Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,138,3500TG34.7% 48.7 / 13.5 49F240V (TTT→GTT) rbsC_2Ribose import permease protein RbsC
Reads supporting (aligned to +/- strand):  ref base T (12/20);  new base G (17/0);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 8.98e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCT  >  ACB143/3138220‑3138477
                                                                                                                                  |                                                                                                                               
cacGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGt                                                                                                                            >  7:66346/1‑136 (MQ=255)
        tCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCggcggc                                                                                                                    >  8:110349/1‑136 (MQ=255)
         cGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTATGTGCGGCAGCCTGGCCGCGGTTTGTTGGGAGGcc                                                                                                                   >  6:22532/1‑136 (MQ=255)
           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTGTTGTTGGGCGGCCTg                                                                                                                 >  7:200654/1‑136 (MQ=255)
           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTCGGCGGCCTg                                                                                                                 >  3:188417/1‑136 (MQ=255)
           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGGGGGCTg                                                                                                                 >  3:263690/1‑136 (MQ=255)
           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGccgg                                                                                                                 >  1:11348/1‑134 (MQ=255)
           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGccgg                                                                                                                 >  7:345080/1‑134 (MQ=255)
           gCCTGCGTCTGTACGCCATCGGAGAATACACGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTCCCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGccgg                                                                                                                 >  5:169088/1‑134 (MQ=255)
                cGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGTCCGCGTTTTGTTCGGCGTCCTGGTTc                                                                                                             <  6:55602/135‑1 (MQ=255)
                  tCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGGGGGCTGGGTGAGc                                                                                                          >  3:200300/1‑136 (MQ=255)
                      tACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCgg                                                                                                        <  2:140671/134‑1 (MQ=255)
                      tACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCAGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGgc                                                                                                       <  6:162208/135‑1 (MQ=255)
                                gagaATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCgg                                                                                            <  1:283179/136‑1 (MQ=255)
                                gagaATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCgg                                                                                            <  6:356118/136‑1 (MQ=255)
                                gagaATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTCGGGGGGCGGGTTGAGCGGCCGCcccccccg                                                                                            >  4:63241/1‑134 (MQ=255)
                                     tACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCg                                                                                       <  2:95610/136‑1 (MQ=255)
                                       cccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGc                                                                                     <  2:13802/136‑1 (MQ=255)
                                        ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGGCTGGTTCAGCGGCAGCACCACCGGGTCCGGCg                                                                                    >  5:119815/1‑136 (MQ=255)
                                           gAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTGTAGGCGGCCAGGGTGAGCGGCCGCACCCCCCGGTCCGGCgcgc                                                                                 >  2:312646/1‑133 (MQ=255)
                                            aGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAt                                                                                <  1:51836/136‑1 (MQ=255)
                                              gcggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTGGGCGGCCGTGTTCAGCGGCAGCACCCCCCGGTACGGCgctattg                                                                              >  7:134187/1‑130 (MQ=255)
                                              gcggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGGCTGGTTCAGCGGCCGCCCCACCGGTTCCGGCGGGCtgc                                                                              >  3:171933/1‑136 (MQ=255)
                                               cggcTAATGCCGCTGGCCTGACGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtgct                                                                             <  2:214552/136‑1 (MQ=255)
                                                ggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTGGGTCAGCGGCCGCCCCACCGGGTCCGGCGGGctgctg                                                                            >  5:181529/1‑136 (MQ=255)
                                                  cTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTGTGGTGGGGGGGGAGGTTAAGCAGACCCGCCACCGGGTCCGGGGAGAtggtggt                                                                          >  4:276078/1‑134 (MQ=255)
                                                     aTGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGGTCATCGGCAGCAACCCCGGGTCCGGCGAGCTGCTGCTGTc                                                                       >  4:103043/1‑136 (MQ=255)
                                                     aTGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGGTCGGCGGGCTGGTTGAGCGGCAGCCCCACCGGGTGCGGGGCGATGCTGCGGTc                                                                       >  4:108341/1‑136 (MQ=255)
                                                         cGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTGGTTGAGCGGCAGCACCCCACGTTGCGGCGAGATGCTGCTGTCCggg                                                                   >  2:102925/1‑134 (MQ=255)
                                                              gCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTGGGCGGCCTGGTTCAGCGGCAGCCCCCCCGGTTCCGGCGAGATGCAGCTTTGCGgggtggg                                                              >  7:165045/1‑135 (MQ=255)
                                                              gCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTAAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTGCGTGGGGGc                                                              >  5:239889/1‑136 (MQ=255)
                                                              gCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTGGTTCAGCGGCAGCCCCCCCGGGTGCGGCGAGATGCTGCGGTGCGGGGGGGc                                                              >  3:99764/1‑136 (MQ=255)
                                                                   tCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCTGCGAGATGCTGCTGTCCGTGGTGGCCATc                                                          <  4:297719/135‑1 (MQ=255)
                                                                       tGCGTGGCTACGTGTTCGCCAGTTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCttt                                                     <  4:42035/136‑1 (MQ=255)
                                                                       tGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCttt                                                     <  2:272892/136‑1 (MQ=255)
                                                                                aCGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCGGGGTCAGCGGCAGCCCCCCCGGTTCCGGCGAGATGCTGCTGTCCGGGGTGGCCATCGCTTTTCTTGGGGt                                            >  2:47716/1‑136 (MQ=255)
                                                                                 cGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGt                                            <  5:23118/135‑1 (MQ=255)
                                                                                    gtTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGAtg                                        >  7:102947/1‑135 (MQ=255)
                                                                                      tCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGGCGGGGTGAGCCGCCGCCCCCCCGGGTCCGGCGGGATGCTGCTGGACGTGGTGGGCCTCGCGTTTTTTTGGGGGAtctt                                      >  6:53260/1‑136 (MQ=255)
                                                                                         ccAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTTTTGGGCGGCCTGGTTCAGCGGCAGCACCCCCGGTTCCGGGGAGATGCTGCTGTCCCTGGTGGCCATCGCGTTTATTGGGGTGATAGtctc                                   >  8:297109/1‑136 (MQ=255)
                                                                                           aGCTACCTGATCAGCGGGCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTccc                                 <  8:257055/136‑1 (MQ=255)
                                                                                                ccTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGt                            <  8:153110/136‑1 (MQ=255)
                                                                                                ccTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTAGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATTCTGCTGTGCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGt                            >  7:80156/1‑136 (MQ=255)
                                                                                                  tGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTAGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCt                          >  5:187043/1‑136 (MQ=255)
                                                                                                    aTCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTg                         <  7:97853/135‑1 (MQ=255)
                                                                                                                   ggCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATcggcg         <  4:247573/136‑1 (MQ=255)
                                                                                                                     cAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGGTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCggcggc       <  2:97441/136‑1 (MQ=255)
                                                                                                                            gCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACgct  <  5:80850/134‑1 (MQ=255)
                                                                                                                            gCCGAGTTTTGTTCGTCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACgct  <  6:235715/134‑1 (MQ=255)
                                                                                                                                  |                                                                                                                               
CACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCT  >  ACB143/3138220‑3138477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑