Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	757,847	T→C	Y181C (TAC→TGC)	fliY_2 ←	L‑cystine‑binding protein FliY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	757,847	0	T	C	100.0%	205.4 / NA	59	Y181C (TAC→TGC)	fliY_2	L‑cystine‑binding protein FliY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/22);  total (37/22)

GGTGCGCCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGTAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGAACCCACCTTGCGGCCTT  >  ACB143/757715‑757973                                                                                                                                     |                                                                                                                               ggTGCGCCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAgg                                                                                                                             <  6:212788/136‑1 (MQ=255) ggTGCGCCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAgg                                                                                                                             >  2:30824/1‑136 (MQ=255)    gcgcCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGgcg                                                                                                                          >  8:86756/1‑136 (MQ=255)    gcgcCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGgcg                                                                                                                          >  5:24843/1‑136 (MQ=255)    gcgcCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGgcg                                                                                                                          >  2:158609/1‑136 (MQ=255)    gcgcCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGgcg                                                                                                                          >  6:32264/1‑136 (MQ=255)    gcgcCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGgcg                                                                                                                          >  4:351600/1‑136 (MQ=255)      gcCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGgcg                                                                                                                          >  6:188840/1‑134 (MQ=255)      gcCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGgcg                                                                                                                          >  1:268093/1‑134 (MQ=255)      gcCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGgcg                                                                                                                          >  8:180436/1‑134 (MQ=255)      gcCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGgcg                                                                                                                          >  7:151337/1‑134 (MQ=255)        cGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGc                                                                                                                      >  7:313009/1‑136 (MQ=255)                  gAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCCCGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCCGTGTCGCAGGGCGCAGCCTGCACGAAg                                                                                                            >  2:105027/1‑136 (MQ=255)                                 ctgcAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAAACg                                                                                             <  3:213792/136‑1 (MQ=255)                                    cAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGAtc                                                                                          >  2:254450/1‑136 (MQ=255)                                    cAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGAtc                                                                                          >  2:236457/1‑136 (MQ=255)                                       gcgcGGCTGCGCGGTCA‑CAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGAtcttct                                                                                      <  4:140824/136‑1 (MQ=255)                                        cgcgGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGAtcttct                                                                                      <  6:204283/136‑1 (MQ=255)                                                   ggTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTg                                                                           >  2:239157/1‑136 (MQ=255)                                                           ggTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGGCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGGAGCCTTGACGTAGTCGAACGGGTATTCTATCTTCTGGTCTTCCTTGCCTTCAAt                                                                   <  2:29092/136‑1 (MQ=255)                                                                gTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCa                                                              <  7:180436/136‑1 (MQ=255)                                                                 tAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAg                                                             <  6:24843/136‑1 (MQ=255)                                                                  aaTGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAgg                                                            >  1:146353/1‑136 (MQ=255)                                                                  aaTGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCGGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAgg                                                            >  5:228380/1‑136 (MQ=255)                                                                       tgGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCtt                                                       <  1:306184/136‑1 (MQ=255)                                                                         gCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTgg                                                     >  3:52362/1‑135 (MQ=255)                                                                         gCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGt                                                     >  3:93550/1‑136 (MQ=255)                                                                         gCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGt                                                     >  3:113750/1‑136 (MQ=255)                                                                         gCGTCCAGGCGCACGCCAGTGCCCAGCCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAACTCGAACGGGGAATCGATCTTCTGGTCTTCCTTTCCTTCAATGACCAGGTTCTTGt                                                     >  6:358177/1‑136 (MQ=255)                                                                             ccAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCGTCCTTGCCTTCAATGACCAGGTTCTTGTTCAg                                                 >  3:372484/1‑136 (MQ=255)                                                                              cAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAgg                                                <  2:297084/136‑1 (MQ=255)                                                                              cAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAgg                                                >  5:28865/1‑136 (MQ=255)                                                                                      cGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTc                                        <  8:151337/136‑1 (MQ=255)                                                                                      cGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTc                                        <  1:246452/136‑1 (MQ=255)                                                                                      cGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTc                                        <  2:364146/136‑1 (MQ=255)                                                                                      cGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGGTTTCAGTGTCGCAGGGCGCAGCCGGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTc                                        <  1:251640/136‑1 (MQ=255)                                                                                               ccAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGACTTCAATGACCAGGTGCTTGTTCAGGTAGCTTTCGTAGCTCGa                               >  2:87428/1‑136 (MQ=255)                                                                                                 aGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATg                             <  2:229035/136‑1 (MQ=255)                                                                                                    cccAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTTGCTCGATGCAc                          >  4:136663/1‑136 (MQ=255)                                                                                                    cccAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCAc                          >  7:53878/1‑136 (MQ=255)                                                                                                    cccAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCAc                          >  7:115084/1‑136 (MQ=255)                                                                                                      cAAGTCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACt                         >  8:166413/1‑135 (MQ=255)                                                                                                          tCCTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCg                    <  4:96849/136‑1 (MQ=255)                                                                                                           ccTGGAAGGCGACGTTTTCAGTTTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGTTTCGTGTTAAGGGAGATTTCGTAGCTCGATGCACTGATCg                    >  8:303554/1‑135 (MQ=255)                                                                                                           ccTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCg                    >  6:292856/1‑135 (MQ=255)                                                                                                            cTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGa                   <  6:164244/135‑1 (MQ=255)                                                                                                            cTGGAAGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGa                   <  8:244576/135‑1 (MQ=255)                                                                                                                aaGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGAACCCa              >  7:17544/1‑136 (MQ=255)                                                                                                                aaGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGAACCCa              >  3:186482/1‑136 (MQ=255)                                                                                                                 aGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGAACCCAc             >  7:186722/1‑136 (MQ=255)                                                                                                                 aGGCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGAACCCAc             >  3:127852/1‑136 (MQ=255)                                                                                                                   gCGACGTTTTCCTGGTCGCAGGGCGCCGCCTGCACGAAGGCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGAACCCACCt           <  8:239058/136‑1 (MQ=255)                                                                                                                   gCGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGAACCCACCt           <  6:198780/136‑1 (MQ=255)                                                                                                                    cGACGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGAACCCACCtt          <  7:307116/136‑1 (MQ=255)                                                                                                                       cGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGAACCCACCTTGCg       >  3:107906/1‑136 (MQ=255)                                                                                                                       cGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGAACCCACCTTGCg       >  3:127332/1‑136 (MQ=255)                                                                                                                       cGTTTTCAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGAACCCACCTTGCg       >  5:69131/1‑136 (MQ=255)                                                                                                                             cAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGAACCCACCTTGCGGCCtt  <  2:274840/135‑1 (MQ=255)                                                                                                                             cAGTGTCGCAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGAACCCACCTTGCGGCCtt  <  5:279089/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                               GGTGCGCCGGCCAGCTTGAAGCGCTTGTCTTGCTGCAGGCGCGGCTGCGCGGTCACCAGGTTGGTAATGATGGCGTCCAGGCGCACGCCAGTGCCCAGGCCCAAGTCCTGGAAGGCGACGTTTTCAGTGTCGTAGGGCGCAGCCTGCACGAAGTCGAACGGGTAATCGATCTTCTGGTCTTCCTTGCCTTCAATGACCAGGTTCTTGTTCAGGTAGCTTTCGTAGCTCGATGCACTGATCGAACCCACCTTGCGGCCTT  >  ACB143/757715‑757973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑