtt                                                                              >  6:240722/1‑136 (MQ=255)                                                  gcagTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGAATCTATTTGCTGGTAAGCCAGCGGCGGCCTGGACTGGTGATGGACGTCAAGCAGtt                                                                              >  7:32956/1‑136 (MQ=255)                                                    agTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTGAATCATAGGGGTGGGTCGCCCCCGGCGGGCGGCACTGGTGATAGGCGGCCAGCACTTGc                                                                            >  2:124598/1‑136 (MQ=255)                                                    agTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGAATCTATGTGCGGGGACGCCAGCGGCGGCCGGGAAGGGTGGTGGCCCTCAAGCAGTTgg                                                                            >  2:31820/1‑135 (MQ=255)                                                       aaGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTGTATATATTTGGTGGTGAGCCCGCCGCGGGCTGCACATGTGGTCGCCGGGAAACAGtttcgcg                                                                         >  7:325573/1‑131 (MQ=255)                                                             tcttccAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGAGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGc                                                                    <  4:122562/131‑1 (MQ=255)                                                               tGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGAATCTATTTGGTGGTTCGCCCGCGGCGGGCGGGAAGGGTGATCGACGTCAAGCAGTTTCCCGCTGGCggc                                                                 >  6:22486/1‑134 (MQ=255)                                                                 ccAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTGAATATATTTGGTGGTTCGCCCGCGGGGGCCGGCACTGGGGATGGACGTCAAGCAGTTGCCCGCTGGCGGccg                                                               >  5:244705/1‑135 (MQ=255)                                                                 ccAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCt                                                               <  3:161572/136‑1 (MQ=255)                                                                 ccAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCt                                                               <  5:196201/136‑1 (MQ=255)                                                                      gTGGCCTACGTGCTCGATCAGGTCCTGGTACAGAACCGGCAGACCAGCTAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTTATCGACGTAAAGCAGTTGCACGATGGCGACCTGCAGt                                                          <  6:214960/136‑1 (MQ=255)                                                                      gTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGt                                                          <  3:358052/136‑1 (MQ=255)                                                                      gTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGt                                                          <  3:373185/136‑1 (MQ=255)                                                                      gTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGt                                                          <  5:22486/136‑1 (MQ=255)                                                                           cTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACa                                                     <  6:258711/136‑1 (MQ=255)                                                                             aCGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGGTGAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATAGACCTCAAACAGTTGCCCGATGGCGACCTGCAGTTGACAgg                                                   >  7:185591/1‑136 (MQ=255)                                                                                     gATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGtt                                             <  5:240722/134‑1 (MQ=255)                                                                                      aTCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTGAAAATATTTGCTGGTACGCCACCGGCGGCCTGAACAGGTGATCGACGTAAAGCAGTTTGCCGATGGCGACCAGCAGTTGACAGGTATGt                                              >  1:8597/1‑132 (MQ=255)                                                                                              aggGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTa                                   <  7:264058/135‑1 (MQ=255)                                                                                                    cAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGgccag                             <  6:284577/135‑1 (MQ=255)                                                                                                       aaCCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGccagccag                         <  1:245553/136‑1 (MQ=255)                                                                                                        aCCGGGAGACCAGCGAGTCGCGTTCAAAAAATGTTCTGGTACGCCACCGGCGGCCGGGACTGGGTATAGAAGTCAAACAGTTTCCCCATTGGGACCGGCAGTTGAAAGGTATGTTCGTAGGGGTAGGCCAACCAGa                        >  2:93703/1‑136 (MQ=255)                                                                                                        aCCGGGAGACCAGCGAGTCGCGGTGAATATATGTGCTGGTAAGCCACCGGCGGCCTGAACTGGGGATCGACGTCAAGCAGTTGGCCGATGGCGCCCTGCAGTTGACAGGTATGTTCGATGGGGGAGGCCAGCCAGa                        >  4:114341/1‑136 (MQ=255)                                                                                                           gggAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGacac                     <  5:63861/136‑1 (MQ=255)                                                                                                                              ttCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAAtt  <  5:119458/136‑1 (MQ=255)                                                                                                                              ttCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAAtt  <  2:172678/136‑1 (MQ=255)                                                                                                                                |                                                                                                                                      GGCTCGGTCGTCGCTGAGGGCGATGGCAGGCGTTGCACCTATACCGCAGGCAGTAAGGTGGATGCCAACGTGGCCTACGTGCTCGATCAGGTCAGGGTACAGAACCGGGAGACCAGCGAGTCGCGTTCAATCTATGTGCTGGTACGCCAGCGGCGGCCTGAACTGGTGATCGACGTCAAGCAGTTGCCCGATGGCGACCTGCAGTTGACAGGTATGTTCGATGGGGTAGGCCAGCCAGACACCCAGTGGGCATTGCCAATT  >  ACB143/2113770‑2114030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑