Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,727,032	C→T	R176R (AGG→AGA)	pip ←	Proline iminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,727,032	0	C	T	100.0%	124.9 / NA	43	R176R (AGG→AGA)	pip	Proline iminopeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/28);  total (15/28)

GGTTCGGAAAAGCGGTCGACCACCAGCGGGTTGGGGCGCAGGGTGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCCCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGCGGCCGGCACAGGAAGATGCCGC  >  ACB143/5726901‑5727162                                                                                                                                    |                                                                                                                                   ggTTCGGAAAAGCGGTCGACCACCAGCGGGTTGGGGCGCAGGGTGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCAACTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGa                                                                                                                                >  8:264487/1‑136 (MQ=255)      ggAAAAGCGGGCGACCACCAGCGGGTTGTGGCGGAGGGTGGCGGTACGGCCTTCCCAGGTGGAGCACGCCTTGGCTGAGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAgg                                                                                                                           <  2:83703/136‑1 (MQ=255)           aGCGGTCGACCACCAGCGGGTTGGGGCGCAGGGTGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTcgcc                                                                                                                      <  7:166010/136‑1 (MQ=255)                tCGACCACCAGCGGGTTGGGGCGCAGGGTGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGtt                                                                                                                 >  2:124159/1‑136 (MQ=255)                               ttGGGGCGCAGGGTGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCGTCCGGTGGAATCg                                                                                                  <  1:223693/136‑1 (MQ=255)                               ttGGGGCGCAGGGTGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCg                                                                                                  <  5:178057/136‑1 (MQ=255)                               ttGGGGCGCAGGGTGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCg                                                                                                  <  1:309022/136‑1 (MQ=255)                                        aGGGTGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGt                                                                                         <  2:175519/136‑1 (MQ=255)                                         gggTGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATg                                                                                          >  8:324852/1‑134 (MQ=255)                                          ggTGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAg                                                                                       >  5:318507/1‑136 (MQ=255)                                          ggTGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAg                                                                                       >  6:182897/1‑136 (MQ=255)                                           gTGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGt                                                                                      >  6:368315/1‑136 (MQ=255)                                           gTGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCGTGTGGAAGGCTCTGACCAGGTAGCCGCGTTCCTCCGGTGGAATAGGTGCGATGTAg                                                                                       >  4:116416/1‑135 (MQ=255)                                            tGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGAAGTAGTc                                                                                     >  2:314198/1‑136 (MQ=255)                                              gCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCt                                                                                   <  7:5680/136‑1 (MQ=255)                                                 gTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTTCCGGTCAGGCGCTTGTGGAAGGCTCTGCCCAGGTCGCCGCGTTCCTCCGGGGGCATAGGTGGGATGTAGTCCTGc                                                                                 >  4:220299/1‑135 (MQ=255)                                                                   gACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAggc                                                              <  7:270127/136‑1 (MQ=255)                                                                   gACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAggc                                                              <  7:18336/136‑1 (MQ=255)                                                                                        aTCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAg                                          >  6:31328/1‑135 (MQ=255)                                                                                           tGGGCGATCTGGTCGTGACCGGTCATGCGCTTGTGGAACGCTCTGTCCAGGTCGCCGCGTTCCTCCGGGGGATTCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAAGAGGCGGCTGGCGCCTTCCTGGTAGatc                                       <  4:36445/135‑3 (MQ=255)                                                                                             ggtgATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAAc                                       <  6:35613/130‑1 (MQ=255)                                                                                             ggCGATCTGGGCGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAAc                                       <  2:379376/133‑1 (MQ=255)                                                                                                     ggTCGTTAACGGTCACGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGATACTGCGGTGGAATCGGTGCCATGAAGTCCTGCCAGTAGTCGGGGAACATGCGGCTGGCGCCATCCAGGGAGTACCACTCGAttta                            >  4:67016/1‑135 (MQ=255)                                                                                                      ggcGTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGAt                               <  3:109683/130‑1 (MQ=255)                                                                                                         gTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGc                        <  8:100684/136‑1 (MQ=255)                                                                                                         gTTACCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGc                        <  1:219538/136‑1 (MQ=255)                                                                                                            aCCGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGcggc                     <  5:251852/136‑1 (MQ=255)                                                                                                              cGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGcggccg                   <  2:16677/136‑1 (MQ=255)                                                                                                              cGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAAACGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGcggccg                   <  5:75055/136‑1 (MQ=255)                                                                                                              cGGTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGGTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGcggccg                   <  7:104475/136‑1 (MQ=255)                                                                                                               ggTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGcggccgg                  >  4:88471/1‑136 (MQ=255)                                                                                                               ggTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGcggccgg                  >  7:159637/1‑136 (MQ=255)                                                                                                               ggTCAGGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGcggccgg                  >  4:183029/1‑136 (MQ=255)                                                                                                               cgTCACGCGCTTGTGGAAGGCTCTGACCAGGTCGCCGCGTTCCACCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCGGGCGCCATCCTGGTAGAACCACTCGATTTCCTGcggccgg                  >  4:188494/2‑136 (MQ=255)                                                                                                                           gtgGAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTATAACCACTCGATTTCCTGCGGACGGCACAGGAAGa        <  1:284276/134‑1 (MQ=255)                                                                                                                             ggAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGCGGCCGGCACAGGAAGATGcc    <  1:307512/136‑1 (MQ=255)                                                                                                                             ggAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCAGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGCGGCCGGCACAGGAAGATGcc    <  8:267167/136‑1 (MQ=255)                                                                                                                              gAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCTATTTCCTGCGGCCGGCACAGGAAGATGcc    <  2:243171/135‑1 (MQ=255)                                                                                                                              gAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGCGGCCGGCACAGGAAGATGcc    <  3:297108/135‑1 (MQ=255)                                                                                                                              gAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGCGGCCGGCACAGGAAGATGcc    <  4:311684/135‑1 (MQ=255)                                                                                                                              gAAGGCTCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGCGGCCGGCACAGGAAGATGcc    <  5:155158/135‑1 (MQ=255)                                                                                                                              gAAGGCTCTGACCAGGGCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGCGGCCGGCACAGGAAGATGcc    <  1:304314/135‑1 (MQ=255)                                                                                                                               caGGCTCTGACCAGGGCGCCGCGGTCCACCGGTGGAATCGGTGCGATGTACTCCAGCCAGAAGTCGGGCAACCGCCGGCTGGCGCCTTCCAGGACGAACCACTCGATTTCCTGCGGCCGGCACAGGAAGATGCcgc  <  2:38944/135‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   GGTTCGGAAAAGCGGTCGACCACCAGCGGGTTGGGGCGCAGGGTGGCGGTACGGCCTTCCCAGGTGGACCACGCCTTGGCGGCGTGCATCTGGGCGATCTGGTCGTTACCGGTCAGGCGCTTGTGGAAGGCCCTGACCAGGTCGCCGCGTTCCTCCGGTGGAATCGGTGCGATGTAGTCCTGCCAGTAGTCGGGGAACAGGCGGCTGGCGCCTTCCTGGTAGAACCACTCGATTTCCTGCGGCCGGCACAGGAAGATGCCGC  >  ACB143/5726901‑5727162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑