Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	1,735,045	T→C	intergenic (‑77/+7)	CJ019_01633 ← / ← CJ019_01634	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	1,735,045	0	T	C	100.0%	186.3 / NA	56	intergenic (‑77/+7)	CJ019_01633/CJ019_01634	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/19);  total (37/19)

GGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGCAGCCGAGCGGGTCAGGCA  >  ACB143/1734920‑1735176                                                                                                                              |                                                                                                                                    ggACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGACTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGtg                                                                                                                             >  4:321593/1‑133 (MQ=255)          gCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGACGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCa                                                                                                                  >  1:233936/1‑136 (MQ=255)           cccGCTGATCAGGATCGGGCGTTCCTTTGCTTGATACATATCTTCGCCTTTGCAGCCATATATCGGCAGTGAATAGAGTGGATAGGGGTTACAGCGGTGTGGGGTACAGATGTGCCCTGGGGTTAAGAGATGGCAc                                                                                                                 >  8:349048/1‑136 (MQ=255)              gCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGGGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCgg                                                                                                              <  7:261321/136‑1 (MQ=255)                   aCAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGt                                                                                                         <  3:339698/136‑1 (MQ=255)                        aTCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATAGCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGGGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCa                                                                                                    <  8:57483/136‑1 (MQ=255)                              cGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGtt                                                                                              >  3:308735/1‑136 (MQ=255)                               gTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTg                                                                                             >  1:243963/1‑136 (MQ=255)                               gTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTg                                                                                             >  1:289685/1‑136 (MQ=255)                                    tttGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGc                                                                                        >  5:64437/1‑136 (MQ=255)                                           gagaCATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGgc                                                                                  <  8:66682/135‑1 (MQ=255)                                            agaCATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGgcgc                                                                                <  3:175355/136‑1 (MQ=255)                                             gaCATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGcgcg                                                                               <  2:243963/136‑1 (MQ=255)                                               cttatcTTCGCCTTTGCCGCCATTTCGCGGCAGTTATTAGAGTGGATAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGTGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGc                                                                             <  5:322071/131‑1 (MQ=255)                                               cATAGCTTCGCCTTTGCCGCCATATCGCGTCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGc                                                                             <  4:259871/136‑1 (MQ=255)                                                aTAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCCCCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCa                                                                            >  2:323321/1‑136 (MQ=255)                                                     ttCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGAACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCActctc                                                                       >  3:63107/1‑135 (MQ=255)                                                     ttCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGAGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTa                                                                       >  8:73926/1‑136 (MQ=255)                                                          cTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTgg                                                                  >  5:116018/1‑136 (MQ=255)                                                              gcctccATATCGCGGCAGTGAATAGAGTGGAGAGGGGGTACATCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGc                                                               <  8:167156/135‑1 (MQ=255)                                                                 gccATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTc                                                           >  8:155672/1‑136 (MQ=255)                                                                 gccATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTc                                                           >  4:29947/1‑136 (MQ=255)                                                                 gccATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTc                                                           >  2:169451/1‑136 (MQ=255)                                                                                   aTAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCAtt                                         >  1:164612/1‑136 (MQ=255)                                                                                    tAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTc                                        <  2:338992/136‑1 (MQ=255)                                                                                    tAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCACAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTc                                        <  8:327345/136‑1 (MQ=255)                                                                                     agagTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTc                                        <  8:163103/135‑1 (MQ=255)                                                                                     agagTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTc                                        >  7:163103/1‑135 (MQ=255)                                                                                      gagTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCgg                                      <  1:370493/136‑1 (MQ=255)                                                                                            agagGGGTTACAGCGGGGTGGGGTATAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCAtgtg                                >  6:340193/1‑136 (MQ=255)                                                                                             gagGGGTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCAGCATTCGGCAtgtg                                >  6:164217/1‑135 (MQ=255)                                                                                               ggggTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCTGCATGTGTa                              >  2:355803/1‑135 (MQ=255)                                                                                               ggggTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTa                              >  8:32309/1‑135 (MQ=255)                                                                                                 ggTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTAccg                           >  6:183200/1‑134 (MQ=255)                                                                                                 ggTTACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTATACAg                           >  3:65874/1‑136 (MQ=255)                                                                                                    tACAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCg                        >  2:330650/1‑136 (MQ=255)                                                                                                      cAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGtt                      >  3:349820/1‑136 (MQ=255)                                                                                                      cAGCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGtt                      >  3:344273/1‑136 (MQ=255)                                                                                                        gCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCTCTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTg                     >  3:261774/1‑135 (MQ=255)                                                                                                        gCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGc                    >  1:168386/1‑136 (MQ=255)                                                                                                        gCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCAGTCGGCATGTGTACAGTCGTTg                     >  3:24411/1‑135 (MQ=255)                                                                                                        gCGGGGTGGGGTACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCAATCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTg                     >  3:187713/1‑135 (MQ=255)                                                                                                           gggtggggtACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGCAGc                 <  6:131492/136‑1 (MQ=255)                                                                                                           gggtggggtACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGCAGc                 <  3:14010/136‑1 (MQ=255)                                                                                                           gggtggggtACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGCAGc                 <  5:88649/136‑1 (MQ=255)                                                                                                             gtggggtACAGATGTGCCCGGGGGGTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGCAGCCg               <  7:100735/136‑1 (MQ=255)                                                                                                              tggggtACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGCAGcc                >  5:207216/1‑134 (MQ=255)                                                                                                              tggggtACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGCAGcc                >  8:180572/1‑134 (MQ=255)                                                                                                              tggggtACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGCAGcc                >  6:169155/1‑134 (MQ=255)                                                                                                               ggggtACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGCAGCCGAg             >  4:367372/1‑136 (MQ=255)                                                                                                                   tACAGATGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGCAGCCGAGCggg         >  8:156464/1‑136 (MQ=255)                                                                                                                        aTGTGCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGCAGCCGCGCGGGTCAgg    >  1:158673/1‑136 (MQ=255)                                                                                                                         tgtgCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGGCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGCAGCCGAGCGGGTCAGGc   <  2:243389/136‑1 (MQ=255)                                                                                                                           tgCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCaa                                                                                                   <  7:251043/38‑1 (MQ=255)                                                                                                                           tgCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCaa                                                                                                   >  8:251043/1‑38 (MQ=255)                                                                                                                           tgCCCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGAAGCCGAGCGGGTCAGGCa  >  8:332313/1‑135 (MQ=255)                                                                                                                              |                                                                                                                                    GGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTTCCGCATTCGGCATGTGTACAGTCGTTGCAGCCGAGCGGGTCAGGCA  >  ACB143/1734920‑1735176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑