Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	1,652,260	C→T	intergenic (‑11/+12)	CJ019_01548 ← / ← nudC_1	hypothetical protein/NADH pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	1,652,260	0	C	T	100.0%	203.5 / NA	56	intergenic (‑11/+12)	CJ019_01548/nudC_1	hypothetical protein/NADH pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/38);  total (18/38)

GGCTTGCTGCGAAGGCGAGGGAGTTTGCGCTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATCCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGATAGTAGCATTCTAGTGTGCGAGTGACGGTGCGGAAAGCCAGCTCG  >  ACB143/1652127‑1652392                                                                                                                                      |                                                                                                                                     ggCTTGCTGCGAAGGCGAGGGAGTTTGCGCTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATtcc                                                                                                                                    >  4:50283/1‑136 (MQ=255)  gCTTGCTGCGAAGGCGAGGGAGTTTGCGCTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATtcc                                                                                                                                    <  4:137591/135‑1 (MQ=255)  gCTTGCTGCGAAGGCGAGGGAGTTTGCGCTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATtcc                                                                                                                                    <  4:132016/135‑1 (MQ=255)  gCTTGCTGCGAAGGCGAGGGAGTTTGCGCTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATtcc                                                                                                                                    <  8:354723/135‑1 (MQ=255)   cTTGCTGCTAAGGCGAGGGAGTTTGCGCTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTg                                                                                                                                  <  4:101615/136‑1 (MQ=255)   cTTGCTGCGAAGGCGAGGGAGTTTGCGCTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGTGCATGTAACAGTATTCCTg                                                                                                                                  <  4:261923/136‑1 (MQ=255)   cTTGCTGCGAAGGCGAGGGAGTTTGCGCTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTg                                                                                                                                  <  1:161355/136‑1 (MQ=255)   cTTGCTGCGAAGGCGAGGGAGTTTGCGCTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTg                                                                                                                                  <  5:39617/136‑1 (MQ=255)               gCGAGGGAGTTTGCGCTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAgg                                                                                                                      <  6:114805/136‑1 (MQ=255)               gCGAGGGAGTTTGCGCTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAgg                                                                                                                      <  1:371494/136‑1 (MQ=255)                cGAGGGAGTTTGCGCTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGgt                                                                                                                     >  6:131110/1‑136 (MQ=255)                 gAGGGAGTTTGCGCTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGgtg                                                                                                                    >  3:67298/1‑136 (MQ=255)                            cgcTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAAc                                                                                                         >  7:231650/1‑136 (MQ=255)                            cgcTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAAc                                                                                                         >  8:271098/1‑136 (MQ=255)                                   ggCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATc                                                                                                  >  7:6223/1‑136 (MQ=255)                                      gcgGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATcggc                                                                                               <  8:331089/136‑1 (MQ=255)                                         ggCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCggcggc                                                                                            <  6:296003/136‑1 (MQ=255)                                         ggCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCggcggc                                                                                            <  8:211363/136‑1 (MQ=255)                                                ggaggaTGGGATCGGGGAAGGCCGCCGGGGACACCGAGGTAACGCAAAGGCAGAACAGCGCAAGCTTCCTGCGCATGTATCAGTATTCCTGAAGGCGGGTTGGTGTGTGGCGAAAGTTTTATCGTCGGCAGGTAtt                                                                                     <  6:65478/136‑1 (MQ=255)                                                ggATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTAtt                                                                                     <  1:89344/136‑1 (MQ=255)                                                 gatgatGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTc                                                                                    <  8:239273/136‑1 (MQ=255)                                                   tgatgGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCAt                                                                                  <  3:16652/136‑1 (MQ=255)                                                   tgatgGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCAt                                                                                  <  1:259601/136‑1 (MQ=255)                                                      tgGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGc                                                                               <  4:67298/136‑1 (MQ=255)                                                       ggTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCTGGTATTCATGGcc                                                                              >  6:316802/1‑136 (MQ=255)                                                       ggTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGcc                                                                              >  7:41963/1‑136 (MQ=255)                                                       ggTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTAGTCATGGcc                                                                              >  2:129374/1‑136 (MQ=255)                                                            cGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGTCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATg                                                                          <  3:283803/135‑1 (MQ=255)                                                            cGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATg                                                                          <  3:308688/135‑1 (MQ=255)                                                            cGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATg                                                                          <  2:17949/135‑1 (MQ=255)                                                            cGGTGAAGACCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTAGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATg                                                                          <  1:186859/135‑1 (MQ=255)                                                              gTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATgggg                                                                       >  2:354604/1‑136 (MQ=255)                                                                           gggACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGa                                                          >  7:328465/1‑136 (MQ=255)                                                                             gACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATg                                                        <  7:62208/136‑1 (MQ=255)                                                                             gACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATg                                                        <  1:336655/136‑1 (MQ=255)                                                                              acacCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGc                                                       <  2:353246/136‑1 (MQ=255)                                                                                 ccGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGt                                                    <  8:54846/136‑1 (MQ=255)                                                                                   gAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTcg                                                  >  1:264927/1‑136 (MQ=255)                                                                                   gAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGCAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTcg                                                  <  7:271098/136‑1 (MQ=255)                                                                                     ggTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTg                                                                                                                                  <  4:255906/54‑1 (MQ=255)                                                                                     ggTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTg                                                                                                                                  >  3:255906/1‑54 (MQ=255)                                                                                     ggTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTcgcg                                                >  4:238070/1‑136 (MQ=255)                                                                                      gTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGa                                               <  8:328465/136‑1 (MQ=255)                                                                                      gTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGa                                               <  3:353539/136‑1 (MQ=255)                                                                                      gTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGa                                               <  3:326105/136‑1 (MQ=255)                                                                                      gTAACCCAAAGGCAGATAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGGGTGTGGCGAAACGTTCATCGGCGGCAGGGATTCATTGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGa                                               <  7:298822/136‑1 (MQ=255)                                                                                         aCGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGAta                                             <  2:16342/135‑1 (MQ=255)                                                                                           gCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGAtagta                                          <  3:242228/136‑1 (MQ=255)                                                                                           gCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGAtag                                            >  6:149866/1‑134 (MQ=255)                                                                                             aaaGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGAtagtag                                         >  2:316422/1‑135 (MQ=255)                                                                                                        aGGGCAAGCAACCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGATAGTAGCATTCTAGtgtg                             >  4:180832/1‑136 (MQ=255)                                                                                                              agcaaCCAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCATGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGTTATGAGCATTATAGGGTGCGAGtt                       >  3:14843/1‑135 (MQ=255)                                                                                                                   ccAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGATAGTAGCATTCTAGTGTGCGAGTGACGGt                  <  4:261516/136‑1 (MQ=255)                                                                                                                   ccAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGATAGTAGCATTCTAGTGTGCGAGTGACGGt                  <  6:5145/136‑1 (MQ=255)                                                                                                                   ccAGCGCATGTAACAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGATAGTAGCATTCTAGTGTGCGAGTGACGGt                  <  6:213643/136‑1 (MQ=255)                                                                                                                               aCAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGATAGTAGCATTCTAGTGTGCGAGTGACGGTGCGGAAAGCCa       <  8:6223/135‑1 (MQ=255)                                                                                                                               aCAGTATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGATAGTAGCATTCTAGTGTGCGAGTGACGGTGCGGAAAGCCAg      >  2:292502/1‑136 (MQ=255)                                                                                                                                  gTATTCCTGAAGGCGGGTAGGTGTGTGGCGACACGTTGATCGGCGGCAGGTATTCTTGGCCTAGGGGGTAGTGCTGGCCGATGCGGTCGCGATAGTAGCATTCTAGTGTGCGAGTGACGGTGCGGAAAGCCAGCTc   <  8:236756/136‑1 (MQ=255)                                                                                                                                   tATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGATAGTAGCATTCTAGTGTGCGAGTGACGGTGCGGAAAGCCAGCTCg  <  2:264927/136‑1 (MQ=255)                                                                                                                                   tATTCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGATAGTAGCATTCTAGTGTGCGAGTGACGGTGCGGAAAGCCAGCTCg  <  2:260225/136‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     GGCTTGCTGCGAAGGCGAGGGAGTTTGCGCTGCCGGCGCGGGCTTGCGGATGATGGTTTCGGTGAAGGCCGCCTGGGACACCGAGGTAACGCAAAGGCAGAAAAGGGCAAGCAACCAGCGCATGTAACAGTATCCCTGAAGGCGTTTAGGTGTGTGGCGAAACGTTCATCGGCGGCAGGTATTCATGGCCTATGGGGTAGTGCTGGCCGATGCGGTCGCGATAGTAGCATTCTAGTGTGCGAGTGACGGTGCGGAAAGCCAGCTCG  >  ACB143/1652127‑1652392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑