Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,588,8880CA29.2% 108.3 / 14.1 72A21S (GCG→TCG) CJ019_03404hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/26);  new base A (0/21);  total (25/47)
Fisher's exact test for biased strand distribution p-value = 2.06e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CACCCTTGCCTCCGGCTAACACTTCCCCTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCTTCAGGCCCATTGGCGTAATCGAACTTCGCCCGGTGCT  >  ACB143/3588784‑3589021
                                                                                                        |                                                                                                                                       
cacCATTCCCTCCGGAAAACACTTCCCCTTGCCGGGTGTGTAGAGGAATTTCACCTCAAAGTCACCCATTTGCCCACCACAACCAAGCGGGTTGCGATTACGCGAAACGCGCCATGCCTGGCGCACACAAAAAACg                                                                                                          <  5:67959/136‑1 (MQ=11)
     ttGCCTCCGGCTAACACTTCCCCTTGCCGGGTGTGTAGAGGCCTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCGTACGCGCAACGCCCCCAGCCTGGCCCACACAAAAAACCGgccc                                                                                                     >  5:257255/1‑134 (MQ=11)
         cTCCGGCTAACACTTCCCCTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTg                                                                                                  >  7:355080/1‑135 (MQ=38)
           ccGGCTAACACTTCCCCTGCCCGGGTGTGTATAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACACCCACGCGGGTTGCGCTTACGCGAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCaa                                                                                               <  8:216352/136‑1 (MQ=14)
                tAACACTTCCCCTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAgcg                                                                                          >  1:256614/1‑136 (MQ=255)
                    acTTCCCCTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGCCCCCCACTTGGCCACCACACCCAAGCGGGTGGCGCTAACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCg                                                                                    <  1:369795/136‑1 (MQ=17)
                      ttCCCCTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGtt                                                                                  >  2:210636/1‑136 (MQ=255)
                          ccTTGCCGGGTGTGTAGAGGACTTTCACCGCCAAGTCACCCACTTGCCCACCACAACCAAGCGGGTTGCGCTTACGCGAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTg                                                                              <  5:54460/136‑1 (MQ=255)
                          ccTGGCCGGGGGTGTCTCGGCCTTCCCCCCCCACGCCACCCACTGGGCCCCCACACCCACGCGGGTGGGGCTTACGCCAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTg                                                                              <  4:240822/136‑1 (MQ=12)
                           cTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGGGCTTTCGCGCAACCCCCCCAGCCTGGCGCCCACAAAAAAACGGCGCCGGCAACAGCGCCCCGttttt                                                                               >  5:308682/1‑135 (MQ=11)
                           cTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTg                                                                              >  3:255763/1‑135 (MQ=255)
                           cTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTg                                                                              >  6:160910/1‑135 (MQ=255)
                           cTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTGTTTg                                                                              >  1:16086/1‑135 (MQ=255)
                               ccGGGGCTGTAGAGCACTTGCACCTCCAAGTCACCCACTTGCGCACCAAACCCACGCGGGGTGCGCTTACGGTAAACGCGCCATGCCTGGCGCACAGAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCa                                                                         <  8:43734/136‑1 (MQ=18)
                                 gggTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTAAGCGCAACGCGCCAAGCCGGGCGCACACAAAAAACGGGCGCCGGCAAGAGCGCCCGGTTTTGCCTCCAg                                                                       >  3:214561/1‑136 (MQ=17)
                                      tgtAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTg                                                                   <  7:179419/135‑1 (MQ=255)
                                       gtAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATAAAGCCTGCt                                                                 >  2:70042/1‑136 (MQ=255)
                                       gtAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTGTTTGCAACAAGCCTGCt                                                                 >  3:275834/1‑136 (MQ=255)
                                          gagGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTc                                                               <  5:147400/135‑1 (MQ=255)
                                          gagGACTTTCACCTCCAAGGCACCCACTTGGCCACCAAAACCACCGGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTc                                                               <  6:115836/135‑1 (MQ=21)
                                          gagGAATTTCACCTCCAAGTCACCCACTTGGCCACCACACCCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGAAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTc                                                               <  1:30643/135‑1 (MQ=255)
                                          gagCACTTTCACATCAACGTCACCCCATTGCCCACCACAACCACGCGTTTTGCGCTTACGCCCAACGCGCCATGCCTGGCGAACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCa                                                              <  4:279546/136‑1 (MQ=255)
                                           agGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCa                                                              <  7:59836/135‑1 (MQ=255)
                                            ggACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAg                                                             <  2:280053/135‑1 (MQ=255)
                                               cTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGCGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAAc                                                         >  1:49605/1‑136 (MQ=255)
                                                tttCCCCACCACGCCACCCAATTGCCAACCACACCCACGCGGGTTGCGTTAACGCCAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACg                                                        <  8:147463/136‑1 (MQ=21)
                                                tttCACCTCCAAGTCCCCCACTTGGCCACACAAACCACGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACg                                                        <  4:254976/136‑1 (MQ=21)
                                                tttCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCCATCCTGGCGCACACAAAAAAACGGCGCCTGCCAGAGCGCCCGGTTTTGCCTGAAGCCTGGTTGAGGaaag                                                        >  4:124231/1‑134 (MQ=17)
                                                tttCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACg                                                        >  6:87738/1‑136 (MQ=255)
                                                tttCACCTCCAAGTCACCCACTTGGCCACAACAACCACGCGGGTTGCGCTTACGCGAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACg                                                        <  6:73028/136‑1 (MQ=255)
                                                tttCACCACCACGGCACCAACTTGGCCACCACACCCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACg                                                        <  3:273213/136‑1 (MQ=21)
                                                tttAACCTCCACGTCACCAACTTGGCCACCACAACCACGCGGGTTGCGCTTACGCGAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACg                                                        <  8:95924/136‑1 (MQ=21)
                                                ttacACCTCAAAGCAACCCAATTGCCCACCACAACACAGCTGTGGGCTCTTACGCGAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACg                                                        <  4:55081/133‑1 (MQ=21)
                                                 tccACCTCAACGTCACCCACTTGGCAACCACAACAACGGGGTTTGCGTTAACGCGAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACg                                                        <  3:342292/133‑1 (MQ=21)
                                                  taaCCTCAAATTCACCCACTTGGCAACCACAACAAAGGGGGTTGCTCTTACGCGCAACGCGCCATGCCTGGCTCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACg                                                        <  1:305459/132‑1 (MQ=21)
                                                  tCACCTCCACGCCCCCCACTTGCCCCCCACACCCACGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACgg                                                       <  1:155003/135‑1 (MQ=25)
                                                  tCACCTCCAAGTCACCCACTTGGCCACCACAACCACGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACg                                                        <  3:49928/134‑1 (MQ=255)
                                                  tCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGTTTGCGGTTAGGGGAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTTCATCAAGCCTGCTTCAGGAACg                                                        <  5:134052/134‑1 (MQ=21)
                                                  tCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACg                                                        <  1:200726/134‑1 (MQ=255)
                                                  tCACCTCCAAGTCACCCACTTGCCAACCACAACCACGCGGGTTGCGCTTACGCGAAACGCGCCATGTCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACgg                                                       <  8:87015/135‑1 (MQ=25)
                                                  acACCTCCAAGTCAACCAGTTGGCCACCACAACCACGGGTGTTGCGTTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACg                                                        <  6:110006/133‑1 (MQ=21)
                                                   cACCTCCATGACACCCACTTGGCCACCACACCCACGCGTGTTGCGCTAACGCGAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGAt                                                     <  8:145032/136‑1 (MQ=25)
                                                   cACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCGTTCGCGCCACCCGCCAAGCCTGGCGCACACAAAAAAACGGCGCCAGCAAGAGCGCCCGGTTTTGGATAAAGCCAGCTTCAGGAACCGa                                                      >  3:137701/1‑135 (MQ=17)
                                                   cACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCGTACGCGCAACCCGCCCTGCCTGGCGCACACAAAAAAACGGCGCCATCAAGAGCGCCCGGTTTTTGCTAAAGCCTGCGTCACGTAAGGAt                                                     >  1:6060/1‑136 (MQ=14)
                                                    cccTCCAAGGCACCCCCTTGCCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGAACACAAAAAACGGGCTCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGAt                                                     <  6:93039/134‑1 (MQ=21)
                                                     ccTCCAAGTCACCCACTTGGCCACCACACCCAAGCGGGTGGGGATTAGGGGAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATaa                                                   <  1:11965/136‑1 (MQ=25)
                                                     ccTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATa                                                    >  7:250514/1‑135 (MQ=255)
                                                     ccTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTAAGCGCCACCCGCCATGCCTGGCGCACACAAAAAAAGGGCGCCTGCAAGCGCGCCCGTTTTTTGATGAAGCCTGCGTGAGGCACGGGTaa                                                   >  1:82779/1‑136 (MQ=17)
                                                        ccAAGTCACCCACTTGCCCACCACAACCAAGCGGGTTGCGCTAACGCGAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATcc                                                <  3:208548/136‑1 (MQ=255)
                                                          aaGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGt                                              <  8:249860/136‑1 (MQ=255)
                                                          aaGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGt                                              <  1:210636/136‑1 (MQ=255)
                                                          aaGTCACCCACTTGCCCCCCACAACCACGCGGGTTGCGCTTACGCCAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGt                                              <  5:117106/136‑1 (MQ=25)
                                                          aaGTCACCCACTTGCCCACCACACCCATGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGt                                              <  3:306685/136‑1 (MQ=255)
                                                          aaGACACCCACTGGGCCACCCCAACCACGCGGGTGGGCCTAACGCCCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGt                                              <  3:190468/136‑1 (MQ=25)
                                                            gTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACCCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTTCATCAAGCCGGCTTAAGGAACGGATAATCCGta                                             >  8:60097/1‑135 (MQ=255)
                                                            gTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCGTTCGCGCAACGCGCCATGCCTGGCGCACACAAAAAACCGGCGCCTGCCAGAGCGCCCGTTTTTGCCTCAAGCCTGCTTCATGAACCGATAAACCGta                                             >  1:48258/1‑135 (MQ=21)
                                                                  cACTTGGCCACCACAACCACGCGGGTTGCGCTAACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCtt                                      <  5:263285/136‑1 (MQ=255)
                                                                   aCTTGGCCACCACAACCAAGCGGGTTGCGCTTAAGCGCAACGCGCCATGCCTGGCGCCCACAAAAAAAGGGCCCCCGGAAGCGCCCCCGGTTTTGCATAAACCCAGCTTGAGGAAACGATTATTCGTATAaccgt                                      >  6:68297/1‑133 (MQ=9)
                                                                        gCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCTTCAGGc                                 <  2:259414/135‑1 (MQ=255)
                                                                        gCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCTTCAGGc                                 <  6:101598/135‑1 (MQ=255)
                                                                          caccacAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTTCATCAAGCCAGCTTCAGGAACGGATAATCCGTATACCCTTCAGGCCCa                              >  1:256613/1‑136 (MQ=255)
                                                                             cacaACCACGGCGGTTGCGAGTACGCGCAACGCGCCATGCCTGGCGCACACAAAAACCGGGCTCCTGCAAGAGCGCGCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCTTCAGGCCCATTg                           <  1:328519/136‑1 (MQ=37)
                                                                                aaCCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCTTCAGGCCCATTGGCg                        >  5:298402/1‑136 (MQ=255)
                                                                                aaCCAAGCGGGTTGCGCTTACGCGCAACCCGCCCTTCCTGGCGCCCACAAAAAACCGGCGCCGGCAAAAGCGCCCGTTTTTGGATCAAGCCTGGTTCAGGGACGGATAATCCGTTTACCCATGACGCCCATTTGCg                        >  7:36419/1‑136 (MQ=21)
                                                                                 aCCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCTTCAGGCCCATTGGCGt                       <  3:375511/136‑1 (MQ=255)
                                                                                        gggTTGCGCTTACGCGCAACCCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTTGATCAAGCCTGCTTCAGGAACGGATAATCCGTATAACCTTAAGGCCCATTGGCGGAATCGa                 >  1:304106/1‑135 (MQ=38)
                                                                                             gcgcTTACGCGAAACGCGCCATGCCTGCCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCTTCAGGCCCATTGGCGTAATCGAACTTCg           <  2:374948/136‑1 (MQ=255)
                                                                                              cgcTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCTTCAGGCCCATTGGCGTAATCGAACTTCg           <  1:117397/135‑1 (MQ=255)
                                                                                              cgcTTACGCGAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCTTCAGGCCCATTGGCGTAATCGAACTTCg           <  6:106092/135‑1 (MQ=255)
                                                                                              cgcTTACGCGAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCTTCAGGCCCATTGGCGTAATCGAACTTCg           <  5:72062/135‑1 (MQ=255)
                                                                                              cgcTTACGCCCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCTTCAGGCCCATTGGCGTAATCGAACTTCg           <  5:254206/135‑1 (MQ=255)
                                                                                                  tACGCGAAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCTTCAGGCCCATTGGCGTAATCGAACTTCGCCCgg      <  4:294631/136‑1 (MQ=255)
                                                                                                       gcAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCTTCAGGCCCATTGGCGTAATCGAACTTCGCCCGGTGCt  >  7:2488/1‑135 (MQ=255)
                                                                                                        |                                                                                                                                       
CACCCTTGCCTCCGGCTAACACTTCCCCTTGCCGGGTGTGTAGAGGACTTTCACCTCCAAGTCACCCACTTGGCCACCACAACCAAGCGGGTTGCGCTTACGCGCAACGCGCCATGCCTGGCGCACACAAAAAACGGGCGCCTGCAAGAGCGCCCGTTTTTGCATCAAGCCTGCTTCAGGAACGGATAATCCGTATACCCTTCAGGCCCATTGGCGTAATCGAACTTCGCCCGGTGCT  >  ACB143/3588784‑3589021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: