Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,391,513 G→T 12.8% G536V (GGC→GTC)  ligH → 10‑formyltetrahydrofolate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,391,5130GT12.8% 212.1 / 16.0 75G536V (GGC→GTC) ligH10‑formyltetrahydrofolate synthetase
Reads supporting (aligned to +/- strand):  ref base G (30/35);  new base T (5/5);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

CGCAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATAAGGCCTCCCTAACGGGGGGCCTTTTTTATTGAT  >  ACB143/5391378‑5391640
                                                                                                                                       |                                                                                                                               
cGCAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCgg                                                                                                                                 >  1:82780/1‑136 (MQ=255)
  cAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGcc                                                                                                                               >  5:225353/1‑136 (MQ=255)
      aCAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGCCGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGcc                                                                                                                           >  1:395862/1‑136 (MQ=255)
      aCAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGCCGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGcc                                                                                                                           >  2:204040/1‑136 (MQ=255)
      aCAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGcc                                                                                                                           >  3:314938/1‑136 (MQ=255)
       cAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGccc                                                                                                                          >  1:152801/1‑136 (MQ=255)
         gCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCaa                                                                                                                        <  1:180573/136‑1 (MQ=255)
             ctcGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCaa                                                                                                                        <  5:132372/132‑1 (MQ=255)
             ctcGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCaa                                                                                                                        >  6:132372/1‑132 (MQ=255)
             ctcGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTc                                                                                                                    >  3:79980/1‑136 (MQ=255)
                gaccgaccCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTcccc                                                                                                                 >  4:398536/1‑136 (MQ=255)
                gaccgaccCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTcccc                                                                                                                 >  3:150751/1‑136 (MQ=255)
                 accgaccCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCt                                                                                                                <  6:261816/136‑1 (MQ=255)
                  ccgaccCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTc                                                                                                               >  7:208005/1‑136 (MQ=255)
                        cGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGa                                                                                                         >  5:284389/1‑136 (MQ=255)
                        cGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGa                                                                                                         >  3:19182/1‑136 (MQ=255)
                        cGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGATCCCCTCGGCAGa                                                                                                         >  7:377556/1‑136 (MQ=255)
                           cgcTGCGCGGCGGCCCGACCGACCATGTCGTGCCCGTGCGCGTCGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAATGTCCCCTCGGCAGACtt                                                                                                      <  5:256751/136‑1 (MQ=255)
                                    gcgcCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCt                                                                                             <  4:61523/136‑1 (MQ=255)
                                        cccGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTcgac                                                                                         >  2:201020/1‑136 (MQ=255)
                                        cccGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTcgac                                                                                         <  1:195382/136‑1 (MQ=255)
                                         ccgaccgaCCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTcgacg                                                                                        <  6:44782/136‑1 (MQ=255)
                                         ccgaccgaCCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTcgacg                                                                                        <  1:64366/136‑1 (MQ=255)
                                         ccgaccgaCCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTcgacg                                                                                        <  6:40225/136‑1 (MQ=255)
                                         ccgaccgaCCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTcgacg                                                                                        <  8:272297/136‑1 (MQ=255)
                                          cgaccgacCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTcgacga                                                                                       <  7:50140/136‑1 (MQ=255)
                                             ccgaccATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCGCGGCAGACTTCATCAAGCTCGACGAg                                                                                      >  2:308710/1‑134 (MQ=255)
                                              cgaccATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGTCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAg                                                                                   <  3:114683/136‑1 (MQ=255)
                                              cgaccATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGTCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAg                                                                                   <  6:384662/136‑1 (MQ=255)
                                              cgaccATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAg                                                                                   <  8:257700/136‑1 (MQ=255)
                                              cgaccATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAg                                                                                   <  5:287387/136‑1 (MQ=255)
                                              cgaccATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAg                                                                                   <  1:9117/136‑1 (MQ=255)
                                                accCTGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAggg                                                                                 <  8:97781/136‑1 (MQ=255)
                                                  cATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGGTCATCGTGGCGGTCTGCGGCGCCATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGc                                                                                >  2:187228/1‑135 (MQ=255)
                                                         tGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATc                                                                          >  1:24690/1‑134 (MQ=255)
                                                          gCCCGTGCTCGACGTGATGCTCTCGGGCGGGGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGACCAAGGTCCCCTCGGCAGACTTCATCCAGCTCGACGAGCAGGGCCAGATCCAg                                                                       <  3:270609/136‑1 (MQ=255)
                                                           cccGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAg                                                                       <  5:275686/135‑1 (MQ=255)
                                                           cccGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAg                                                                       <  7:161456/135‑1 (MQ=255)
                                                           cccGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAg                                                                       <  2:94385/135‑1 (MQ=255)
                                                           cccGGGCGCGACGTGATCCTCTCGGACGGCTCGGAGTTGATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCATCGGCAGACTTCATCCAGCTCGACGAGCAGGGCCAGATCCAg                                                                       <  8:102881/135‑1 (MQ=255)
                                                              gTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGCCATCATGCGCATGCCCGTCCTGCCCAACGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGc                                                                    >  8:118068/1‑135 (MQ=255)
                                                              gTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGTCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGcc                                                                   >  2:57031/1‑136 (MQ=255)
                                                              gTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGTCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGcc                                                                   >  3:214385/1‑136 (MQ=255)
                                                              gTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGcc                                                                   >  4:280286/1‑136 (MQ=255)
                                                              gTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGcc                                                                   >  2:53713/1‑136 (MQ=255)
                                                              gTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGcc                                                                   >  2:373447/1‑136 (MQ=255)
                                                              gTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGcc                                                                   >  5:389134/1‑136 (MQ=255)
                                                                  gcgACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGtt                                                               >  1:95068/1‑136 (MQ=255)
                                                                  gcgACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGtt                                                               >  3:299353/1‑136 (MQ=255)
                                                                  gcgACGTGATCCTATCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGtt                                                               >  5:391420/1‑136 (MQ=255)
                                                                       gTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAg                                                          <  6:369529/136‑1 (MQ=255)
                                                                       gTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAg                                                          <  4:201970/136‑1 (MQ=255)
                                                                       gTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAg                                                          <  2:152801/136‑1 (MQ=255)
                                                                       gTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAg                                                          <  5:344059/136‑1 (MQ=255)
                                                                             ctctCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGTCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGACCAGATCCAGGGCCTGTTCTGAGCCCCg                                                     <  3:19705/135‑1 (MQ=255)
                                                                                    cctgcgcGGAGTTCATCCTGGCGGTCTGCGGCGACATCATGAGCATGAGAGGCCTTACCAAGGTCACCTCGGAAGACTTCATCAAGCTCGACGAGCAGGGGAAGATCGAGGGCATGTTCTGATCCCCGGCCATggg                                             <  2:225193/133‑1 (MQ=255)
                                                                                    ccggcGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGTCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATggg                                             <  7:37363/136‑1 (MQ=255)
                                                                                    ccggcGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATggg                                             <  6:225353/136‑1 (MQ=255)
                                                                                    ccggcGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATggg                                             <  7:16746/136‑1 (MQ=255)
                                                                                    ccggcGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATggg                                             <  2:62054/136‑1 (MQ=255)
                                                                                    ccggcGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATgg                                              >  5:268225/1‑135 (MQ=255)
                                                                                           ggAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCt                                                                                                                              >  8:298134/1‑48 (MQ=255)
                                                                                           ggAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCt                                                                                                                              <  7:298134/48‑1 (MQ=255)
                                                                                           ggAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAAc                                       >  5:88505/1‑135 (MQ=255)
                                                                                               ttCATCGTGGCGGTCTGCGGCGACATGATGGGCATGCCCGGCGTGCACAAGGTACCCTCGGAAGTCTTCATCAAGATCGACGAGCAGGGCCATATCGAGGGGCTGTTCTGAGCGCCGGCTATGGGACGAAGAATaa                                  <  5:311459/136‑1 (MQ=255)
                                                                                               ttCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGTCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATaa                                  <  8:207293/136‑1 (MQ=255)
                                                                                               ttCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATaa                                  <  8:244790/136‑1 (MQ=255)
                                                                                               ttCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATaa                                  <  3:398536/136‑1 (MQ=255)
                                                                                               ttCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATaa                                  <  6:284389/136‑1 (MQ=255)
                                                                                                  aTCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATAAgg                                <  1:201020/135‑1 (MQ=255)
                                                                                                    cGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCAATGGGACGAACAATAAGGCCt                             >  7:23883/1‑136 (MQ=255)
                                                                                                          ggTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAg                                                                       >  3:190002/1‑88 (MQ=255)
                                                                                                          ggTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAg                                                                       <  4:190002/88‑1 (MQ=255)
                                                                                                          ggTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATAAGGCCTCCCTaa                       >  3:369154/1‑136 (MQ=255)
                                                                                                                              gcATGCCCGTCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATAAGGCCTCCCTAACGGGGGGCCTTTTTTATTGa   >  7:326016/1‑136 (MQ=255)
                                                                                                                                aTGCCCGTCCTGCCCAAGATCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGATCTGAGCCCCGGCCATGGGACGAACAATAAGGCATCCCTAACGGGCGGCCTTTTTTATTGAt  >  8:249226/1‑135 (MQ=255)
                                                                                                                                       |                                                                                                                               
CGCAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATAAGGCCTCCCTAACGGGGGGCCTTTTTTATTGAT  >  ACB143/5391378‑5391640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑