Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 2,240,159 A→G F827S (TTT→TCT)  CJ019_02129 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,240,1590AG97.9% 175.0 / ‑4.3 48F827S (TTT→TCT) CJ019_02129hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (15/32);  total (15/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

CGACGATGCGGTTGTCGCGGGCATCCACCTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAAACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGGTGTGGTGCCGTGGTCGGGGATCAGCGCCACGCCGATGCTGGGCGTTACCTGCAGGC  >  ACB143/2240028‑2240291
                                                                                                                                   |                                                                                                                                    
cgacgaTGCGGTTGTCGCGGGCATCCACCTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAg                                                                                                                                  >  5:150434/1‑136 (MQ=255)
cgacgaTGCGGTTGTCGCGGGCATCCACCAGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGCCGCAGGTCGTTTTCCATGAGCAGGCCCTCGCTGGCGGCCTTCAGCATGGTGGTGTGAGACAg                                                                                                                                  >  3:89373/1‑136 (MQ=255)
     aTGCGGTTGTCGCGGGCATCCACCTGCGGCTGGAAGTGCAGTGCCAGCTCACCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTggg                                                                                                                             >  6:15541/1‑136 (MQ=255)
          gtggTCGCGGGCATCCACCTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGgtggtg                                                                                                                        <  4:119437/133‑1 (MQ=255)
                gcgGGCATCCACCTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGa                                                                                                                  <  7:2565/136‑1 (MQ=255)
                gcgGGCATCCACCTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGa                                                                                                                  >  5:342635/1‑136 (MQ=255)
                 cgGGCATCCACCTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGAc                                                                                                                 <  1:61035/136‑1 (MQ=255)
                 cgGGCATCCACCTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGAc                                                                                                                 <  3:68013/136‑1 (MQ=255)
                       tCCACCTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGt                                                                                                           <  8:282601/136‑1 (MQ=255)
                       tCCACCTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGt                                                                                                           <  8:258585/136‑1 (MQ=255)
                       tCCACCTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGt                                                                                                           <  2:348532/136‑1 (MQ=255)
                       tCCACCTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGt                                                                                                           <  5:62332/136‑1 (MQ=255)
                       tCCACCTGCGGCTGGAAGTGCAGGGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGt                                                                                                           <  5:3235/136‑1 (MQ=255)
                            cTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTg                                                                                                      <  8:101879/136‑1 (MQ=255)
                            cTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTg                                                                                                      <  1:79733/136‑1 (MQ=255)
                            cTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTg                                                                                                      <  1:34427/136‑1 (MQ=255)
                            cTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTg                                                                                                      <  8:290455/136‑1 (MQ=255)
                            cTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTg                                                                                                      <  8:305792/136‑1 (MQ=255)
                            cTGAGGCTGGAAGTGCAGTGCCAGCGCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTg                                                                                                      <  4:354697/136‑1 (MQ=255)
                                ggCTGGAAGTGCAGTGCCAGCGCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGccc                                                                                                  <  2:293681/136‑1 (MQ=255)
                                 gCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCg                                                                                                 <  5:271489/136‑1 (MQ=255)
                                    ggAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTa                                                                                              >  7:8834/1‑136 (MQ=255)
                                       agtgcagtgcCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAg                                                                                           <  1:83561/136‑1 (MQ=255)
                                               gcCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAAACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGatat                                                                                   <  6:325632/136‑1 (MQ=255)
                                                        ccACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGtt                                                                          <  3:7660/136‑1 (MQ=255)
                                                        ccACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGtt                                                                          <  6:367162/136‑1 (MQ=255)
                                                         cACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGttt                                                                         <  4:281356/136‑1 (MQ=255)
                                                          aCGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTg                                                                        <  5:43278/136‑1 (MQ=255)
                                                           cGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGttt                                                                         >  5:208020/1‑134 (MQ=255)
                                                               ccagggcctggCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGAGCGGAGTCCTTGGCCCGGTACAGGGCGATATTGGCACGTTTGAGctg                                                                   <  4:48265/136‑3 (MQ=255)
                                                                 agggccaggCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGt                                                                 >  1:129021/1‑136 (MQ=255)
                                                                           gcAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCgg                                                              >  3:105964/1‑129 (MQ=255)
                                                                           gcAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCgg                                                              <  4:105964/129‑1 (MQ=255)
                                                                           gcAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGgtgt                                                       >  5:125762/1‑136 (MQ=255)
                                                                           gcAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGgtgt                                                       >  4:271923/1‑136 (MQ=255)
                                                                           gcAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGgtgt                                                       >  1:315701/1‑136 (MQ=255)
                                                                                      tCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGGTGTGGTGCCGTGGt                                            >  3:13268/1‑136 (MQ=255)
                                                                                      tCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGGTGTGGTGCCGTGGt                                            >  3:64718/1‑136 (MQ=255)
                                                                                      tCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGGGGTGTTGCGACCGGAGCCCTTGGCCCGGTACAGGGCGATATTGGCACGTTTGAGCAGGTCGGCCGGTGTGGTGCCGTgg                                             <  1:322061/135‑1 (MQ=255)
                                                                                          tGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGGTGTGGTGCCGTGGTCggg                                        <  2:83838/136‑1 (MQ=255)
                                                                                                    ctcgctGGCGGCCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGGTGTGGTGCCGTGGTCGGGGATCAgcgc                               >  7:1385/1‑135 (MQ=255)
                                                                                                           gcggcCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGGTGTGGTGCCGTGGGCGGGGATCAGCGCCACGCCGa                       <  6:75506/136‑1 (MQ=255)
                                                                                                           gcggcCTTCTGCATGGTGGTGTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGGTGTGGTGCCGTGGGCGGGGATCAGCGCCACGCCGa                       <  2:13924/136‑1 (MQ=255)
                                                                                                           gcggcCTTCTGCATGGTGGTGTGAGACAGCGGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGGTGTGGTGCCGTGGTCGGGGATCAGCGCCACGCCGa                       <  2:20097/136‑1 (MQ=255)
                                                                                                                          gtggtgTGAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGGTGTGGTGCCGTGGTCGGGGATCAGCGCCACGCCGATGCTGGGCGTTAcc         <  3:14151/135‑1 (MQ=255)
                                                                                                                              tgtgAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGGTGTGGTGCCGTGGTCGGGGATCAGCGCCACGCCGATGCTGGGCGTTACCTGCAg    >  8:125392/1‑136 (MQ=255)
                                                                                                                                tgAGACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGGTGTGGTGCCGTGGTCGGGGATCAGCGCCACGCCGATGCTGGGCGTTACCTGCAGgc  <  6:366293/136‑1 (MQ=255)
                                                                                                                                tgAGACAGCTGGGGGGTGTTGCGACCGGAGTACTGGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGGTGTGGTGCCGTGGTCGGGGATCAGCGCCACGCCGATGCTGGGCGTTACCTGCAgg   <  4:220423/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                    
CGACGATGCGGTTGTCGCGGGCATCCACCTGCGGCTGGAAGTGCAGTGCCAGCTCGCCACGGGCCAGGGCCAGGCGCAGGTCGTTTTCCATGCGCAGGCGCTCGCTGGCGGCCTTCTGCATGGTGGTGTGAAACAGCTGGGTGGTGTTGCGACCGGAGTCCTTGGCCCGGTACAGGGCGATATCGGCACGTTTGAGCAGGTCGGCCGGTGTGGTGCCGTGGTCGGGGATCAGCGCCACGCCGATGCTGGGCGTTACCTGCAGGC  >  ACB143/2240028‑2240291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑