| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| JC | ACB143 | 5,390,260 | +GTGCCGATGGA | 33.3% | coding (354/1666 nt) | ligH → | 10‑formyltetrahydrofolate synthetase |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | ACB143 | = 5390260 | 33 (0.620) | 16 (0.300) +GTGCCGATGGA |
9/194 | NT | 33.3% | coding (354/1666 nt) | ligH | 10‑formyltetrahydrofolate synthetase |
| ? | ACB143 | 5390261 = | 34 (0.570) | coding (355/1666 nt) | ligH | 10‑formyltetrahydrofolate synthetase | |||||
ACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > ACB143/5390126‑5390260‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCC > ACB143/5390261‑5390390 ||||||||||| ACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCG < 6:367104/136‑1 GACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCG < 1:32442/136‑1 GACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCG < 1:53306/136‑1 CCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGAT < 8:294202/136‑1 CCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGAT < 7:51714/136‑1 CCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGAT > 3:67015/1‑136 CACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATG < 1:244961/136‑1 CACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATG < 2:165693/136‑1 CACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCCTGCCGATG > 4:9011/1‑136 GACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGTGCCGATGGAGGA > 6:394043/1‑136 GGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGTGCCGATGGAGGACATCAACCTTCACTTCAC > 1:207814/1‑136 GCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGTGCCGATGGAGGACATCAACCTTCACTTCACC < 5:394043/136‑1 AGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGAC > 7:21091/1‑136 GCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGC > 4:275378/1‑136 CGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCG < 5:204103/136‑1 GTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGTGCCGATGGAGGACATCCACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGA > 4:124203/1‑136 TGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGAC < 1:265349/136‑1 GCAGGTCGTGCCGATGGAGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCC > 6:334404/1‑136 GATGGAGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCC < 3:397626/136‑1 ||||||||||| ACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > ACB143/5390126‑5390260‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCC > ACB143/5390261‑5390390 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |