Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,390,652 T→G 12.1% L249R (CTG→CGG)  ligH → 10‑formyltetrahydrofolate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,390,6520TG12.1% 127.7 / 9.1 47L249R (CTG→CGG) ligH10‑formyltetrahydrofolate synthetase
Reads supporting (aligned to +/- strand):  ref base T (15/26);  new base G (2/4);  total (17/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCGATCCTGTGCCTTTCCTCGGACCTCAAGGACCTCGAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGC  >  ACB143/5390520‑5390787
                                                                                                                                    |                                                                                                                                       
tGGCGATCCTGTGCCTTTCCTCGGACCTCAAGGACCTCGAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTgctgct                                                                                                                                      >  6:106638/1‑136 (MQ=255)
   cGATCCTGTGCCTTTCCTCGGACCTCAAGGACCTCGAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCaa                                                                                                                                   <  5:181407/136‑1 (MQ=255)
             ccTTTCCTCGGCCCTCAAGGACCTCCAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCTTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTg                                                                                                                         <  4:296278/136‑1 (MQ=255)
             ccTTTCCTCGGACCTCAAGGACCTCGAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTg                                                                                                                         <  6:115829/136‑1 (MQ=255)
              cTTTCCTCGGACCTCAAGGACCTCGAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTg                                                                                                                         >  4:352630/1‑135 (MQ=255)
                ttCCTCGGACCTCTAGGACCTCGTGCGGCGCCGGGGAAAATAGCACGCCGGCTACACGCGGGAGGGGAAGGCCGTGCTGGCCAGCGATCTGAATGCCTCGTGCGACATGACCGTGGTGCTCAAGGTCGCGCTGCa                                                                                                                       <  4:387828/135‑1 (MQ=255)
                    tCGGACCTCAAGGACCTCGAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCg                                                                                                                   >  5:67215/1‑135 (MQ=255)
                      ggccgtCACGGACCTCCAGCGGCGCCTCGGCAACCTGCACTCCGGCTACCCGCTCGAGCGAAAGGCCGTGCTGGCCAGCGAGAGGAATGCCTCGGGCGCCATGACCGGGCGGCTCAAGGACGCGCTGCAGCCGa                                                                                                                  <  8:80313/129‑1 (MQ=255)
                      ggACCTCAAGGACCTCGAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGa                                                                                                                  <  2:330469/134‑1 (MQ=255)
                      ggACCTCAAGGACCTCGAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGa                                                                                                                  <  1:211845/134‑1 (MQ=255)
                      gacccgcAAGCACCGCGAGCGGCGCCCCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGa                                                                                                                  <  2:354246/128‑1 (MQ=255)
                               ggACCTCGAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGtgct                                                                                                                                         >  4:260566/1‑102 (MQ=255)
                               ggACCTCGAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGtgct                                                                                                                                         <  3:260566/102‑1 (MQ=255)
                                  ccTCGAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACg                                                                                                    <  5:370290/136‑1 (MQ=255)
                                  ccTCGAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACg                                                                                                    <  3:331578/136‑1 (MQ=255)
                                  ccTCGAGCGCCGCCTCGGCCACCTCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACg                                                                                                    <  2:181059/136‑1 (MQ=255)
                                      gAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCt                                                                                                  <  2:248630/134‑1 (MQ=255)
                                       aGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGga                                                                                               <  7:91661/136‑1 (MQ=255)
                                       aGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGga                                                                                               <  7:17597/136‑1 (MQ=255)
                                                 cGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCg                                                                                     <  2:198969/136‑1 (MQ=255)
                                                  ggaaaCATCCACGCCGGCTACACGCGCGAGGGCAAGGCCGTGCGGGGCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAATGACGCGCTGCAGCCGAACATGGTGCAGAAGCTGGAGAATAATCCg                                                                                     <  5:334404/132‑1 (MQ=255)
                                                  ggCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCgg                                                                                    <  7:279450/136‑1 (MQ=255)
                                                       cATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTc                                                                               <  4:262979/136‑1 (MQ=255)
                                                               ccGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATgg                                                                       <  8:176743/136‑1 (MQ=255)
                                                                 ggCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATggcg                                                                     <  3:395743/136‑1 (MQ=255)
                                                                 ggCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATggcg                                                                     <  4:327690/136‑1 (MQ=255)
                                                                     acacGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGccc                                                                 >  1:207934/1‑136 (MQ=255)
                                                                      cacGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCg                                                                >  1:188458/1‑136 (MQ=255)
                                                                        cgcgcgAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCGGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGtt                                                              <  6:82709/136‑1 (MQ=255)
                                                                         gcgcgAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCGTCGGGCGCCATGACCGTGCTGCTCAAGGACGCTCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCTTGGCGGCCCGTTc                                                             <  1:96041/136‑1 (MQ=255)
                                                                           gcgAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGCAGAATAATCCGGTGCTCATCCATGGCGGACCGTTcgc                                                           >  5:52161/1‑136 (MQ=255)
                                                                                   aaGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATcg                                                   <  1:39058/136‑1 (MQ=255)
                                                                                     ggCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATcgcg                                                 <  7:128528/136‑1 (MQ=255)
                                                                                             tGGCCAGCGAGGTGAATGCCTCGGGCGCCATGACCGTGCTGCTAAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGAGATCATCCATGGCGGCACCATCGCGAACATCGCGCATGGc                                           >  7:317486/1‑134 (MQ=255)
                                                                                             tGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTg                                         >  8:96084/1‑136 (MQ=255)
                                                                                             tGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTg                                         >  2:89001/1‑136 (MQ=255)
                                                                                             tGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTg                                         >  3:189948/1‑136 (MQ=255)
                                                                                             tGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCGGCTCAAGGACGCGCTGc                                                                                                                        <  8:118589/57‑1 (MQ=255)
                                                                                             tGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCGGCTCAAGGACGCGCTGc                                                                                                                        >  7:118589/1‑57 (MQ=255)
                                                                                             tGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCGGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTg                                         >  4:277010/1‑136 (MQ=255)
                                                                                                  aGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGTGAACATCGCGCATGGCTGCAACt                                    >  1:39071/1‑136 (MQ=255)
                                                                                                   gCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGACTGCAACTc                                   <  5:106638/136‑1 (MQ=255)
                                                                                                    cGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGTGCATGGCTGCAACTCg                                  >  7:3426/1‑136 (MQ=255)
                                                                                                       gCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTg                               >  7:118954/1‑136 (MQ=255)
                                                                                                       gCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTg                               <  1:243135/136‑1 (MQ=255)
                                                                                                       gCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTg                               >  2:243135/1‑136 (MQ=255)
                                                                                                       gCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTg                               >  5:201918/1‑136 (MQ=255)
                                                                                                       gCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTg                               <  5:6676/136‑1 (MQ=255)
                                                                                                       gCTGAATGCCTCGGGCGCCATGACCGTGCGGCTCAAGGACGCGCTGCAGCCTAACATGGGGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTg                               <  6:388834/136‑1 (MQ=255)
                                                                                                                             aCCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCCCCAAGCCCGCGCTc          >  6:292283/1‑135 (MQ=255)
                                                                                                                                 tgctgcTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGAt     <  7:18852/136‑1 (MQ=255)
                                                                                                                                 tgctgcTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGAt     <  5:163888/136‑1 (MQ=255)
                                                                                                                                  gctgctCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATTGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGAt     <  3:353945/135‑1 (MQ=255)
                                                                                                                                    tgctCAAGTACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCCCCCAGACCGAGCTCAAGATCGc  >  2:97072/1‑136 (MQ=255)
                                                                                                                                    tgctCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGc  >  3:187263/1‑136 (MQ=255)
                                                                                                                                    tgctCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGc  >  6:218553/1‑136 (MQ=255)
                                                                                                                                    tgctCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGc  >  4:50035/1‑136 (MQ=255)
                                                                                                                                    tgctCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGc  >  2:229551/1‑136 (MQ=255)
                                                                                                                                    tgctCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGc  >  5:55640/1‑136 (MQ=255)
                                                                                                                                    |                                                                                                                                       
TGGCGATCCTGTGCCTTTCCTCGGACCTCAAGGACCTCGAGCGGCGCCTCGGCAACATCCACGCCGGCTACACGCGCGAGCGCAAGGCCGTGCTGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGC  >  ACB143/5390520‑5390787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑